Melioidosis of the spleen diagnosed by 16S rDNA amplification and sequencing.Scand J Infect Dis. 2005; 37(6-7):517-20.SJ
Abstract
We present the case of a 51-y-old male with multiple splenic abscesses that failed to resolve despite antimicrobial therapy targeted at Burkholderia pseudomallei. Elective splenectomy was performed to clarify the diagnosis. Pus from the spleen was subjected to universal bacterial 16S rDNA amplification and sequencing. The sequence obtained was 99% identical to 16S rDNA sequences for Burkholderia pseudomallei. Sequencing of the 16S rDNA is a very promising diagnostic tool allowing quick identification of Burkholderia pseudomallei directly from clinical specimen.
MeSH
Pub Type(s)
Case Reports
Journal Article
Language
eng
PubMed ID
16012017
Citation
Chlebicki, Maciej Piotr, et al. "Melioidosis of the Spleen Diagnosed By 16S rDNA Amplification and Sequencing." Scandinavian Journal of Infectious Diseases, vol. 37, no. 6-7, 2005, pp. 517-20.
Chlebicki MP, Tan BH, Koh TH. Melioidosis of the spleen diagnosed by 16S rDNA amplification and sequencing. Scand J Infect Dis. 2005;37(6-7):517-20.
Chlebicki, M. P., Tan, B. H., & Koh, T. H. (2005). Melioidosis of the spleen diagnosed by 16S rDNA amplification and sequencing. Scandinavian Journal of Infectious Diseases, 37(6-7), 517-20.
Chlebicki MP, Tan BH, Koh TH. Melioidosis of the Spleen Diagnosed By 16S rDNA Amplification and Sequencing. Scand J Infect Dis. 2005;37(6-7):517-20. PubMed PMID: 16012017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Melioidosis of the spleen diagnosed by 16S rDNA amplification and sequencing.
AU - Chlebicki,Maciej Piotr,
AU - Tan,Ban Hock,
AU - Koh,Tse Hsien,
PY - 2005/7/14/pubmed
PY - 2005/9/13/medline
PY - 2005/7/14/entrez
SP - 517
EP - 20
JF - Scandinavian journal of infectious diseases
JO - Scand J Infect Dis
VL - 37
IS - 6-7
N2 - We present the case of a 51-y-old male with multiple splenic abscesses that failed to resolve despite antimicrobial therapy targeted at Burkholderia pseudomallei. Elective splenectomy was performed to clarify the diagnosis. Pus from the spleen was subjected to universal bacterial 16S rDNA amplification and sequencing. The sequence obtained was 99% identical to 16S rDNA sequences for Burkholderia pseudomallei. Sequencing of the 16S rDNA is a very promising diagnostic tool allowing quick identification of Burkholderia pseudomallei directly from clinical specimen.
SN - 0036-5548
UR - https://www.unboundmedicine.com/medline/citation/16012017/Melioidosis_of_the_spleen_diagnosed_by_16S_rDNA_amplification_and_sequencing_
DB - PRIME
DP - Unbound Medicine
ER -