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Autologous serum skin test reactivity in patients with non-allergic asthma.
Clin Exp Allergy. 2005 Jul; 35(7):849-53.CE

Abstract

BACKGROUND

Inflammatory alterations of respiratory airways have been found in patients with non-allergic asthma, but the triggering event has not been defined. An autoimmune activation of inflammatory cells has been hypothesized.

OBJECTIVE

To evaluate whether histamine-releasing factors are present in sera from non-allergic asthmatics.

METHODS

Twenty-four patients with non-allergic asthma underwent in vivo autologous serum skin test (ASST) and in vitro basophil histamine release assay using autologous basophils as well as basophils from normal donors. Twenty-seven subjects with respiratory allergy and three normal subjects were chosen as control.

RESULTS

ASST was positive in 14/24 non-allergic asthmatics (58%) whereas it was negative in all 30 control subjects (P<0.001). The serum of only one ASST-positive patient out of 12 (8.4%) induced in vitro histamine release from autologous basophils. The serum from another ASST-positive patient induced histamine release from membrane IgE-stripped autologous basophils. Sera from either non-allergic asthmatics or from control subjects did not provoke significant histamine release from basophils from three normal donors.

CONCLUSION

Skin reactivity to autologous serum is common among non-allergic asthmatics, indicating the presence of circulating histamine-releasing factors. However, only in a minority of patients in vitro functional evidence of histamine-releasing autoantibodies (anti-FcepsilonRI or anti-IgE) was obtained. The presence of circulating histamine-releasing factors might contribute to initiation/maintenance of inflammation in respiratory airways of non-allergic asthmatics.

Authors+Show Affiliations

Tedeschi A
Second Division of Internal Medicine, IRCCS Ospedale Maggiore Policlinico, Milano, Italy.
Comi AL
No affiliation info available
Lorini M
No affiliation info available
Tosini C
No affiliation info available
Miadonna A
No affiliation info available

MeSH

AsthmaAutoantibodiesBasophilsFemaleHistamine ReleaseHumansMaleMast CellsMiddle AgedSeverity of Illness IndexSkin Tests

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16008669

Citation

Tedeschi, A, et al. "Autologous Serum Skin Test Reactivity in Patients With Non-allergic Asthma." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 35, no. 7, 2005, pp. 849-53.
Tedeschi A, Comi AL, Lorini M, et al. Autologous serum skin test reactivity in patients with non-allergic asthma. Clin Exp Allergy. 2005;35(7):849-53.
Tedeschi, A., Comi, A. L., Lorini, M., Tosini, C., & Miadonna, A. (2005). Autologous serum skin test reactivity in patients with non-allergic asthma. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 35(7), 849-53.
Tedeschi A, et al. Autologous Serum Skin Test Reactivity in Patients With Non-allergic Asthma. Clin Exp Allergy. 2005;35(7):849-53. PubMed PMID: 16008669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Autologous serum skin test reactivity in patients with non-allergic asthma. AU - Tedeschi,A, AU - Comi,A L, AU - Lorini,M, AU - Tosini,C, AU - Miadonna,A, PY - 2005/7/13/pubmed PY - 2006/1/31/medline PY - 2005/7/13/entrez SP - 849 EP - 53 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 35 IS - 7 N2 - BACKGROUND: Inflammatory alterations of respiratory airways have been found in patients with non-allergic asthma, but the triggering event has not been defined. An autoimmune activation of inflammatory cells has been hypothesized. OBJECTIVE: To evaluate whether histamine-releasing factors are present in sera from non-allergic asthmatics. METHODS: Twenty-four patients with non-allergic asthma underwent in vivo autologous serum skin test (ASST) and in vitro basophil histamine release assay using autologous basophils as well as basophils from normal donors. Twenty-seven subjects with respiratory allergy and three normal subjects were chosen as control. RESULTS: ASST was positive in 14/24 non-allergic asthmatics (58%) whereas it was negative in all 30 control subjects (P<0.001). The serum of only one ASST-positive patient out of 12 (8.4%) induced in vitro histamine release from autologous basophils. The serum from another ASST-positive patient induced histamine release from membrane IgE-stripped autologous basophils. Sera from either non-allergic asthmatics or from control subjects did not provoke significant histamine release from basophils from three normal donors. CONCLUSION: Skin reactivity to autologous serum is common among non-allergic asthmatics, indicating the presence of circulating histamine-releasing factors. However, only in a minority of patients in vitro functional evidence of histamine-releasing autoantibodies (anti-FcepsilonRI or anti-IgE) was obtained. The presence of circulating histamine-releasing factors might contribute to initiation/maintenance of inflammation in respiratory airways of non-allergic asthmatics. SN - 0954-7894 UR - https://www.unboundmedicine.com/medline/citation/16008669/Autologous_serum_skin_test_reactivity_in_patients_with_non_allergic_asthma_ DB - PRIME DP - Unbound Medicine ER -