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[Determination of flavonoids in buds of Herba Artemisiae Scopariae by HPLC].
Zhongguo Zhong Yao Za Zhi. 2005 Apr; 30(8):591-4.ZZ

Abstract

OBJECTIVE

To develop a quantitative method for the determination of four flavonoids in buds of Herba Artemisiae Scopariae.

METHOD

The sample was extracted by ultrasonic with ethyl acetate for 30 minutes and separated on kromasil ODS column with mobile phase of acetonitrile-tetrahydrofuran-1% acetic acid solution (2.5:21.5:76) and detection wavelength was 347 nm.

RESULT

The arcapillin, cirsiliol, cirsimaritin and cirsilineol in bud extract were well separated by this method. Linearities of arcapillin, cirsiliol, cirsimaritin and cirsilineol were good (r = 0.9992, 0.9999, 0.9997, 0.9997) in ranges of 0.035-0.56, 0.043-0.69, 0.040-0.64 and 0.040-0.64 microg, respectively. The average recoveries were 96.8%, 97.3%, 96.8%, 98.5% and RSD the values of repeatability were 2.5%, 3.0%, 1.5%, 3.2%, respectively.

CONCLUSION

The method validation data indicated that the method is reliable and it can be used for quality control of Herba Artemisiae Scopariae and its preparations.

Authors+Show Affiliations

Lin S
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Zhang QW
No affiliation info available
Zhang NN
No affiliation info available
Zhang YX
No affiliation info available

MeSH

ArtemisiaChromatography, High Pressure LiquidFlavonesFlowersPlants, MedicinalQuality Control

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16011282

Citation

Lin, Sheng, et al. "[Determination of Flavonoids in Buds of Herba Artemisiae Scopariae By HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 30, no. 8, 2005, pp. 591-4.
Lin S, Zhang QW, Zhang NN, et al. [Determination of flavonoids in buds of Herba Artemisiae Scopariae by HPLC]. Zhongguo Zhong Yao Za Zhi. 2005;30(8):591-4.
Lin, S., Zhang, Q. W., Zhang, N. N., & Zhang, Y. X. (2005). [Determination of flavonoids in buds of Herba Artemisiae Scopariae by HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 30(8), 591-4.
Lin S, et al. [Determination of Flavonoids in Buds of Herba Artemisiae Scopariae By HPLC]. Zhongguo Zhong Yao Za Zhi. 2005;30(8):591-4. PubMed PMID: 16011282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of flavonoids in buds of Herba Artemisiae Scopariae by HPLC]. AU - Lin,Sheng, AU - Zhang,Qi-Wei, AU - Zhang,Ning-Ning, AU - Zhang,Yong-Xin, PY - 2005/7/14/pubmed PY - 2006/4/19/medline PY - 2005/7/14/entrez SP - 591 EP - 4 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 30 IS - 8 N2 - OBJECTIVE: To develop a quantitative method for the determination of four flavonoids in buds of Herba Artemisiae Scopariae. METHOD: The sample was extracted by ultrasonic with ethyl acetate for 30 minutes and separated on kromasil ODS column with mobile phase of acetonitrile-tetrahydrofuran-1% acetic acid solution (2.5:21.5:76) and detection wavelength was 347 nm. RESULT: The arcapillin, cirsiliol, cirsimaritin and cirsilineol in bud extract were well separated by this method. Linearities of arcapillin, cirsiliol, cirsimaritin and cirsilineol were good (r = 0.9992, 0.9999, 0.9997, 0.9997) in ranges of 0.035-0.56, 0.043-0.69, 0.040-0.64 and 0.040-0.64 microg, respectively. The average recoveries were 96.8%, 97.3%, 96.8%, 98.5% and RSD the values of repeatability were 2.5%, 3.0%, 1.5%, 3.2%, respectively. CONCLUSION: The method validation data indicated that the method is reliable and it can be used for quality control of Herba Artemisiae Scopariae and its preparations. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/16011282/[Determination_of_flavonoids_in_buds_of_Herba_Artemisiae_Scopariae_by_HPLC]_ DB - PRIME DP - Unbound Medicine ER -