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Effects of root replantation and neurotrophic factor treatment on long-term motoneuron survival and axonal regeneration after C7 spinal root avulsion.
Exp Neurol. 2005 Aug; 194(2):341-54.EN

Abstract

In order to determine the effect of nerve root replantation on motoneuron survival and regeneration, we have avulsed and replanted C7 ventral rootlets in adult rabbits under various conditions. Intraspinal alterations and exact positions of ventrolateral replantations were studied in each animal, and the effects of BDNF and/or CNTF administration during replantation investigated in different experimental groups. Six months after lesion, about 70% of motoneurons were lost on the lesioned sides in the C7 segment, without significant differences between groups. Retrograde fluorescent tracing and histological analysis documented that many axons had regrown through the original ventral exit zones or had exited the spinal cord at the lateral replantation site. However, many laterally exiting axons had not grown out directly from the ventral horn through the lateral white matter but had elongated vertically before leaving the spinal cord. The mean axonal diameter was significantly higher in regenerated axons that had exited through the original ventral exit zones in comparison with axons which had grown out laterally. Application of BDNF and/or CNTF did not show any effects on the pathways of regeneration into the replanted root. The results indicate that motoneuron survival cannot be significantly improved by a single dose of neurotrophic factors applied to a ventrolateral replantation site. However, a significant number of myelinating axons are found in replanted roots, and regeneration may be more efficient when outgrowth through the original ventral exit zone is supported.

Authors+Show Affiliations

Department of Plastic- and Handsurgery, Albert-Ludwigs-University, Medical Center, Hugstetterstrasse 55, 79106 Freiburg, Germany. langeva@ch11.ukl.uni-freiburg.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16022862

Citation

Lang, E M., et al. "Effects of Root Replantation and Neurotrophic Factor Treatment On Long-term Motoneuron Survival and Axonal Regeneration After C7 Spinal Root Avulsion." Experimental Neurology, vol. 194, no. 2, 2005, pp. 341-54.
Lang EM, Schlegel N, Sendtner M, et al. Effects of root replantation and neurotrophic factor treatment on long-term motoneuron survival and axonal regeneration after C7 spinal root avulsion. Exp Neurol. 2005;194(2):341-54.
Lang, E. M., Schlegel, N., Sendtner, M., & Asan, E. (2005). Effects of root replantation and neurotrophic factor treatment on long-term motoneuron survival and axonal regeneration after C7 spinal root avulsion. Experimental Neurology, 194(2), 341-54.
Lang EM, et al. Effects of Root Replantation and Neurotrophic Factor Treatment On Long-term Motoneuron Survival and Axonal Regeneration After C7 Spinal Root Avulsion. Exp Neurol. 2005;194(2):341-54. PubMed PMID: 16022862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of root replantation and neurotrophic factor treatment on long-term motoneuron survival and axonal regeneration after C7 spinal root avulsion. AU - Lang,E M, AU - Schlegel,N, AU - Sendtner,M, AU - Asan,E, PY - 2004/10/04/received PY - 2005/02/09/revised PY - 2005/02/14/accepted PY - 2005/7/19/pubmed PY - 2005/9/16/medline PY - 2005/7/19/entrez SP - 341 EP - 54 JF - Experimental neurology JO - Exp. Neurol. VL - 194 IS - 2 N2 - In order to determine the effect of nerve root replantation on motoneuron survival and regeneration, we have avulsed and replanted C7 ventral rootlets in adult rabbits under various conditions. Intraspinal alterations and exact positions of ventrolateral replantations were studied in each animal, and the effects of BDNF and/or CNTF administration during replantation investigated in different experimental groups. Six months after lesion, about 70% of motoneurons were lost on the lesioned sides in the C7 segment, without significant differences between groups. Retrograde fluorescent tracing and histological analysis documented that many axons had regrown through the original ventral exit zones or had exited the spinal cord at the lateral replantation site. However, many laterally exiting axons had not grown out directly from the ventral horn through the lateral white matter but had elongated vertically before leaving the spinal cord. The mean axonal diameter was significantly higher in regenerated axons that had exited through the original ventral exit zones in comparison with axons which had grown out laterally. Application of BDNF and/or CNTF did not show any effects on the pathways of regeneration into the replanted root. The results indicate that motoneuron survival cannot be significantly improved by a single dose of neurotrophic factors applied to a ventrolateral replantation site. However, a significant number of myelinating axons are found in replanted roots, and regeneration may be more efficient when outgrowth through the original ventral exit zone is supported. SN - 0014-4886 UR - https://www.unboundmedicine.com/medline/citation/16022862/Effects_of_root_replantation_and_neurotrophic_factor_treatment_on_long_term_motoneuron_survival_and_axonal_regeneration_after_C7_spinal_root_avulsion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4886(05)00076-2 DB - PRIME DP - Unbound Medicine ER -