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Biological and immunological properties of the venom of Bothrops alcatraz, an endemic species of pitviper from Brazil.
Comp Biochem Physiol C Toxicol Pharmacol. 2005 Jun; 141(2):117-23.CB

Abstract

Bothrops alcatraz is a new pitviper species derived from the Bothrops jararaca group, whose natural habitat is situated in Alcatrazes Archipelago, a group of marine islands near São Paulo State coast in Brazil. Herein, the biological and biochemical properties of venoms of four adult specimens of B. alcatraz were examined comparatively to a reference pool of Bothrops jararaca venom. Both venoms showed similar activities and electrophoretic patterns, but B. alcatraz venom showed three protein bands of molecular masses of 97, 80 and 38 kDa that were not present in B. jararaca reference venom. The i.p. median lethal dose of B. alcatraz venom ranged from 5.1 to 6.6 mg/kg, while it was 1.5 mg/kg for B. jararaca venom. The minimum hemorrhagic dose of B. jararaca venom was 0.63, whereas 2.28 mug/mouse for B. alcatraz venom. In contrast, B. alcatraz venom was more potent in regard to procoagulant and proteolytic activities. These differences were supported by western blotting and neutralization tests, employing commercial bothropic antivenom, which showed that hemorrhagic and lethal activities of B. alcatraz venom were less effectively inhibited than B. jararaca venom. Such results evidence that B. alcatraz shows quantitative and qualitative differences in venom composition in comparison with its B. jararaca relatives, which might represent an optimization of venom towards a specialized diet.

Authors+Show Affiliations

Laboratório de Herpetologia, Instituto Butantan, Av. Vital Brasil, 1500, 05503-900, São Paulo, SP, Brazil. fatifurtado@butantan.gov.br

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16002343

Citation

Furtado, M F D.. "Biological and Immunological Properties of the Venom of Bothrops Alcatraz, an Endemic Species of Pitviper From Brazil." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 141, no. 2, 2005, pp. 117-23.
Furtado MF. Biological and immunological properties of the venom of Bothrops alcatraz, an endemic species of pitviper from Brazil. Comp Biochem Physiol C Toxicol Pharmacol. 2005;141(2):117-23.
Furtado, M. F. (2005). Biological and immunological properties of the venom of Bothrops alcatraz, an endemic species of pitviper from Brazil. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 141(2), 117-23.
Furtado MF. Biological and Immunological Properties of the Venom of Bothrops Alcatraz, an Endemic Species of Pitviper From Brazil. Comp Biochem Physiol C Toxicol Pharmacol. 2005;141(2):117-23. PubMed PMID: 16002343.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biological and immunological properties of the venom of Bothrops alcatraz, an endemic species of pitviper from Brazil. A1 - Furtado,M F D, PY - 2003/12/12/received PY - 2004/09/19/revised PY - 2004/09/20/accepted PY - 2005/7/9/pubmed PY - 2005/10/12/medline PY - 2005/7/9/entrez SP - 117 EP - 23 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 141 IS - 2 N2 - Bothrops alcatraz is a new pitviper species derived from the Bothrops jararaca group, whose natural habitat is situated in Alcatrazes Archipelago, a group of marine islands near São Paulo State coast in Brazil. Herein, the biological and biochemical properties of venoms of four adult specimens of B. alcatraz were examined comparatively to a reference pool of Bothrops jararaca venom. Both venoms showed similar activities and electrophoretic patterns, but B. alcatraz venom showed three protein bands of molecular masses of 97, 80 and 38 kDa that were not present in B. jararaca reference venom. The i.p. median lethal dose of B. alcatraz venom ranged from 5.1 to 6.6 mg/kg, while it was 1.5 mg/kg for B. jararaca venom. The minimum hemorrhagic dose of B. jararaca venom was 0.63, whereas 2.28 mug/mouse for B. alcatraz venom. In contrast, B. alcatraz venom was more potent in regard to procoagulant and proteolytic activities. These differences were supported by western blotting and neutralization tests, employing commercial bothropic antivenom, which showed that hemorrhagic and lethal activities of B. alcatraz venom were less effectively inhibited than B. jararaca venom. Such results evidence that B. alcatraz shows quantitative and qualitative differences in venom composition in comparison with its B. jararaca relatives, which might represent an optimization of venom towards a specialized diet. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/16002343/Biological_and_immunological_properties_of_the_venom_of_Bothrops_alcatraz_an_endemic_species_of_pitviper_from_Brazil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(05)00123-7 DB - PRIME DP - Unbound Medicine ER -