Tags

Type your tag names separated by a space and hit enter

Endothelial F-actin cytoskeleton in the retinal vasculature of normal and diabetic rats.
Curr Eye Res. 2005 Apr; 30(4):279-90.CE

Abstract

PURPOSE

The purpose of this study was to characterize the endothelial (EC) F-actin cytoskeleton at different orders of the retinal microvasculature in the normal and diabetic rat and determine if changes in F-actin are associated with different stages of diabetes.

METHODS

The EC F-actin cytoskeleton distribution, nuclei shape, and capillary diameter in the retinal vasculature of rats after 5 and 28 weeks of streptozotocin (STZ)-induced diabetes were compared to those in age-matched controls. The eyes were enucleated, arterially perfused, and labeled for F-actin cytoskeleton and nuclei (YO-PRO-1) or microvascular leakage (FITC-dextran). Retinal whole mounts were then examined by confocal microscopy.

RESULTS

The EC F-actin distribution and nuclear size and shape were highly dependent on the location down the vascular tree. The retinal arterial system in the rat shows a high level of F-actin stress fibre (SF) staining. Peripheral border (PB) staining was present in the ECs of all vessels. Diffuse F-actin staining was observed in endothelial cytoplasm in capillaries, venules, and veins. EC nuclei became distinctly less elongated down the vascular tree. In diabetic rats at 5 weeks, at the capillary level the F-actin staining was more diffuse, and areas of F-actin loss were evident. Both dot-like and diffuse leakage was detected in retinal capillaries, and these leakage types were closely associated with the degree of F-actin changes. In diabetic rats at 28 weeks, there was an increased level of SF staining in the arterial system in addition to capillary F-actin changes.

CONCLUSIONS

The EC F-actin cytoskeleton and nuclei shape retinal microvasculature of the normal rat change with location along the vascular tree. In the early stages of diabetes, there are changes to the F-actin cytoskeleton that are clearly associated with microvascular leakage. F-actin distribution could indicate important structural changes in the pathogenesis of diabetic retinopathy.

Authors+Show Affiliations

Yu PK
Centre for Ophthalmology and Visual Science, The University of Western Australia, Nedlands, Western Australia 6009.
Yu DY
No affiliation info available
Cringle SJ
No affiliation info available
Su EN
No affiliation info available

MeSH

ActinsAnimalsBlood-Retinal BarrierCapillariesCapillary PermeabilityCytoskeletonDiabetes Mellitus, ExperimentalDiabetic RetinopathyEndothelium, VascularMaleMicroscopy, ConfocalRatsRats, Sprague-DawleyRetinal Vessels

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16020258

Citation

Yu, Paula K., et al. "Endothelial F-actin Cytoskeleton in the Retinal Vasculature of Normal and Diabetic Rats." Current Eye Research, vol. 30, no. 4, 2005, pp. 279-90.
Yu PK, Yu DY, Cringle SJ, et al. Endothelial F-actin cytoskeleton in the retinal vasculature of normal and diabetic rats. Curr Eye Res. 2005;30(4):279-90.
Yu, P. K., Yu, D. Y., Cringle, S. J., & Su, E. N. (2005). Endothelial F-actin cytoskeleton in the retinal vasculature of normal and diabetic rats. Current Eye Research, 30(4), 279-90.
Yu PK, et al. Endothelial F-actin Cytoskeleton in the Retinal Vasculature of Normal and Diabetic Rats. Curr Eye Res. 2005;30(4):279-90. PubMed PMID: 16020258.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endothelial F-actin cytoskeleton in the retinal vasculature of normal and diabetic rats. AU - Yu,Paula K, AU - Yu,Dao-Yi, AU - Cringle,Stephen J, AU - Su,Er-Ning, PY - 2005/7/16/pubmed PY - 2005/8/3/medline PY - 2005/7/16/entrez SP - 279 EP - 90 JF - Current eye research JO - Curr Eye Res VL - 30 IS - 4 N2 - PURPOSE: The purpose of this study was to characterize the endothelial (EC) F-actin cytoskeleton at different orders of the retinal microvasculature in the normal and diabetic rat and determine if changes in F-actin are associated with different stages of diabetes. METHODS: The EC F-actin cytoskeleton distribution, nuclei shape, and capillary diameter in the retinal vasculature of rats after 5 and 28 weeks of streptozotocin (STZ)-induced diabetes were compared to those in age-matched controls. The eyes were enucleated, arterially perfused, and labeled for F-actin cytoskeleton and nuclei (YO-PRO-1) or microvascular leakage (FITC-dextran). Retinal whole mounts were then examined by confocal microscopy. RESULTS: The EC F-actin distribution and nuclear size and shape were highly dependent on the location down the vascular tree. The retinal arterial system in the rat shows a high level of F-actin stress fibre (SF) staining. Peripheral border (PB) staining was present in the ECs of all vessels. Diffuse F-actin staining was observed in endothelial cytoplasm in capillaries, venules, and veins. EC nuclei became distinctly less elongated down the vascular tree. In diabetic rats at 5 weeks, at the capillary level the F-actin staining was more diffuse, and areas of F-actin loss were evident. Both dot-like and diffuse leakage was detected in retinal capillaries, and these leakage types were closely associated with the degree of F-actin changes. In diabetic rats at 28 weeks, there was an increased level of SF staining in the arterial system in addition to capillary F-actin changes. CONCLUSIONS: The EC F-actin cytoskeleton and nuclei shape retinal microvasculature of the normal rat change with location along the vascular tree. In the early stages of diabetes, there are changes to the F-actin cytoskeleton that are clearly associated with microvascular leakage. F-actin distribution could indicate important structural changes in the pathogenesis of diabetic retinopathy. SN - 0271-3683 UR - https://www.unboundmedicine.com/medline/citation/16020258/Endothelial_F_actin_cytoskeleton_in_the_retinal_vasculature_of_normal_and_diabetic_rats_ DB - PRIME DP - Unbound Medicine ER -