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Developmental expression of GABA transporter-1 and 3 during formation of the GABAergic synapses in the mouse cerebellar cortex.
Brain Res Dev Brain Res 2005; 158(1-2):41-9BR

Abstract

In the brain, gamma-amino butyric acid (GABA), released extrasynaptically and synaptically from GABAergic neurons, plays important roles in morphogenesis, expression of higher functions and so on. In the GABAergic transmission system, plasma membrane GABA transporters (GATs) mediate GABA-uptake from the synaptic cleft in the mature brain and are thought to mediate diacrine of cytosolic GABA in the immature brain. In the present study, we focused on two GATs (GAT-1 and GAT-3) in the mouse cerebellar cortex, which are widely localized in neural and glial cells. Firstly, we examined the localization of GATs in the dendrites and cell bodies of developing GABAergic neurons, where GABA is extrasynaptically distributed, to clarify the GABA-diacrine before synaptogenesis. Secondly, we examined the developmental changes in the localization of GATs to reveal the development of the GABA-uptake system. Neither transporter was detected within the dendrites and cell bodies of GABAergic neurons, including Purkinje, stellate, basket and Golgi cells, in the immature cerebellar cortex. GAT-1 was observed within the Golgi cell axon terminals after postnatal day 5 (P5) and presynaptic axons of stellate and basket cells after P7. GAT-3 was localized within the astrocyte processes, sealing the GABAergic synapses in the Purkinje cell and granular layers after P10. These results indicated that GABA-diacrine did not work in the mouse cerebellar cortex. The onset of GAT-1-expression was prior to that of GAT-3. GAT-1 started to be localized within the GABAergic axon terminals during synapse formation. GAT-3 started to be localized within astrocyte processes when they sealed the synapses.

Authors+Show Affiliations

Department of Molecular Neuroanatomy, Hokkaido University School of Medicine, Kita-15 Nishi-7, Kita-Ku, Sapporo 060-8638, Japan. takachan@med.hokudai.ac.jpNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16024093

Citation

Takayama, Chitoshi, and Yoshiro Inoue. "Developmental Expression of GABA Transporter-1 and 3 During Formation of the GABAergic Synapses in the Mouse Cerebellar Cortex." Brain Research. Developmental Brain Research, vol. 158, no. 1-2, 2005, pp. 41-9.
Takayama C, Inoue Y. Developmental expression of GABA transporter-1 and 3 during formation of the GABAergic synapses in the mouse cerebellar cortex. Brain Res Dev Brain Res. 2005;158(1-2):41-9.
Takayama, C., & Inoue, Y. (2005). Developmental expression of GABA transporter-1 and 3 during formation of the GABAergic synapses in the mouse cerebellar cortex. Brain Research. Developmental Brain Research, 158(1-2), pp. 41-9.
Takayama C, Inoue Y. Developmental Expression of GABA Transporter-1 and 3 During Formation of the GABAergic Synapses in the Mouse Cerebellar Cortex. Brain Res Dev Brain Res. 2005 Aug 8;158(1-2):41-9. PubMed PMID: 16024093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental expression of GABA transporter-1 and 3 during formation of the GABAergic synapses in the mouse cerebellar cortex. AU - Takayama,Chitoshi, AU - Inoue,Yoshiro, PY - 2005/02/23/received PY - 2005/05/26/revised PY - 2005/05/26/accepted PY - 2005/7/19/pubmed PY - 2005/10/28/medline PY - 2005/7/19/entrez SP - 41 EP - 9 JF - Brain research. Developmental brain research JO - Brain Res. Dev. Brain Res. VL - 158 IS - 1-2 N2 - In the brain, gamma-amino butyric acid (GABA), released extrasynaptically and synaptically from GABAergic neurons, plays important roles in morphogenesis, expression of higher functions and so on. In the GABAergic transmission system, plasma membrane GABA transporters (GATs) mediate GABA-uptake from the synaptic cleft in the mature brain and are thought to mediate diacrine of cytosolic GABA in the immature brain. In the present study, we focused on two GATs (GAT-1 and GAT-3) in the mouse cerebellar cortex, which are widely localized in neural and glial cells. Firstly, we examined the localization of GATs in the dendrites and cell bodies of developing GABAergic neurons, where GABA is extrasynaptically distributed, to clarify the GABA-diacrine before synaptogenesis. Secondly, we examined the developmental changes in the localization of GATs to reveal the development of the GABA-uptake system. Neither transporter was detected within the dendrites and cell bodies of GABAergic neurons, including Purkinje, stellate, basket and Golgi cells, in the immature cerebellar cortex. GAT-1 was observed within the Golgi cell axon terminals after postnatal day 5 (P5) and presynaptic axons of stellate and basket cells after P7. GAT-3 was localized within the astrocyte processes, sealing the GABAergic synapses in the Purkinje cell and granular layers after P10. These results indicated that GABA-diacrine did not work in the mouse cerebellar cortex. The onset of GAT-1-expression was prior to that of GAT-3. GAT-1 started to be localized within the GABAergic axon terminals during synapse formation. GAT-3 started to be localized within astrocyte processes when they sealed the synapses. SN - 0165-3806 UR - https://www.unboundmedicine.com/medline/citation/16024093/Developmental_expression_of_GABA_transporter_1_and_3_during_formation_of_the_GABAergic_synapses_in_the_mouse_cerebellar_cortex_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-3806(05)00167-7 DB - PRIME DP - Unbound Medicine ER -