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Melioidosis of the spleen diagnosed by 16S rDNA amplification and sequencing.
Scand J Infect Dis. 2005; 37(6-7):517-20.SJ

Abstract

We present the case of a 51-y-old male with multiple splenic abscesses that failed to resolve despite antimicrobial therapy targeted at Burkholderia pseudomallei. Elective splenectomy was performed to clarify the diagnosis. Pus from the spleen was subjected to universal bacterial 16S rDNA amplification and sequencing. The sequence obtained was 99% identical to 16S rDNA sequences for Burkholderia pseudomallei. Sequencing of the 16S rDNA is a very promising diagnostic tool allowing quick identification of Burkholderia pseudomallei directly from clinical specimen.

Authors+Show Affiliations

Chlebicki MP
Department of Infectious Diseases, Singapore General Hospital, Singapore. pchlebicki@hotmail.com
Tan BH
No affiliation info available
Koh TH
No affiliation info available

MeSH

Amoxicillin-Potassium Clavulanate CombinationAnti-Bacterial AgentsBurkholderia pseudomalleiCeftazidimeHumansMaleMelioidosisMiddle AgedRNA, BacterialRNA, Ribosomal, 16SSplenic Diseases

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

16012017

Citation

Chlebicki, Maciej Piotr, et al. "Melioidosis of the Spleen Diagnosed By 16S rDNA Amplification and Sequencing." Scandinavian Journal of Infectious Diseases, vol. 37, no. 6-7, 2005, pp. 517-20.
Chlebicki MP, Tan BH, Koh TH. Melioidosis of the spleen diagnosed by 16S rDNA amplification and sequencing. Scand J Infect Dis. 2005;37(6-7):517-20.
Chlebicki, M. P., Tan, B. H., & Koh, T. H. (2005). Melioidosis of the spleen diagnosed by 16S rDNA amplification and sequencing. Scandinavian Journal of Infectious Diseases, 37(6-7), 517-20.
Chlebicki MP, Tan BH, Koh TH. Melioidosis of the Spleen Diagnosed By 16S rDNA Amplification and Sequencing. Scand J Infect Dis. 2005;37(6-7):517-20. PubMed PMID: 16012017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Melioidosis of the spleen diagnosed by 16S rDNA amplification and sequencing. AU - Chlebicki,Maciej Piotr, AU - Tan,Ban Hock, AU - Koh,Tse Hsien, PY - 2005/7/14/pubmed PY - 2005/9/13/medline PY - 2005/7/14/entrez SP - 517 EP - 20 JF - Scandinavian journal of infectious diseases JO - Scand J Infect Dis VL - 37 IS - 6-7 N2 - We present the case of a 51-y-old male with multiple splenic abscesses that failed to resolve despite antimicrobial therapy targeted at Burkholderia pseudomallei. Elective splenectomy was performed to clarify the diagnosis. Pus from the spleen was subjected to universal bacterial 16S rDNA amplification and sequencing. The sequence obtained was 99% identical to 16S rDNA sequences for Burkholderia pseudomallei. Sequencing of the 16S rDNA is a very promising diagnostic tool allowing quick identification of Burkholderia pseudomallei directly from clinical specimen. SN - 0036-5548 UR - https://www.unboundmedicine.com/medline/citation/16012017/Melioidosis_of_the_spleen_diagnosed_by_16S_rDNA_amplification_and_sequencing_ DB - PRIME DP - Unbound Medicine ER -