TY - JOUR T1 - High-performance liquid chromatography method for the quantification of entacapone in human plasma. AU - Ramakrishna,N V S, AU - Vishwottam,K N, AU - Wishu,S, AU - Koteshwara,M, AU - Chidambara,J, PY - 2005/04/28/received PY - 2005/06/16/revised PY - 2005/06/22/accepted PY - 2005/7/13/pubmed PY - 2005/9/22/medline PY - 2005/7/13/entrez SP - 189 EP - 94 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 823 IS - 2 N2 - A simple, sensitive and selective HPLC method with UV detection (315 nm) was developed and validated for quantitation of entacapone in human plasma, the newest addition to the group of antiparkinsonian agents. Following a single-step liquid-liquid extraction (LLE) with ethyl acetate/n-hexane (30/70, v/v), the analyte and internal standard (rofecoxib) were separated using an isocratic mobile phase of 30 mM phosphate buffer (pH 2.75)/acetonitrile (62/38, v/v) on a reverse phase C18 column. The lower limit of quantitation was 25 ng/mL, with a relative standard deviation of less than 8%. A linear range of 25-2500 ng/mL was established. This HPLC method was validated with between-batch and within-batch precision of 2.2-4.2% and 1.7-7.8%, respectively. The between-batch and within-batch accuracy was 98.7-107.5% and 97.5-106.0%, respectively. Frequently coadministered drugs did not interfere with the described methodology. Stability of entacapone in plasma was excellent, with no evidence of degradation during sample processing (autosampler) and 30 days storage in a freezer. This validated method is sensitive, simple and repeatable enough to be used in pharmacokinetic studies. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16009606/High_performance_liquid_chromatography_method_for_the_quantification_of_entacapone_in_human_plasma_ DB - PRIME DP - Unbound Medicine ER -