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1alpha,25-Dihydroxyvitamin D3 regulates the expression of Id1 and Id2 genes and the angiogenic phenotype of human colon carcinoma cells.
Oncogene. 2005 Sep 29; 24(43):6533-44.O

Abstract

1alpha,25-Dihydroxyvitamin D3 (1alpha,25(OH)2D3) has antitumor activity in addition to its classical action on calcium metabolism and bone tissue biology. It is thought to regulate the expression of multiple target genes and thus modulate processes critical for tumor growth and metastases. Here we show that 1alpha,25(OH)2D3 differentially regulates the expression of Id1 and Id2 genes, members of a family of transcriptional regulators of cell proliferation and differentiation. 1alpha,25(OH)2D3 induced epithelial differentiation in SW480-ADH human colon carcinoma cell line by promoting expression of the proteins implicated in adherent junction formation, including E-cadherin, and by inhibiting beta-catenin transcriptional activity. 1alpha,25(OH)2D3 activated the human Id1 gene promoter and rapidly induced Id1 RNA and protein. Ectopic overexpression of Id1 was not sufficient to induce E-cadherin, which was critical for the morphological changes induced by 1alpha,25(OH)2D3 in SW480-ADH cells. Conversely, Id2 transcription rate, RNA and protein levels were decreased by 1alpha,25(OH)2D3. Id2 downregulation by 1alpha,25(OH)2D3 mediated the antiproliferative effect of 1alpha,25(OH)2D3 on SW480-ADH cells. In addition, we showed that 1alpha,25(OH)2D3 changed the levels of the inducer of angiogenesis, vascular endothelial growth factor and the potent antiangiogenic factor thrombospondin-1, leading to a balanced change in the angiogenic potential of SW480-ADH human colon carcinoma cells.

Authors+Show Affiliations

Instituto de Investigaciones Biomedicas CSIC-UAM, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16007183

Citation

Fernandez-Garcia, Nuria Isabel, et al. "1alpha,25-Dihydroxyvitamin D3 Regulates the Expression of Id1 and Id2 Genes and the Angiogenic Phenotype of Human Colon Carcinoma Cells." Oncogene, vol. 24, no. 43, 2005, pp. 6533-44.
Fernandez-Garcia NI, Palmer HG, Garcia M, et al. 1alpha,25-Dihydroxyvitamin D3 regulates the expression of Id1 and Id2 genes and the angiogenic phenotype of human colon carcinoma cells. Oncogene. 2005;24(43):6533-44.
Fernandez-Garcia, N. I., Palmer, H. G., Garcia, M., Gonzalez-Martin, A., del Rio, M., Barettino, D., Volpert, O., Muñoz, A., & Jimenez, B. (2005). 1alpha,25-Dihydroxyvitamin D3 regulates the expression of Id1 and Id2 genes and the angiogenic phenotype of human colon carcinoma cells. Oncogene, 24(43), 6533-44.
Fernandez-Garcia NI, et al. 1alpha,25-Dihydroxyvitamin D3 Regulates the Expression of Id1 and Id2 Genes and the Angiogenic Phenotype of Human Colon Carcinoma Cells. Oncogene. 2005 Sep 29;24(43):6533-44. PubMed PMID: 16007183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 1alpha,25-Dihydroxyvitamin D3 regulates the expression of Id1 and Id2 genes and the angiogenic phenotype of human colon carcinoma cells. AU - Fernandez-Garcia,Nuria Isabel, AU - Palmer,Hector G, AU - Garcia,Marta, AU - Gonzalez-Martin,Alicia, AU - del Rio,Marcela, AU - Barettino,Domingo, AU - Volpert,Olga, AU - Muñoz,Alberto, AU - Jimenez,Benilde, PY - 2005/7/12/pubmed PY - 2005/10/21/medline PY - 2005/7/12/entrez SP - 6533 EP - 44 JF - Oncogene JO - Oncogene VL - 24 IS - 43 N2 - 1alpha,25-Dihydroxyvitamin D3 (1alpha,25(OH)2D3) has antitumor activity in addition to its classical action on calcium metabolism and bone tissue biology. It is thought to regulate the expression of multiple target genes and thus modulate processes critical for tumor growth and metastases. Here we show that 1alpha,25(OH)2D3 differentially regulates the expression of Id1 and Id2 genes, members of a family of transcriptional regulators of cell proliferation and differentiation. 1alpha,25(OH)2D3 induced epithelial differentiation in SW480-ADH human colon carcinoma cell line by promoting expression of the proteins implicated in adherent junction formation, including E-cadherin, and by inhibiting beta-catenin transcriptional activity. 1alpha,25(OH)2D3 activated the human Id1 gene promoter and rapidly induced Id1 RNA and protein. Ectopic overexpression of Id1 was not sufficient to induce E-cadherin, which was critical for the morphological changes induced by 1alpha,25(OH)2D3 in SW480-ADH cells. Conversely, Id2 transcription rate, RNA and protein levels were decreased by 1alpha,25(OH)2D3. Id2 downregulation by 1alpha,25(OH)2D3 mediated the antiproliferative effect of 1alpha,25(OH)2D3 on SW480-ADH cells. In addition, we showed that 1alpha,25(OH)2D3 changed the levels of the inducer of angiogenesis, vascular endothelial growth factor and the potent antiangiogenic factor thrombospondin-1, leading to a balanced change in the angiogenic potential of SW480-ADH human colon carcinoma cells. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/16007183/1alpha25_Dihydroxyvitamin_D3_regulates_the_expression_of_Id1_and_Id2_genes_and_the_angiogenic_phenotype_of_human_colon_carcinoma_cells_ L2 - https://doi.org/10.1038/sj.onc.1208801 DB - PRIME DP - Unbound Medicine ER -