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Detection of d-Serine in rat brain by capillary electrophoresis with laser induced fluorescence detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Aug 05; 822(1-2):334-8.JC

Abstract

A capillary zone electrophoresis method with laser induced fluorescence detection for the chiral separation of highly fluorescent enantiomeric derivatives of d/l-Serine from 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-d/l-Serine) was developed and optimized. Enantiomeric separation of NBD-d/l-Serine was accomplished by using 40 mM hydroxypropyl-beta-cyclodextrin (HP-beta-CD) contained in 100 mM borate buffer, pH 10.0. A 70 cm (effective length of 50 cm) uncoated fused-silica capillary at a voltage of 15 kV was used for the separation. The optimized electrophoretic conditions were subsequently applied to the analysis of d-Serine in rat brain, and satisfactory analytical results with respect to accuracy were obtained. This assay showed acceptable precision, with linearity in the d-Serine concentration range of 0.2-20.0 microM. The limit of detection for d-Serine was 3.0 x 10(-7)M.

Authors+Show Affiliations

College of Chemical Engineering, Sichuan University, Chengdu 610065, China. zhaoshulin001@163.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16005268

Citation

Zhao, Shulin, et al. "Detection of d-Serine in Rat Brain By Capillary Electrophoresis With Laser Induced Fluorescence Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 822, no. 1-2, 2005, pp. 334-8.
Zhao S, Yuan H, Xiao D. Detection of d-Serine in rat brain by capillary electrophoresis with laser induced fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2005;822(1-2):334-8.
Zhao, S., Yuan, H., & Xiao, D. (2005). Detection of d-Serine in rat brain by capillary electrophoresis with laser induced fluorescence detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 822(1-2), 334-8.
Zhao S, Yuan H, Xiao D. Detection of d-Serine in Rat Brain By Capillary Electrophoresis With Laser Induced Fluorescence Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Aug 5;822(1-2):334-8. PubMed PMID: 16005268.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of d-Serine in rat brain by capillary electrophoresis with laser induced fluorescence detection. AU - Zhao,Shulin, AU - Yuan,Hongyan, AU - Xiao,Dan, PY - 2004/12/16/received PY - 2005/05/30/revised PY - 2005/06/09/accepted PY - 2005/7/12/pubmed PY - 2005/10/1/medline PY - 2005/7/12/entrez SP - 334 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 822 IS - 1-2 N2 - A capillary zone electrophoresis method with laser induced fluorescence detection for the chiral separation of highly fluorescent enantiomeric derivatives of d/l-Serine from 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-d/l-Serine) was developed and optimized. Enantiomeric separation of NBD-d/l-Serine was accomplished by using 40 mM hydroxypropyl-beta-cyclodextrin (HP-beta-CD) contained in 100 mM borate buffer, pH 10.0. A 70 cm (effective length of 50 cm) uncoated fused-silica capillary at a voltage of 15 kV was used for the separation. The optimized electrophoretic conditions were subsequently applied to the analysis of d-Serine in rat brain, and satisfactory analytical results with respect to accuracy were obtained. This assay showed acceptable precision, with linearity in the d-Serine concentration range of 0.2-20.0 microM. The limit of detection for d-Serine was 3.0 x 10(-7)M. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16005268/Detection_of_d_Serine_in_rat_brain_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(05)00436-8 DB - PRIME DP - Unbound Medicine ER -