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Role of RNA structures present at the 3'UTR of dengue virus on translation, RNA synthesis, and viral replication.
Virology. 2005 Sep 01; 339(2):200-12.V

Abstract

We have developed a dengue virus replicon system that can be used to discriminate between translation and RNA replication. Using this system, we analyzed the functional role of well-defined RNA elements present at the 3'UTR of dengue virus in mammalian and mosquito cells. Our results show that deletion of individual domains of the 3'UTR did not significantly affect translation of the input RNA but seriously compromised or abolished RNA synthesis. We demonstrated that complementarity between sequences present at the 5' and 3' ends of the genome is essential for dengue virus RNA synthesis, while deletion of domains A2 or A3 within the 3'UTR resulted in replicons with decreased RNA amplification. We also characterized the vaccine candidate rDEN2Delta30 in the replicon system and found that viral attenuation is caused by inefficient RNA synthesis. Furthermore, using both the replicon system and recombinant viruses, we identified an RNA region of the 3'UTR that enhances dengue virus replication in BHK cells while is dispensable in mosquito cells.

Authors+Show Affiliations

Fundación Instituto Leloir, Avenida Patricias Argentinas 435, Buenos Aires 1405, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16002117

Citation

Alvarez, Diego E., et al. "Role of RNA Structures Present at the 3'UTR of Dengue Virus On Translation, RNA Synthesis, and Viral Replication." Virology, vol. 339, no. 2, 2005, pp. 200-12.
Alvarez DE, De Lella Ezcurra AL, Fucito S, et al. Role of RNA structures present at the 3'UTR of dengue virus on translation, RNA synthesis, and viral replication. Virology. 2005;339(2):200-12.
Alvarez, D. E., De Lella Ezcurra, A. L., Fucito, S., & Gamarnik, A. V. (2005). Role of RNA structures present at the 3'UTR of dengue virus on translation, RNA synthesis, and viral replication. Virology, 339(2), 200-12.
Alvarez DE, et al. Role of RNA Structures Present at the 3'UTR of Dengue Virus On Translation, RNA Synthesis, and Viral Replication. Virology. 2005 Sep 1;339(2):200-12. PubMed PMID: 16002117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of RNA structures present at the 3'UTR of dengue virus on translation, RNA synthesis, and viral replication. AU - Alvarez,Diego E, AU - De Lella Ezcurra,Ana Laura, AU - Fucito,Silvana, AU - Gamarnik,Andrea V, PY - 2005/03/31/received PY - 2005/04/19/revised PY - 2005/06/02/accepted PY - 2005/7/9/pubmed PY - 2005/11/15/medline PY - 2005/7/9/entrez SP - 200 EP - 12 JF - Virology JO - Virology VL - 339 IS - 2 N2 - We have developed a dengue virus replicon system that can be used to discriminate between translation and RNA replication. Using this system, we analyzed the functional role of well-defined RNA elements present at the 3'UTR of dengue virus in mammalian and mosquito cells. Our results show that deletion of individual domains of the 3'UTR did not significantly affect translation of the input RNA but seriously compromised or abolished RNA synthesis. We demonstrated that complementarity between sequences present at the 5' and 3' ends of the genome is essential for dengue virus RNA synthesis, while deletion of domains A2 or A3 within the 3'UTR resulted in replicons with decreased RNA amplification. We also characterized the vaccine candidate rDEN2Delta30 in the replicon system and found that viral attenuation is caused by inefficient RNA synthesis. Furthermore, using both the replicon system and recombinant viruses, we identified an RNA region of the 3'UTR that enhances dengue virus replication in BHK cells while is dispensable in mosquito cells. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/16002117/Role_of_RNA_structures_present_at_the_3'UTR_of_dengue_virus_on_translation_RNA_synthesis_and_viral_replication_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(05)00337-5 DB - PRIME DP - Unbound Medicine ER -