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Bovine feces from animals with gastrointestinal infections are a source of serologically diverse atypical enteropathogenic Escherichia coli and Shiga toxin-producing E. coli strains that commonly possess intimin.
Appl Environ Microbiol. 2005 Jul; 71(7):3405-12.AE

Abstract

Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) cells were isolated from 191 fecal samples from cattle with gastrointestinal infections (diagnostic samples) collected in New South Wales, Australia. By using a multiplex PCR, E. coli cells possessing combinations of stx1, stx2, eae, and ehxA were detected by a combination of direct culture and enrichment in E. coli (EC) (modified) broth followed by plating on vancomycin-cefixime-cefsulodin blood (BVCC) agar for the presence of enterohemolytic colonies and on sorbitol MacConkey agar for the presence of non-sorbitol-fermenting colonies. The high prevalence of the intimin gene eae was a feature of the STEC (35 [29.2%] of 120 isolates) and contrasted with the low prevalence (9 [0.5%] of 1,692 fecal samples possessed STEC with eae) of this gene among STEC recovered during extensive sampling of feces from healthy slaughter-age cattle in Australia (M. Hornitzky, B. A. Vanselow, K. Walker, K. A. Bettelheim, B. Corney, P. Gill, G. Bailey, and S. P. Djordjevic, Appl. Environ. Microbiol. 68:6439-6445, 2002). Forty-seven STEC serotypes were identified, including O5:H-, O8:H19, O26:H-, O26:H11, O113:H21, O157:H7, O157:H- and Ont:H- which are known to cause severe disease in humans and 23 previously unreported STEC serotypes. Serotypes Ont:H- and O113:H21 represented the two most frequently isolated STEC isolates and were cultured from nine (4.7%) and seven (3.7%) animals, respectively. Fifteen eae-positive E. coli serotypes, considered to represent atypical EPEC, were identified, with O111:H- representing the most prevalent. Using both techniques, STEC cells were cultured from 69 (36.1%) samples and EPEC cells were cultured from 30 (15.7%) samples, including 9 (4.7%) samples which yielded both STEC and EPEC. Culture on BVCC agar following enrichment in EC (modified) broth was the most successful method for the isolation of STEC (24.1% of samples), and direct culture on BVCC agar was the most successful method for the isolation of EPEC (14.1% samples). These studies show that diarrheagenic calves and cattle represent important reservoirs of eae-positive E. coli.

Authors+Show Affiliations

Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Private Mail Bag 8, Camden, New South Wales 2570, Australia. michael.hornitzky@agric.nsw.gov.auNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16000742

Citation

Hornitzky, Michael A., et al. "Bovine Feces From Animals With Gastrointestinal Infections Are a Source of Serologically Diverse Atypical Enteropathogenic Escherichia Coli and Shiga Toxin-producing E. Coli Strains That Commonly Possess Intimin." Applied and Environmental Microbiology, vol. 71, no. 7, 2005, pp. 3405-12.
Hornitzky MA, Mercieca K, Bettelheim KA, et al. Bovine feces from animals with gastrointestinal infections are a source of serologically diverse atypical enteropathogenic Escherichia coli and Shiga toxin-producing E. coli strains that commonly possess intimin. Appl Environ Microbiol. 2005;71(7):3405-12.
Hornitzky, M. A., Mercieca, K., Bettelheim, K. A., & Djordjevic, S. P. (2005). Bovine feces from animals with gastrointestinal infections are a source of serologically diverse atypical enteropathogenic Escherichia coli and Shiga toxin-producing E. coli strains that commonly possess intimin. Applied and Environmental Microbiology, 71(7), 3405-12.
Hornitzky MA, et al. Bovine Feces From Animals With Gastrointestinal Infections Are a Source of Serologically Diverse Atypical Enteropathogenic Escherichia Coli and Shiga Toxin-producing E. Coli Strains That Commonly Possess Intimin. Appl Environ Microbiol. 2005;71(7):3405-12. PubMed PMID: 16000742.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bovine feces from animals with gastrointestinal infections are a source of serologically diverse atypical enteropathogenic Escherichia coli and Shiga toxin-producing E. coli strains that commonly possess intimin. AU - Hornitzky,Michael A, AU - Mercieca,Kim, AU - Bettelheim,Karl A, AU - Djordjevic,Steven P, PY - 2005/7/8/pubmed PY - 2005/9/15/medline PY - 2005/7/8/entrez SP - 3405 EP - 12 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 71 IS - 7 N2 - Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) cells were isolated from 191 fecal samples from cattle with gastrointestinal infections (diagnostic samples) collected in New South Wales, Australia. By using a multiplex PCR, E. coli cells possessing combinations of stx1, stx2, eae, and ehxA were detected by a combination of direct culture and enrichment in E. coli (EC) (modified) broth followed by plating on vancomycin-cefixime-cefsulodin blood (BVCC) agar for the presence of enterohemolytic colonies and on sorbitol MacConkey agar for the presence of non-sorbitol-fermenting colonies. The high prevalence of the intimin gene eae was a feature of the STEC (35 [29.2%] of 120 isolates) and contrasted with the low prevalence (9 [0.5%] of 1,692 fecal samples possessed STEC with eae) of this gene among STEC recovered during extensive sampling of feces from healthy slaughter-age cattle in Australia (M. Hornitzky, B. A. Vanselow, K. Walker, K. A. Bettelheim, B. Corney, P. Gill, G. Bailey, and S. P. Djordjevic, Appl. Environ. Microbiol. 68:6439-6445, 2002). Forty-seven STEC serotypes were identified, including O5:H-, O8:H19, O26:H-, O26:H11, O113:H21, O157:H7, O157:H- and Ont:H- which are known to cause severe disease in humans and 23 previously unreported STEC serotypes. Serotypes Ont:H- and O113:H21 represented the two most frequently isolated STEC isolates and were cultured from nine (4.7%) and seven (3.7%) animals, respectively. Fifteen eae-positive E. coli serotypes, considered to represent atypical EPEC, were identified, with O111:H- representing the most prevalent. Using both techniques, STEC cells were cultured from 69 (36.1%) samples and EPEC cells were cultured from 30 (15.7%) samples, including 9 (4.7%) samples which yielded both STEC and EPEC. Culture on BVCC agar following enrichment in EC (modified) broth was the most successful method for the isolation of STEC (24.1% of samples), and direct culture on BVCC agar was the most successful method for the isolation of EPEC (14.1% samples). These studies show that diarrheagenic calves and cattle represent important reservoirs of eae-positive E. coli. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/16000742/Bovine_feces_from_animals_with_gastrointestinal_infections_are_a_source_of_serologically_diverse_atypical_enteropathogenic_Escherichia_coli_and_Shiga_toxin_producing_E__coli_strains_that_commonly_possess_intimin_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=16000742 DB - PRIME DP - Unbound Medicine ER -