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Attachment of gold nanoparticles to glassy carbon electrode and its application for the direct electrochemistry and electrocatalytic behavior of hemoglobin.
Biosens Bioelectron. 2005 Aug 15; 21(2):337-45.BB

Abstract

Gold nanoparticles have been attached onto glassy carbon electrode surface through sulfhydryl-terminated monolayer and characterized by X-ray photoelectron spectroscopy, atomic force microscopy, electrochemical impedance spectroscopy and cyclic voltammetry. The gold nanoparticles-attached glassy carbon electrodes have been applied to the immobilization/adsorption of hemoglobin, with a monolayer surface coverage of about 2.1 x 10(-10) mol cm(-2), and consequently obtained the direct electrochemistry of hemoglobin. Gold nanoparticles, acting as a bridge of electron transfer, can greatly promote the direct electron transfer between hemoglobin and the modified glassy carbon electrode without the aid of any electron mediator. In phosphate buffer solution with pH 6.8, hemoglobin shows a pair of well-defined redox waves with formal potential (E0') of about -0.085 V (versus Ag/AgCl/saturated KCl). The immobilized hemoglobin maintained its biological activity, showing a surface controlled electrode process with the apparent heterogeneous electron transfer rate constant (ks) of 1.05 s(-1) and charge-transfer coefficient (a) of 0.46, and displays the features of a peroxidase in the electrocatalytic reduction of hydrogen peroxide. A potential application of the hemoglobin-immobilized gold nanoparticles modified glassy carbon electrode as a biosensor to monitor hydrogen peroxide has been investigated. The steady-state current response increases linearly with hydrogen peroxide concentration from 2.0 x 10(-6) to 2.4 x 10(-4) M. The detection limit (3sigma) for hydrogen peroxide is 9.1 x 10(-7) M.

Authors+Show Affiliations

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16023961

Citation

Zhang, Lei, et al. "Attachment of Gold Nanoparticles to Glassy Carbon Electrode and Its Application for the Direct Electrochemistry and Electrocatalytic Behavior of Hemoglobin." Biosensors & Bioelectronics, vol. 21, no. 2, 2005, pp. 337-45.
Zhang L, Jiang X, Wang E, et al. Attachment of gold nanoparticles to glassy carbon electrode and its application for the direct electrochemistry and electrocatalytic behavior of hemoglobin. Biosens Bioelectron. 2005;21(2):337-45.
Zhang, L., Jiang, X., Wang, E., & Dong, S. (2005). Attachment of gold nanoparticles to glassy carbon electrode and its application for the direct electrochemistry and electrocatalytic behavior of hemoglobin. Biosensors & Bioelectronics, 21(2), 337-45.
Zhang L, et al. Attachment of Gold Nanoparticles to Glassy Carbon Electrode and Its Application for the Direct Electrochemistry and Electrocatalytic Behavior of Hemoglobin. Biosens Bioelectron. 2005 Aug 15;21(2):337-45. PubMed PMID: 16023961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Attachment of gold nanoparticles to glassy carbon electrode and its application for the direct electrochemistry and electrocatalytic behavior of hemoglobin. AU - Zhang,Lei, AU - Jiang,Xiue, AU - Wang,Erkang, AU - Dong,Shaojun, Y1 - 2004/12/08/ PY - 2004/08/17/received PY - 2004/10/08/revised PY - 2004/10/11/accepted PY - 2005/7/19/pubmed PY - 2006/2/16/medline PY - 2005/7/19/entrez SP - 337 EP - 45 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 21 IS - 2 N2 - Gold nanoparticles have been attached onto glassy carbon electrode surface through sulfhydryl-terminated monolayer and characterized by X-ray photoelectron spectroscopy, atomic force microscopy, electrochemical impedance spectroscopy and cyclic voltammetry. The gold nanoparticles-attached glassy carbon electrodes have been applied to the immobilization/adsorption of hemoglobin, with a monolayer surface coverage of about 2.1 x 10(-10) mol cm(-2), and consequently obtained the direct electrochemistry of hemoglobin. Gold nanoparticles, acting as a bridge of electron transfer, can greatly promote the direct electron transfer between hemoglobin and the modified glassy carbon electrode without the aid of any electron mediator. In phosphate buffer solution with pH 6.8, hemoglobin shows a pair of well-defined redox waves with formal potential (E0') of about -0.085 V (versus Ag/AgCl/saturated KCl). The immobilized hemoglobin maintained its biological activity, showing a surface controlled electrode process with the apparent heterogeneous electron transfer rate constant (ks) of 1.05 s(-1) and charge-transfer coefficient (a) of 0.46, and displays the features of a peroxidase in the electrocatalytic reduction of hydrogen peroxide. A potential application of the hemoglobin-immobilized gold nanoparticles modified glassy carbon electrode as a biosensor to monitor hydrogen peroxide has been investigated. The steady-state current response increases linearly with hydrogen peroxide concentration from 2.0 x 10(-6) to 2.4 x 10(-4) M. The detection limit (3sigma) for hydrogen peroxide is 9.1 x 10(-7) M. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16023961/Attachment_of_gold_nanoparticles_to_glassy_carbon_electrode_and_its_application_for_the_direct_electrochemistry_and_electrocatalytic_behavior_of_hemoglobin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(04)00515-9 DB - PRIME DP - Unbound Medicine ER -