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Identification of ellagic acid conjugates and other polyphenolics in muscadine grapes by HPLC-ESI-MS.
J Agric Food Chem. 2005 Jul 27; 53(15):6003-10.JA

Abstract

Ellagic acid, ellagic acid glycosides, and ellagitannins found in various fruits and nuts, including muscadine grape, are reported to have potential health-promoting benefits and antioxidant properties. This study isolated and identified several ellagic acid derivatives present in muscadine grapes and determined their relative antioxidant properties (AOX). Compounds were extracted from grape skins and pulp using methanol, and the solvent was evaporated. Isolates were dissolved in citric acid buffer (pH 3.5) and absorbed onto C18 cartridges. Nonretained polyphenolics were collected separately and again partitioned from Sephadex LH-20, whereas retained polyphenolics were first eluted with ethyl acetate followed by methanol. Ellagic acid derivatives were identified on the basis of UV and mass spectra, and the presence of ellagitannins was confirmed by a significant increase in free ellagic acid with HPLC followed by acid hydrolysis. Muscadine grapes contained phenolic acids, flavonols, anthocyanins, ellagic acid, and numerous ellagic acid derivatives. AOX varied in the order ethyl acetate > methanol > C18 nonretained fractions; each correlated to both total phenolics (r = 0.90) and total ellagic acid (r = 0.99) contents. Results of this study revealed previously unidentified ellagic acid derivatives in muscadine grapes.

Authors+Show Affiliations

Department of Food Science and Human Nutrition, Institute of Food and Agricultural Sciences, University of Florida, P.O. Box 110370, Gainesville, Florida 32611, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16028988

Citation

Lee, Joon-Hee, et al. "Identification of Ellagic Acid Conjugates and Other Polyphenolics in Muscadine Grapes By HPLC-ESI-MS." Journal of Agricultural and Food Chemistry, vol. 53, no. 15, 2005, pp. 6003-10.
Lee JH, Johnson JV, Talcott ST. Identification of ellagic acid conjugates and other polyphenolics in muscadine grapes by HPLC-ESI-MS. J Agric Food Chem. 2005;53(15):6003-10.
Lee, J. H., Johnson, J. V., & Talcott, S. T. (2005). Identification of ellagic acid conjugates and other polyphenolics in muscadine grapes by HPLC-ESI-MS. Journal of Agricultural and Food Chemistry, 53(15), 6003-10.
Lee JH, Johnson JV, Talcott ST. Identification of Ellagic Acid Conjugates and Other Polyphenolics in Muscadine Grapes By HPLC-ESI-MS. J Agric Food Chem. 2005 Jul 27;53(15):6003-10. PubMed PMID: 16028988.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of ellagic acid conjugates and other polyphenolics in muscadine grapes by HPLC-ESI-MS. AU - Lee,Joon-Hee, AU - Johnson,Jodie V, AU - Talcott,Stephen T, PY - 2005/7/21/pubmed PY - 2005/9/2/medline PY - 2005/7/21/entrez SP - 6003 EP - 10 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 53 IS - 15 N2 - Ellagic acid, ellagic acid glycosides, and ellagitannins found in various fruits and nuts, including muscadine grape, are reported to have potential health-promoting benefits and antioxidant properties. This study isolated and identified several ellagic acid derivatives present in muscadine grapes and determined their relative antioxidant properties (AOX). Compounds were extracted from grape skins and pulp using methanol, and the solvent was evaporated. Isolates were dissolved in citric acid buffer (pH 3.5) and absorbed onto C18 cartridges. Nonretained polyphenolics were collected separately and again partitioned from Sephadex LH-20, whereas retained polyphenolics were first eluted with ethyl acetate followed by methanol. Ellagic acid derivatives were identified on the basis of UV and mass spectra, and the presence of ellagitannins was confirmed by a significant increase in free ellagic acid with HPLC followed by acid hydrolysis. Muscadine grapes contained phenolic acids, flavonols, anthocyanins, ellagic acid, and numerous ellagic acid derivatives. AOX varied in the order ethyl acetate > methanol > C18 nonretained fractions; each correlated to both total phenolics (r = 0.90) and total ellagic acid (r = 0.99) contents. Results of this study revealed previously unidentified ellagic acid derivatives in muscadine grapes. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16028988/Identification_of_ellagic_acid_conjugates_and_other_polyphenolics_in_muscadine_grapes_by_HPLC_ESI_MS_ L2 - https://doi.org/10.1021/jf050468r DB - PRIME DP - Unbound Medicine ER -