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Layer-by-layer self-assembled multilayer films of carbon nanotubes and platinum nanoparticles with polyelectrolyte for the fabrication of biosensors.
Biomaterials. 2006 Jan; 27(2):246-55.B

Abstract

Platinum nanoparticle-doped chitosan (CHIT) solution can be easily prepared by treating the CHIT solution with aqueous H2PtCl6 solution followed by chemical reduction of Pt(IV) with NaBH4. Multiwalled carbon nanotubes (MWCNT) are then dispersed in the nanoparticle-doped solution. The resulting Pt-CNT-CHIT material brings new capabilities for electrochemical devices by using the synergistic action of Pt nanaoparticles and CNT. Positively charged Pt-CNT-CHIT solution and negatively charged poly(sodium-p-styrenesulfonate) salt (PSS) have been employed to fabricate stable ultrathin multilayer films on gold electrode and quartz glass slides in a layer-by-layer fashion. Cyclic voltammetric and UV-vis adsorption spectroscopy confirms the consecutive growth of the multilayer films. The modified gold electrode allows low-potential detection of hydrogen peroxide with high sensitivity and fast response time. With the immobilization of cholesterol oxidase onto the electrode surface using glutaric dialdehyde, a biosensor that responds sensitively to cholesterol has been constructed. In pH 6.98 phosphate buffer, almost interference free determination of cholesterol has been realized at 0.1 V vs. SCE with a linear range from 0.01 to 3mM and response time<30s. With the immobilization of another cholesterol esterase enzyme layer, the biosensor was used to determine total cholesterol in serum samples with satisfactory results.

Authors+Show Affiliations

Chemistry and Chemical Engineering College, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Hunan, Changsha 410082, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16026820

Citation

Yang, Minghui, et al. "Layer-by-layer Self-assembled Multilayer Films of Carbon Nanotubes and Platinum Nanoparticles With Polyelectrolyte for the Fabrication of Biosensors." Biomaterials, vol. 27, no. 2, 2006, pp. 246-55.
Yang M, Yang Y, Yang H, et al. Layer-by-layer self-assembled multilayer films of carbon nanotubes and platinum nanoparticles with polyelectrolyte for the fabrication of biosensors. Biomaterials. 2006;27(2):246-55.
Yang, M., Yang, Y., Yang, H., Shen, G., & Yu, R. (2006). Layer-by-layer self-assembled multilayer films of carbon nanotubes and platinum nanoparticles with polyelectrolyte for the fabrication of biosensors. Biomaterials, 27(2), 246-55.
Yang M, et al. Layer-by-layer Self-assembled Multilayer Films of Carbon Nanotubes and Platinum Nanoparticles With Polyelectrolyte for the Fabrication of Biosensors. Biomaterials. 2006;27(2):246-55. PubMed PMID: 16026820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Layer-by-layer self-assembled multilayer films of carbon nanotubes and platinum nanoparticles with polyelectrolyte for the fabrication of biosensors. AU - Yang,Minghui, AU - Yang,Yu, AU - Yang,Haifeng, AU - Shen,Guoli, AU - Yu,Ruqin, PY - 2005/03/22/received PY - 2005/05/27/accepted PY - 2005/7/20/pubmed PY - 2005/11/9/medline PY - 2005/7/20/entrez SP - 246 EP - 55 JF - Biomaterials JO - Biomaterials VL - 27 IS - 2 N2 - Platinum nanoparticle-doped chitosan (CHIT) solution can be easily prepared by treating the CHIT solution with aqueous H2PtCl6 solution followed by chemical reduction of Pt(IV) with NaBH4. Multiwalled carbon nanotubes (MWCNT) are then dispersed in the nanoparticle-doped solution. The resulting Pt-CNT-CHIT material brings new capabilities for electrochemical devices by using the synergistic action of Pt nanaoparticles and CNT. Positively charged Pt-CNT-CHIT solution and negatively charged poly(sodium-p-styrenesulfonate) salt (PSS) have been employed to fabricate stable ultrathin multilayer films on gold electrode and quartz glass slides in a layer-by-layer fashion. Cyclic voltammetric and UV-vis adsorption spectroscopy confirms the consecutive growth of the multilayer films. The modified gold electrode allows low-potential detection of hydrogen peroxide with high sensitivity and fast response time. With the immobilization of cholesterol oxidase onto the electrode surface using glutaric dialdehyde, a biosensor that responds sensitively to cholesterol has been constructed. In pH 6.98 phosphate buffer, almost interference free determination of cholesterol has been realized at 0.1 V vs. SCE with a linear range from 0.01 to 3mM and response time<30s. With the immobilization of another cholesterol esterase enzyme layer, the biosensor was used to determine total cholesterol in serum samples with satisfactory results. SN - 0142-9612 UR - https://www.unboundmedicine.com/medline/citation/16026820/Layer_by_layer_self_assembled_multilayer_films_of_carbon_nanotubes_and_platinum_nanoparticles_with_polyelectrolyte_for_the_fabrication_of_biosensors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0142-9612(05)00491-6 DB - PRIME DP - Unbound Medicine ER -