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Anti-myeloperoxidase IgG subclass distribution and avidity in sera from patients with propylthiouracil-induced antineutrophil cytoplasmic antibodies associated vasculitis.
Clin Immunol. 2005 Oct; 117(1):87-93.CI

Abstract

OBJECTIVE

Propylthiouracil (PTU) could induce MPO-ANCA-positive vasculitis. The aim of this study was to compare the IgG subclass distribution and avidity of MPO-ANCA in sera from patients with primary ANCA-associated vasculitis (AASV) and PTU-induced vasculitis.

METHODS

Nineteen patients with primary AASV with MPO-ANCA and thirteen patients with PTU-induced vasculitis were enrolled in the current study. Sera in both active phase and remission were collected. Anti-MPO IgG subclasses were detected by antigen specific ELISAs using specific monoclonal antibodies as second antibodies, and MPO-ANCA avidity was assessed by antigen-inhibition ELISAs.

RESULTS

In primary AASV, all four anti-MPO IgG subclasses could be detected in active phase with IgG1 (100%), IgG2 (73.7%), IgG3 (63.2%) and IgG4 (94.7%), and in remission, IgG1 and IgG4 subclasses in most patients remained positive. However, in PTU-induced vasculitis, anti-MPO IgG3 subclass could not be detected, the anti-MPO IgG subclasses in active phase were IgG1 (100%), IgG2 (61.5%) and IgG4 (46.2%). Furthermore, five out of the six patients (88.8%) with PTU-induced vasculitis with positive IgG4 subclass in active phase turned to negative in remission, however, only eight out of the fourteen patients (57.1%) with primary AASV turned to negative. The median avidity constant of MPO-ANCA was 56 (8.96 to >140) x 10(7) mol/l for patients with primary AASV and 0.7 (<0.28 to >140) x 10(7) mol/l for patients with PTU-induced vasculitis respectively. Furthermore, the relative levels of MPO-ANCA avidity were associated with elevation of ESR in primary AASV and were associated with BVAS scores in patients with PTU-induced vasculitis, respectively.

CONCLUSION

MPO-ANCA IgG subclass distribution and avidity were different between patients with primary AASV and PTU-induced vasculitis. It was suggested that the mechanism of ANCA production in PTU-induced vasculitis was different from that in primary AASV, and the avidity of MPO-ANCA might be associated with disease activity.

Authors+Show Affiliations

Department of Nephrology, Peking University First Hospital, Beijing, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16019262

Citation

Gao, Ying, et al. "Anti-myeloperoxidase IgG Subclass Distribution and Avidity in Sera From Patients With Propylthiouracil-induced Antineutrophil Cytoplasmic Antibodies Associated Vasculitis." Clinical Immunology (Orlando, Fla.), vol. 117, no. 1, 2005, pp. 87-93.
Gao Y, Ye H, Yu F, et al. Anti-myeloperoxidase IgG subclass distribution and avidity in sera from patients with propylthiouracil-induced antineutrophil cytoplasmic antibodies associated vasculitis. Clin Immunol. 2005;117(1):87-93.
Gao, Y., Ye, H., Yu, F., Guo, X. H., & Zhao, M. H. (2005). Anti-myeloperoxidase IgG subclass distribution and avidity in sera from patients with propylthiouracil-induced antineutrophil cytoplasmic antibodies associated vasculitis. Clinical Immunology (Orlando, Fla.), 117(1), 87-93.
Gao Y, et al. Anti-myeloperoxidase IgG Subclass Distribution and Avidity in Sera From Patients With Propylthiouracil-induced Antineutrophil Cytoplasmic Antibodies Associated Vasculitis. Clin Immunol. 2005;117(1):87-93. PubMed PMID: 16019262.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-myeloperoxidase IgG subclass distribution and avidity in sera from patients with propylthiouracil-induced antineutrophil cytoplasmic antibodies associated vasculitis. AU - Gao,Ying, AU - Ye,Hua, AU - Yu,Feng, AU - Guo,Xiao-Hui, AU - Zhao,Ming-Hui, PY - 2005/04/18/received PY - 2005/05/23/revised PY - 2005/06/07/accepted PY - 2005/7/16/pubmed PY - 2005/10/28/medline PY - 2005/7/16/entrez SP - 87 EP - 93 JF - Clinical immunology (Orlando, Fla.) JO - Clin Immunol VL - 117 IS - 1 N2 - OBJECTIVE: Propylthiouracil (PTU) could induce MPO-ANCA-positive vasculitis. The aim of this study was to compare the IgG subclass distribution and avidity of MPO-ANCA in sera from patients with primary ANCA-associated vasculitis (AASV) and PTU-induced vasculitis. METHODS: Nineteen patients with primary AASV with MPO-ANCA and thirteen patients with PTU-induced vasculitis were enrolled in the current study. Sera in both active phase and remission were collected. Anti-MPO IgG subclasses were detected by antigen specific ELISAs using specific monoclonal antibodies as second antibodies, and MPO-ANCA avidity was assessed by antigen-inhibition ELISAs. RESULTS: In primary AASV, all four anti-MPO IgG subclasses could be detected in active phase with IgG1 (100%), IgG2 (73.7%), IgG3 (63.2%) and IgG4 (94.7%), and in remission, IgG1 and IgG4 subclasses in most patients remained positive. However, in PTU-induced vasculitis, anti-MPO IgG3 subclass could not be detected, the anti-MPO IgG subclasses in active phase were IgG1 (100%), IgG2 (61.5%) and IgG4 (46.2%). Furthermore, five out of the six patients (88.8%) with PTU-induced vasculitis with positive IgG4 subclass in active phase turned to negative in remission, however, only eight out of the fourteen patients (57.1%) with primary AASV turned to negative. The median avidity constant of MPO-ANCA was 56 (8.96 to >140) x 10(7) mol/l for patients with primary AASV and 0.7 (<0.28 to >140) x 10(7) mol/l for patients with PTU-induced vasculitis respectively. Furthermore, the relative levels of MPO-ANCA avidity were associated with elevation of ESR in primary AASV and were associated with BVAS scores in patients with PTU-induced vasculitis, respectively. CONCLUSION: MPO-ANCA IgG subclass distribution and avidity were different between patients with primary AASV and PTU-induced vasculitis. It was suggested that the mechanism of ANCA production in PTU-induced vasculitis was different from that in primary AASV, and the avidity of MPO-ANCA might be associated with disease activity. SN - 1521-6616 UR - https://www.unboundmedicine.com/medline/citation/16019262/Anti_myeloperoxidase_IgG_subclass_distribution_and_avidity_in_sera_from_patients_with_propylthiouracil_induced_antineutrophil_cytoplasmic_antibodies_associated_vasculitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1521-6616(05)00180-4 DB - PRIME DP - Unbound Medicine ER -