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The solution structure of a transient photoreceptor intermediate: Delta25 photoactive yellow protein.
Structure. 2005 Jul; 13(7):953-62.S

Abstract

The N-terminally truncated variant of photoactive yellow protein (Delta25-PYP) undergoes a very similar photocycle as the corresponding wild-type protein (WT-PYP), although the lifetime of its light-illuminated (pB) state is much longer. This has allowed determination of the structure of both its dark- (pG) as well as its pB-state in solution by nuclear magnetic resonance (NMR) spectroscopy. The pG structure shows a well-defined fold, similar to WT-PYP and the X-ray structure of the pG state of Delta25-PYP. In the long-lived photocycle intermediate pB, the central beta sheet is still intact, as well as a small part of one alpha helix. The remainder of pB is unfolded and highly flexible, as evidenced by results from proton-deuterium exchange and NMR relaxation studies. Thus, the partially unfolded nature of the presumed signaling state of PYP in solution, as suggested previously, has now been structurally demonstrated.

Authors+Show Affiliations

Department of NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16004868

Citation

Bernard, Cédric, et al. "The Solution Structure of a Transient Photoreceptor Intermediate: Delta25 Photoactive Yellow Protein." Structure (London, England : 1993), vol. 13, no. 7, 2005, pp. 953-62.
Bernard C, Houben K, Derix NM, et al. The solution structure of a transient photoreceptor intermediate: Delta25 photoactive yellow protein. Structure. 2005;13(7):953-62.
Bernard, C., Houben, K., Derix, N. M., Marks, D., van der Horst, M. A., Hellingwerf, K. J., Boelens, R., Kaptein, R., & van Nuland, N. A. (2005). The solution structure of a transient photoreceptor intermediate: Delta25 photoactive yellow protein. Structure (London, England : 1993), 13(7), 953-62.
Bernard C, et al. The Solution Structure of a Transient Photoreceptor Intermediate: Delta25 Photoactive Yellow Protein. Structure. 2005;13(7):953-62. PubMed PMID: 16004868.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The solution structure of a transient photoreceptor intermediate: Delta25 photoactive yellow protein. AU - Bernard,Cédric, AU - Houben,Klaartje, AU - Derix,Nocky M, AU - Marks,David, AU - van der Horst,Michael A, AU - Hellingwerf,Klaas J, AU - Boelens,Rolf, AU - Kaptein,Robert, AU - van Nuland,Nico A J, PY - 2004/12/24/received PY - 2005/04/23/revised PY - 2005/04/23/accepted PY - 2005/7/12/pubmed PY - 2005/12/13/medline PY - 2005/7/12/entrez SP - 953 EP - 62 JF - Structure (London, England : 1993) JO - Structure VL - 13 IS - 7 N2 - The N-terminally truncated variant of photoactive yellow protein (Delta25-PYP) undergoes a very similar photocycle as the corresponding wild-type protein (WT-PYP), although the lifetime of its light-illuminated (pB) state is much longer. This has allowed determination of the structure of both its dark- (pG) as well as its pB-state in solution by nuclear magnetic resonance (NMR) spectroscopy. The pG structure shows a well-defined fold, similar to WT-PYP and the X-ray structure of the pG state of Delta25-PYP. In the long-lived photocycle intermediate pB, the central beta sheet is still intact, as well as a small part of one alpha helix. The remainder of pB is unfolded and highly flexible, as evidenced by results from proton-deuterium exchange and NMR relaxation studies. Thus, the partially unfolded nature of the presumed signaling state of PYP in solution, as suggested previously, has now been structurally demonstrated. SN - 0969-2126 UR - https://www.unboundmedicine.com/medline/citation/16004868/The_solution_structure_of_a_transient_photoreceptor_intermediate:_Delta25_photoactive_yellow_protein_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969-2126(05)00182-6 DB - PRIME DP - Unbound Medicine ER -