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C-reactive protein production in term human placental tissue.
Placenta 2006 Jun-Jul; 27(6-7):619-25P

Abstract

OBJECTIVE

C-reactive protein (CRP) is a marker of systemic inflammation. Recently, it has been shown that CRP is present in amniotic fluid and fetal urine, and that elevated levels are associated with adverse pregnancy outcome. However, the precise source of amniotic fluid CRP, its regulation, and function during pregnancy is still a matter of debate. The present in vivo and in vitro studies were designed to investigate the production of CRP in human placental tissues.

MATERIAL AND METHODS

Ten paired blood samples from peripheral maternal vein (MV), umbilical cord artery (UA) and umbilical vein (UV) were collected from women with elective caesarean sections at term. The placental protein accumulation capacity of hCG, hPL, leptin and CRP was compared with the dual in vitro perfusion method of an isolated cotyledon of human term placentae and quantified by ELISA. Values for accumulation (release) were calculated as total accumulation of maternal and fetal circuits normalized for tissue weight and duration of perfusion. For gene expression, RNA was extracted from placental tissue and reverse transcribed. RT-PCR and real-time PCR were performed using specific primers.

RESULTS

The median (range) CRP level was significantly different between UA and UV [50.1 ng/ml (12.1-684.6) vs. 61 ng/ml (16.9-708.1)]. The median (range) difference between UV and UA was 9.3 ng/ml (2.2-31.6). A significant correlation was found between MV CRP and both UA and UV CRP levels. Median (range) MV CRP levels [2649 ng/ml (260.1-8299)] were 61.2 (6.5-96.8) fold higher than in the fetus. In vitro, the total accumulation rates (mean+/-SD) were 31+/-13 (mU/g/min, hCG), 1.16+/-0.19 (microg/g/min, hPL), 4.71+/-1.91 (ng/g/min, CRP), and 259+/-118 (pg/g/min, leptin). mRNA for hCG, hPL and leptin was detectable using conventional RT-PCR, while CRP mRNA could only be demonstrated by applying real-time RT-PCR. In the perfused tissue the transcript levels for the four proteins were comparable to those detected in the native control tissue.

CONCLUSIONS

Our results demonstrate that the human placenta produces and releases CRP mainly into the maternal circulation similarly to other analyzed placental proteins under in vitro conditions. Further studies are needed to explore the exact role of placental CRP during pregnancy.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, University of Berne, Inselspital, KKL G3-825, 3010 Berne, BE, Switzerland. antoine.malek@dkf.unibe.chNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16026834

Citation

Malek, A, et al. "C-reactive Protein Production in Term Human Placental Tissue." Placenta, vol. 27, no. 6-7, 2006, pp. 619-25.
Malek A, Bersinger NA, Di Santo S, et al. C-reactive protein production in term human placental tissue. Placenta. 2006;27(6-7):619-25.
Malek, A., Bersinger, N. A., Di Santo, S., Mueller, M. D., Sager, R., Schneider, H., ... Raio, L. (2006). C-reactive protein production in term human placental tissue. Placenta, 27(6-7), pp. 619-25.
Malek A, et al. C-reactive Protein Production in Term Human Placental Tissue. Placenta. 2006;27(6-7):619-25. PubMed PMID: 16026834.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - C-reactive protein production in term human placental tissue. AU - Malek,A, AU - Bersinger,N A, AU - Di Santo,S, AU - Mueller,M D, AU - Sager,R, AU - Schneider,H, AU - Ghezzi,F, AU - Karousou,E, AU - Passi,A, AU - De Luca,G, AU - Raio,L, Y1 - 2005/07/18/ PY - 2005/01/23/received PY - 2005/05/11/revised PY - 2005/05/12/accepted PY - 2005/7/20/pubmed PY - 2006/6/23/medline PY - 2005/7/20/entrez SP - 619 EP - 25 JF - Placenta JO - Placenta VL - 27 IS - 6-7 N2 - OBJECTIVE: C-reactive protein (CRP) is a marker of systemic inflammation. Recently, it has been shown that CRP is present in amniotic fluid and fetal urine, and that elevated levels are associated with adverse pregnancy outcome. However, the precise source of amniotic fluid CRP, its regulation, and function during pregnancy is still a matter of debate. The present in vivo and in vitro studies were designed to investigate the production of CRP in human placental tissues. MATERIAL AND METHODS: Ten paired blood samples from peripheral maternal vein (MV), umbilical cord artery (UA) and umbilical vein (UV) were collected from women with elective caesarean sections at term. The placental protein accumulation capacity of hCG, hPL, leptin and CRP was compared with the dual in vitro perfusion method of an isolated cotyledon of human term placentae and quantified by ELISA. Values for accumulation (release) were calculated as total accumulation of maternal and fetal circuits normalized for tissue weight and duration of perfusion. For gene expression, RNA was extracted from placental tissue and reverse transcribed. RT-PCR and real-time PCR were performed using specific primers. RESULTS: The median (range) CRP level was significantly different between UA and UV [50.1 ng/ml (12.1-684.6) vs. 61 ng/ml (16.9-708.1)]. The median (range) difference between UV and UA was 9.3 ng/ml (2.2-31.6). A significant correlation was found between MV CRP and both UA and UV CRP levels. Median (range) MV CRP levels [2649 ng/ml (260.1-8299)] were 61.2 (6.5-96.8) fold higher than in the fetus. In vitro, the total accumulation rates (mean+/-SD) were 31+/-13 (mU/g/min, hCG), 1.16+/-0.19 (microg/g/min, hPL), 4.71+/-1.91 (ng/g/min, CRP), and 259+/-118 (pg/g/min, leptin). mRNA for hCG, hPL and leptin was detectable using conventional RT-PCR, while CRP mRNA could only be demonstrated by applying real-time RT-PCR. In the perfused tissue the transcript levels for the four proteins were comparable to those detected in the native control tissue. CONCLUSIONS: Our results demonstrate that the human placenta produces and releases CRP mainly into the maternal circulation similarly to other analyzed placental proteins under in vitro conditions. Further studies are needed to explore the exact role of placental CRP during pregnancy. SN - 0143-4004 UR - https://www.unboundmedicine.com/medline/citation/16026834/C_reactive_protein_production_in_term_human_placental_tissue_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4004(05)00160-8 DB - PRIME DP - Unbound Medicine ER -