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[Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC].
Zhongguo Zhong Yao Za Zhi. 2005 Apr; 30(7):523-5.ZZ

Abstract

OBJECTIVE

To establish a method for determination of galanthamine in Lycoris radiata.

METHOD

HPLC separation was carried on a column of ODS (4.6 mm x 150 mm, 5 microm), with the mobile phase of phosphate buffer (pH = 3-4)-methanol (93:7) at the flow rate of 1.0 mL x min(-1), and the detection wavelength was set at 289 nm.

RESULT

Galanthamine revealed linearity within the range of 3-30 microg x mL(-1) (r = 0.9997), the detection limit was 0.3 ng. The average recovery was 99.5% (RSD = 0.5%).

CONCLUSION

The method is easy to operate and the results of the determination are accurate, it can be used to evaluate the quality of L. radiata.

Authors+Show Affiliations

Zhejiang Yixin Pharmaceutical Co. LTD, Lanxi 321100, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

16011099

Citation

Wu, Fang-li, et al. "[Determination of Galanthamine in Bulb of Lycoris Radiata By RP-HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 30, no. 7, 2005, pp. 523-5.
Wu FL, Li AZ, Mao HF. [Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2005;30(7):523-5.
Wu, F. L., Li, A. Z., & Mao, H. F. (2005). [Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 30(7), 523-5.
Wu FL, Li AZ, Mao HF. [Determination of Galanthamine in Bulb of Lycoris Radiata By RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2005;30(7):523-5. PubMed PMID: 16011099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC]. AU - Wu,Fang-li, AU - Li,Ai-zhen, AU - Mao,Hui-fang, PY - 2005/7/14/pubmed PY - 2006/3/3/medline PY - 2005/7/14/entrez SP - 523 EP - 5 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 30 IS - 7 N2 - OBJECTIVE: To establish a method for determination of galanthamine in Lycoris radiata. METHOD: HPLC separation was carried on a column of ODS (4.6 mm x 150 mm, 5 microm), with the mobile phase of phosphate buffer (pH = 3-4)-methanol (93:7) at the flow rate of 1.0 mL x min(-1), and the detection wavelength was set at 289 nm. RESULT: Galanthamine revealed linearity within the range of 3-30 microg x mL(-1) (r = 0.9997), the detection limit was 0.3 ng. The average recovery was 99.5% (RSD = 0.5%). CONCLUSION: The method is easy to operate and the results of the determination are accurate, it can be used to evaluate the quality of L. radiata. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/16011099/[Determination_of_galanthamine_in_bulb_of_Lycoris_radiata_by_RP_HPLC]_ DB - PRIME DP - Unbound Medicine ER -