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Cellular defense against heat shock-induced oxidative damage by mitochondrial NADP+ -dependent isocitrate dehydrogenase.
Free Radic Res. 2005 Apr; 39(4):441-8.FR

Abstract

Heat shock may increase oxidative stress due to increased production of reactive oxygen species and/or the promotion of cellular oxidation events. Mitochondrial NADP+ -dependent isocitrate dehydrogenase (IDPm) produces NADPH, an essential reducing equivalent for the antioxidant system. The protective role of IDPm against heat shock in HEK293 cells, an embryonic kidney cell line, was investigated in control and cells transfected with the cDNA for IDPm, where IDPm activity was 6-7 fold higher than that in the control cells carrying the vector alone. Upon exposure to heat shock, the viability was lower and the protein oxidation, lipid peroxidation and oxidative DNA damage were higher in control cells as compared to HEK293 cells in which IDPm was over-expressed. We also observed the significant difference in the cellular redox status reflected by the endogenous production of reactive oxygen species, NADPH pool and GSH recycling between two cells. The results suggest that IDPm plays an important role as an antioxidant defense enzyme in cellular defense against heat shock through the removal of reactive oxygen species.

Authors+Show Affiliations

Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu 702-701, South Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16028369

Citation

Kim, Hyun Jeong, et al. "Cellular Defense Against Heat Shock-induced Oxidative Damage By Mitochondrial NADP+ -dependent Isocitrate Dehydrogenase." Free Radical Research, vol. 39, no. 4, 2005, pp. 441-8.
Kim HJ, Kang BS, Park JW. Cellular defense against heat shock-induced oxidative damage by mitochondrial NADP+ -dependent isocitrate dehydrogenase. Free Radic Res. 2005;39(4):441-8.
Kim, H. J., Kang, B. S., & Park, J. W. (2005). Cellular defense against heat shock-induced oxidative damage by mitochondrial NADP+ -dependent isocitrate dehydrogenase. Free Radical Research, 39(4), 441-8.
Kim HJ, Kang BS, Park JW. Cellular Defense Against Heat Shock-induced Oxidative Damage By Mitochondrial NADP+ -dependent Isocitrate Dehydrogenase. Free Radic Res. 2005;39(4):441-8. PubMed PMID: 16028369.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cellular defense against heat shock-induced oxidative damage by mitochondrial NADP+ -dependent isocitrate dehydrogenase. AU - Kim,Hyun Jeong, AU - Kang,Beom Sik, AU - Park,Jeen-Woo, PY - 2005/7/21/pubmed PY - 2005/8/12/medline PY - 2005/7/21/entrez SP - 441 EP - 8 JF - Free radical research JO - Free Radic Res VL - 39 IS - 4 N2 - Heat shock may increase oxidative stress due to increased production of reactive oxygen species and/or the promotion of cellular oxidation events. Mitochondrial NADP+ -dependent isocitrate dehydrogenase (IDPm) produces NADPH, an essential reducing equivalent for the antioxidant system. The protective role of IDPm against heat shock in HEK293 cells, an embryonic kidney cell line, was investigated in control and cells transfected with the cDNA for IDPm, where IDPm activity was 6-7 fold higher than that in the control cells carrying the vector alone. Upon exposure to heat shock, the viability was lower and the protein oxidation, lipid peroxidation and oxidative DNA damage were higher in control cells as compared to HEK293 cells in which IDPm was over-expressed. We also observed the significant difference in the cellular redox status reflected by the endogenous production of reactive oxygen species, NADPH pool and GSH recycling between two cells. The results suggest that IDPm plays an important role as an antioxidant defense enzyme in cellular defense against heat shock through the removal of reactive oxygen species. SN - 1071-5762 UR - https://www.unboundmedicine.com/medline/citation/16028369/Cellular_defense_against_heat_shock_induced_oxidative_damage_by_mitochondrial_NADP+__dependent_isocitrate_dehydrogenase_ L2 - https://www.tandfonline.com/doi/full/10.1080/10715760500066265 DB - PRIME DP - Unbound Medicine ER -