[Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC].Zhongguo Zhong Yao Za Zhi. 2005 Apr; 30(7):523-5.ZZ
Abstract
OBJECTIVE
To establish a method for determination of galanthamine in Lycoris radiata.
METHOD
HPLC separation was carried on a column of ODS (4.6 mm x 150 mm, 5 microm), with the mobile phase of phosphate buffer (pH = 3-4)-methanol (93:7) at the flow rate of 1.0 mL x min(-1), and the detection wavelength was set at 289 nm.
RESULT
Galanthamine revealed linearity within the range of 3-30 microg x mL(-1) (r = 0.9997), the detection limit was 0.3 ng. The average recovery was 99.5% (RSD = 0.5%).
CONCLUSION
The method is easy to operate and the results of the determination are accurate, it can be used to evaluate the quality of L. radiata.
MeSH
Pub Type(s)
English Abstract
Journal Article
Language
chi
PubMed ID
16011099
Citation
Wu, Fang-li, et al. "[Determination of Galanthamine in Bulb of Lycoris Radiata By RP-HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 30, no. 7, 2005, pp. 523-5.
Wu FL, Li AZ, Mao HF. [Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2005;30(7):523-5.
Wu, F. L., Li, A. Z., & Mao, H. F. (2005). [Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 30(7), 523-5.
Wu FL, Li AZ, Mao HF. [Determination of Galanthamine in Bulb of Lycoris Radiata By RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2005;30(7):523-5. PubMed PMID: 16011099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - [Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC].
AU - Wu,Fang-li,
AU - Li,Ai-zhen,
AU - Mao,Hui-fang,
PY - 2005/7/14/pubmed
PY - 2006/3/3/medline
PY - 2005/7/14/entrez
SP - 523
EP - 5
JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
JO - Zhongguo Zhong Yao Za Zhi
VL - 30
IS - 7
N2 - OBJECTIVE: To establish a method for determination of galanthamine in Lycoris radiata. METHOD: HPLC separation was carried on a column of ODS (4.6 mm x 150 mm, 5 microm), with the mobile phase of phosphate buffer (pH = 3-4)-methanol (93:7) at the flow rate of 1.0 mL x min(-1), and the detection wavelength was set at 289 nm. RESULT: Galanthamine revealed linearity within the range of 3-30 microg x mL(-1) (r = 0.9997), the detection limit was 0.3 ng. The average recovery was 99.5% (RSD = 0.5%). CONCLUSION: The method is easy to operate and the results of the determination are accurate, it can be used to evaluate the quality of L. radiata.
SN - 1001-5302
UR - https://www.unboundmedicine.com/medline/citation/16011099/[Determination_of_galanthamine_in_bulb_of_Lycoris_radiata_by_RP_HPLC]_
DB - PRIME
DP - Unbound Medicine
ER -