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The identification of immunodominant linear epitopes of dengue type 2 virus capsid and NS4a proteins using pin-bound peptides.
Virus Res. 2005 Sep; 112(1-2):60-8.VR

Abstract

We have used multi-pin peptide synthesis strategy to identify B-cell epitopes on two small dengue virus proteins, capsid and NS4a. We have identified several linear, immunodominant epitopes on both these proteins. Almost all these epitopes mapped to regions predicted to be hydrophilic based on Kyte and Doolittle profiles. Of the capsid epitopes identified in this study, the most immunogenic ones mapped to the C-terminal alpha4 helix, which lies on the solvent-exposed surface of the capsid dimer. The capsid epitopes were dengue-specific in that they could recognize antibodies in dengue virus-, but not yellow fever virus (YFV)- or Japanese encephalitis virus (JEV)-immune sera. This study has demonstrated the presence of anti-NS4a antibodies in dengue-patient sera definitively, for the first time, using authentic NS4a-derived pin-bound peptides as capture antigens. All the NS4a epitopes mapped to the amino-terminal third of the NS4a molecule. Our study suggests that the immunodominant epitopes of these two dengue proteins might have the potential to be used as a part of a recombinant multi-epitope protein containing carefully chosen E and NS1 epitopes for the detection of dengue infections with a high degree of sensitivity and specificity.

Authors+Show Affiliations

RGP Laboratory, International Centre for Genetic Engineering and Biotechnology, PO Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16022901

Citation

Anandarao, Ravulapalli, et al. "The Identification of Immunodominant Linear Epitopes of Dengue Type 2 Virus Capsid and NS4a Proteins Using Pin-bound Peptides." Virus Research, vol. 112, no. 1-2, 2005, pp. 60-8.
Anandarao R, Swaminathan S, Khanna N. The identification of immunodominant linear epitopes of dengue type 2 virus capsid and NS4a proteins using pin-bound peptides. Virus Res. 2005;112(1-2):60-8.
Anandarao, R., Swaminathan, S., & Khanna, N. (2005). The identification of immunodominant linear epitopes of dengue type 2 virus capsid and NS4a proteins using pin-bound peptides. Virus Research, 112(1-2), 60-8.
Anandarao R, Swaminathan S, Khanna N. The Identification of Immunodominant Linear Epitopes of Dengue Type 2 Virus Capsid and NS4a Proteins Using Pin-bound Peptides. Virus Res. 2005;112(1-2):60-8. PubMed PMID: 16022901.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The identification of immunodominant linear epitopes of dengue type 2 virus capsid and NS4a proteins using pin-bound peptides. AU - Anandarao,Ravulapalli, AU - Swaminathan,Sathyamangalam, AU - Khanna,Navin, Y1 - 2005/04/26/ PY - 2004/12/06/received PY - 2005/03/08/revised PY - 2005/03/08/accepted PY - 2005/7/19/pubmed PY - 2005/10/4/medline PY - 2005/7/19/entrez SP - 60 EP - 8 JF - Virus research JO - Virus Res VL - 112 IS - 1-2 N2 - We have used multi-pin peptide synthesis strategy to identify B-cell epitopes on two small dengue virus proteins, capsid and NS4a. We have identified several linear, immunodominant epitopes on both these proteins. Almost all these epitopes mapped to regions predicted to be hydrophilic based on Kyte and Doolittle profiles. Of the capsid epitopes identified in this study, the most immunogenic ones mapped to the C-terminal alpha4 helix, which lies on the solvent-exposed surface of the capsid dimer. The capsid epitopes were dengue-specific in that they could recognize antibodies in dengue virus-, but not yellow fever virus (YFV)- or Japanese encephalitis virus (JEV)-immune sera. This study has demonstrated the presence of anti-NS4a antibodies in dengue-patient sera definitively, for the first time, using authentic NS4a-derived pin-bound peptides as capture antigens. All the NS4a epitopes mapped to the amino-terminal third of the NS4a molecule. Our study suggests that the immunodominant epitopes of these two dengue proteins might have the potential to be used as a part of a recombinant multi-epitope protein containing carefully chosen E and NS1 epitopes for the detection of dengue infections with a high degree of sensitivity and specificity. SN - 0168-1702 UR - https://www.unboundmedicine.com/medline/citation/16022901/The_identification_of_immunodominant_linear_epitopes_of_dengue_type_2_virus_capsid_and_NS4a_proteins_using_pin_bound_peptides_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(05)00088-2 DB - PRIME DP - Unbound Medicine ER -