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Vertical distribution of structure and function of the methanogenic archaeal community in Lake Dagow sediment.
Environ Microbiol. 2005 Aug; 7(8):1139-49.EM

Abstract

Detailed studies on the relation of structure and function of microbial communities in a sediment depth profile scarcely exist. We determined as functional aspect the vertical distribution of the acetotrophic and hydrogenotrophic CH4 production activity by measuring production rates and stable 13C/12C-isotopic signatures of CH4 in the profundal sediment of Lake Dagow. The structural aspect was determined by the composition of the methanogenic community by quantifying the abundance of different archaeal groups using 'real-time' polymerase chain reaction and analysis of terminal restriction fragment length polymorphism (T-RFLP). Methane production rates in the surface sediment (0-3 cm depth) were higher in August than in May, but strongly decreased with depth (down to 20 cm). The delta13C of the produced CH4 and CO2 indicated an increase in isotopic fractionation with sediment depth. The relative contribution of hydrogenotrophic to total methanogenesis, which was calculated from the isotopic signatures, increased with depth from about 22% to 38%. Total numbers of microorganisms were higher in August than in May, but strongly decreased with depth. The increase of microorganisms from May to August mainly resulted from Bacteria. The Archaea, on the other hand, exhibited a rather constant abundance, but also decreased with depth from about 1 x 10(8) copies of the archaeal 16S rRNA gene per gram of dry sediment at the surface to 4 x 10(7) copies per gram at 15-20 cm depth. T-RFLP analysis combined with phylogenetic analysis of cloned sequences of the archaeal 16S rRNA genes showed that the methanogenic community consisted mainly of Methanomicrobiales and Methanosaetaceae. The relative abundance of Methanosaetaceae decreased with depth, whereas that of Methanomicrobiales slightly increased. Hence, the vertical distribution of the functional characteristics (CH4 production from acetate versus H2/CO2) was reflected in the structure of the community consisting of acetotrophic (Methanosaetaceae) versus hydrogenotrophic (Methanomicrobiales) phenotypes.

Authors+Show Affiliations

Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch-Str., 35043 Marburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16011751

Citation

Chan, On Chim, et al. "Vertical Distribution of Structure and Function of the Methanogenic Archaeal Community in Lake Dagow Sediment." Environmental Microbiology, vol. 7, no. 8, 2005, pp. 1139-49.
Chan OC, Claus P, Casper P, et al. Vertical distribution of structure and function of the methanogenic archaeal community in Lake Dagow sediment. Environ Microbiol. 2005;7(8):1139-49.
Chan, O. C., Claus, P., Casper, P., Ulrich, A., Lueders, T., & Conrad, R. (2005). Vertical distribution of structure and function of the methanogenic archaeal community in Lake Dagow sediment. Environmental Microbiology, 7(8), 1139-49.
Chan OC, et al. Vertical Distribution of Structure and Function of the Methanogenic Archaeal Community in Lake Dagow Sediment. Environ Microbiol. 2005;7(8):1139-49. PubMed PMID: 16011751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vertical distribution of structure and function of the methanogenic archaeal community in Lake Dagow sediment. AU - Chan,On Chim, AU - Claus,Peter, AU - Casper,Peter, AU - Ulrich,Andreas, AU - Lueders,Tillmann, AU - Conrad,Ralf, PY - 2005/7/14/pubmed PY - 2005/9/15/medline PY - 2005/7/14/entrez SP - 1139 EP - 49 JF - Environmental microbiology JO - Environ Microbiol VL - 7 IS - 8 N2 - Detailed studies on the relation of structure and function of microbial communities in a sediment depth profile scarcely exist. We determined as functional aspect the vertical distribution of the acetotrophic and hydrogenotrophic CH4 production activity by measuring production rates and stable 13C/12C-isotopic signatures of CH4 in the profundal sediment of Lake Dagow. The structural aspect was determined by the composition of the methanogenic community by quantifying the abundance of different archaeal groups using 'real-time' polymerase chain reaction and analysis of terminal restriction fragment length polymorphism (T-RFLP). Methane production rates in the surface sediment (0-3 cm depth) were higher in August than in May, but strongly decreased with depth (down to 20 cm). The delta13C of the produced CH4 and CO2 indicated an increase in isotopic fractionation with sediment depth. The relative contribution of hydrogenotrophic to total methanogenesis, which was calculated from the isotopic signatures, increased with depth from about 22% to 38%. Total numbers of microorganisms were higher in August than in May, but strongly decreased with depth. The increase of microorganisms from May to August mainly resulted from Bacteria. The Archaea, on the other hand, exhibited a rather constant abundance, but also decreased with depth from about 1 x 10(8) copies of the archaeal 16S rRNA gene per gram of dry sediment at the surface to 4 x 10(7) copies per gram at 15-20 cm depth. T-RFLP analysis combined with phylogenetic analysis of cloned sequences of the archaeal 16S rRNA genes showed that the methanogenic community consisted mainly of Methanomicrobiales and Methanosaetaceae. The relative abundance of Methanosaetaceae decreased with depth, whereas that of Methanomicrobiales slightly increased. Hence, the vertical distribution of the functional characteristics (CH4 production from acetate versus H2/CO2) was reflected in the structure of the community consisting of acetotrophic (Methanosaetaceae) versus hydrogenotrophic (Methanomicrobiales) phenotypes. SN - 1462-2912 UR - https://www.unboundmedicine.com/medline/citation/16011751/Vertical_distribution_of_structure_and_function_of_the_methanogenic_archaeal_community_in_Lake_Dagow_sediment_ L2 - https://doi.org/10.1111/j.1462-2920.2005.00790.x DB - PRIME DP - Unbound Medicine ER -