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[Determination of complanatoside A in semen Astragali complanati by HPLC].
Zhongguo Zhong Yao Za Zhi. 2005 Apr; 30(8):600-2.ZZ

Abstract

OBJECTIVE

To establish the determination method for complanatoside A in seeds of Astragalus complanatus.

METHOD

An HPLC method has been developed to separate complanatoside A on ZORBAX EXTEND-C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-water-phosphoric acid (20:80:0.2) as mobile phase and UV detection at 267 nm.

RESULT

The good linearity of complanatoside A ranged 0.086-0.430 microg, r = 0.9999. An average recovery of 99.8% (n = 5) was obtained with a RSD of 1.0%.

CONCLUSION

The established method is proved to be stability, fast, accurate and can be used for quantification of Complanatoside A in Semen Astragali Complanati.

Authors+Show Affiliations

Beijing University of Chinese Traditional Medicine, Beijing 100029, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16011285

Citation

Zhang, Jian-Jun, et al. "[Determination of Complanatoside a in Semen Astragali Complanati By HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 30, no. 8, 2005, pp. 600-2.
Zhang JJ, Yan XL, Zhang YJ, et al. [Determination of complanatoside A in semen Astragali complanati by HPLC]. Zhongguo Zhong Yao Za Zhi. 2005;30(8):600-2.
Zhang, J. J., Yan, X. L., Zhang, Y. J., Wang, Y. P., Zhang, Q. J., Zeng, F. Y., Li, W., & Ou, L. N. (2005). [Determination of complanatoside A in semen Astragali complanati by HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 30(8), 600-2.
Zhang JJ, et al. [Determination of Complanatoside a in Semen Astragali Complanati By HPLC]. Zhongguo Zhong Yao Za Zhi. 2005;30(8):600-2. PubMed PMID: 16011285.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of complanatoside A in semen Astragali complanati by HPLC]. AU - Zhang,Jian-Jun, AU - Yan,Xing-Li, AU - Zhang,Yu-Jie, AU - Wang,Yu-Ping, AU - Zhang,Qiu-Ju, AU - Zeng,Feng-Ying, AU - Li,Wei, AU - Ou,Li-Na, PY - 2005/7/14/pubmed PY - 2006/4/19/medline PY - 2005/7/14/entrez SP - 600 EP - 2 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 30 IS - 8 N2 - OBJECTIVE: To establish the determination method for complanatoside A in seeds of Astragalus complanatus. METHOD: An HPLC method has been developed to separate complanatoside A on ZORBAX EXTEND-C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-water-phosphoric acid (20:80:0.2) as mobile phase and UV detection at 267 nm. RESULT: The good linearity of complanatoside A ranged 0.086-0.430 microg, r = 0.9999. An average recovery of 99.8% (n = 5) was obtained with a RSD of 1.0%. CONCLUSION: The established method is proved to be stability, fast, accurate and can be used for quantification of Complanatoside A in Semen Astragali Complanati. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/16011285/[Determination_of_complanatoside_A_in_semen_Astragali_complanati_by_HPLC]_ DB - PRIME DP - Unbound Medicine ER -