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Development of a liquid chromatography/tandem mass spectrometry assay for the quantification of rabeprazole in human plasma.
Rapid Commun Mass Spectrom. 2005; 19(16):2321-4.RC

Abstract

A simple and sensitive liquid chromatography/tandem mass spectrometry method, employing electrospray ionization, has been developed and validated to quantify rabeprazole in human plasma using omeprazole as the internal standard. The method was validated to demonstrate the specificity, lower limit of quantification, accuracy, and precision of measurements. Selected reaction monitoring was specific for rabeprazole and omeprazole (the internal standard, IS); no endogenous materials interfered with the analysis of rabeprazole and IS from blank plasma. The assay was linear over the concentration range 0.2-200 ng/mL using a 2 microL aliquot of plasma. The correlation coefficients for the calibration curves ranged from 0.9988-0.9994. The intra- and inter-day precision, calculated from quality control samples, were less than 6.65%. A mixture of methanol and water (50:50) was used as the isocratic mobile phase, with 0.1% of formic acid in water, that did not affect the stability of rabeprazole or IS. A simple sample preparation method of protein precipitation with methanol was chosen. The method was employed in a pharmacokinetic study after oral administration of 20 mg rabeprazole to 24 healthy volunteers.

Authors+Show Affiliations

Huang J
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Xu Y
No affiliation info available
Gao S
No affiliation info available
Rui L
No affiliation info available
Guo Q
No affiliation info available

MeSH

2-PyridinylmethylsulfinylbenzimidazolesBenzimidazolesCalibrationCarbohydrate SequenceChromatography, High Pressure LiquidDrug StabilityHumansModels, MolecularMolecular Sequence DataMolecular StructureOmeprazoleRabeprazoleReference StandardsReproducibility of ResultsSensitivity and SpecificitySpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16021618

Citation

Huang, Jinchang, et al. "Development of a Liquid Chromatography/tandem Mass Spectrometry Assay for the Quantification of Rabeprazole in Human Plasma." Rapid Communications in Mass Spectrometry : RCM, vol. 19, no. 16, 2005, pp. 2321-4.
Huang J, Xu Y, Gao S, et al. Development of a liquid chromatography/tandem mass spectrometry assay for the quantification of rabeprazole in human plasma. Rapid Commun Mass Spectrom. 2005;19(16):2321-4.
Huang, J., Xu, Y., Gao, S., Rui, L., & Guo, Q. (2005). Development of a liquid chromatography/tandem mass spectrometry assay for the quantification of rabeprazole in human plasma. Rapid Communications in Mass Spectrometry : RCM, 19(16), 2321-4.
Huang J, et al. Development of a Liquid Chromatography/tandem Mass Spectrometry Assay for the Quantification of Rabeprazole in Human Plasma. Rapid Commun Mass Spectrom. 2005;19(16):2321-4. PubMed PMID: 16021618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a liquid chromatography/tandem mass spectrometry assay for the quantification of rabeprazole in human plasma. AU - Huang,Jinchang, AU - Xu,Yu, AU - Gao,Shu, AU - Rui,Lei, AU - Guo,Qingxiang, PY - 2005/7/16/pubmed PY - 2005/9/24/medline PY - 2005/7/16/entrez SP - 2321 EP - 4 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 19 IS - 16 N2 - A simple and sensitive liquid chromatography/tandem mass spectrometry method, employing electrospray ionization, has been developed and validated to quantify rabeprazole in human plasma using omeprazole as the internal standard. The method was validated to demonstrate the specificity, lower limit of quantification, accuracy, and precision of measurements. Selected reaction monitoring was specific for rabeprazole and omeprazole (the internal standard, IS); no endogenous materials interfered with the analysis of rabeprazole and IS from blank plasma. The assay was linear over the concentration range 0.2-200 ng/mL using a 2 microL aliquot of plasma. The correlation coefficients for the calibration curves ranged from 0.9988-0.9994. The intra- and inter-day precision, calculated from quality control samples, were less than 6.65%. A mixture of methanol and water (50:50) was used as the isocratic mobile phase, with 0.1% of formic acid in water, that did not affect the stability of rabeprazole or IS. A simple sample preparation method of protein precipitation with methanol was chosen. The method was employed in a pharmacokinetic study after oral administration of 20 mg rabeprazole to 24 healthy volunteers. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/16021618/Development_of_a_liquid_chromatography/tandem_mass_spectrometry_assay_for_the_quantification_of_rabeprazole_in_human_plasma_ DB - PRIME DP - Unbound Medicine ER -