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Antioxidant properties of minocycline: neuroprotection in an oxidative stress assay and direct radical-scavenging activity.
J Neurochem. 2005 Aug; 94(3):819-27.JN

Abstract

Minocycline is neuroprotective in animal models of a number of acute CNS injuries and neurodegenerative diseases. While anti-inflammatory and anti-apoptotic effects of minocycline have been characterized, the molecular basis for the neuroprotective effects of minocycline remains unclear. We report here that minocycline and a number of antioxidant compounds protect mixed neuronal cultures in an oxidative stress assay. To evaluate the role of minocycline's direct antioxidant properties in neuroprotection, we determined potencies for minocycline, other tetracycline antibiotics, and reference antioxidant compounds using a panel of in vitro radical scavenging assays. Data from in vitro rat brain homogenate lipid peroxidation and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays show that minocycline, in contrast to tetracycline, is an effective antioxidant with radical scavenging potency similar to vitamin E. Our findings suggest that the direct antioxidant activity of minocycline may contribute to its neuroprotective effects in some cell-based assays and animal models of neuronal injury.

Authors+Show Affiliations

Department of Molecular Neuroscience, Merck Research Laboratories San Diego, California 92121, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16033424

Citation

Kraus, Richard L., et al. "Antioxidant Properties of Minocycline: Neuroprotection in an Oxidative Stress Assay and Direct Radical-scavenging Activity." Journal of Neurochemistry, vol. 94, no. 3, 2005, pp. 819-27.
Kraus RL, Pasieczny R, Lariosa-Willingham K, et al. Antioxidant properties of minocycline: neuroprotection in an oxidative stress assay and direct radical-scavenging activity. J Neurochem. 2005;94(3):819-27.
Kraus, R. L., Pasieczny, R., Lariosa-Willingham, K., Turner, M. S., Jiang, A., & Trauger, J. W. (2005). Antioxidant properties of minocycline: neuroprotection in an oxidative stress assay and direct radical-scavenging activity. Journal of Neurochemistry, 94(3), 819-27.
Kraus RL, et al. Antioxidant Properties of Minocycline: Neuroprotection in an Oxidative Stress Assay and Direct Radical-scavenging Activity. J Neurochem. 2005;94(3):819-27. PubMed PMID: 16033424.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant properties of minocycline: neuroprotection in an oxidative stress assay and direct radical-scavenging activity. AU - Kraus,Richard L, AU - Pasieczny,Rodger, AU - Lariosa-Willingham,Karen, AU - Turner,Mary S, AU - Jiang,Alice, AU - Trauger,John W, PY - 2005/7/22/pubmed PY - 2005/9/20/medline PY - 2005/7/22/entrez SP - 819 EP - 27 JF - Journal of neurochemistry JO - J Neurochem VL - 94 IS - 3 N2 - Minocycline is neuroprotective in animal models of a number of acute CNS injuries and neurodegenerative diseases. While anti-inflammatory and anti-apoptotic effects of minocycline have been characterized, the molecular basis for the neuroprotective effects of minocycline remains unclear. We report here that minocycline and a number of antioxidant compounds protect mixed neuronal cultures in an oxidative stress assay. To evaluate the role of minocycline's direct antioxidant properties in neuroprotection, we determined potencies for minocycline, other tetracycline antibiotics, and reference antioxidant compounds using a panel of in vitro radical scavenging assays. Data from in vitro rat brain homogenate lipid peroxidation and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays show that minocycline, in contrast to tetracycline, is an effective antioxidant with radical scavenging potency similar to vitamin E. Our findings suggest that the direct antioxidant activity of minocycline may contribute to its neuroprotective effects in some cell-based assays and animal models of neuronal injury. SN - 0022-3042 UR - https://www.unboundmedicine.com/medline/citation/16033424/Antioxidant_properties_of_minocycline:_neuroprotection_in_an_oxidative_stress_assay_and_direct_radical_scavenging_activity_ L2 - https://doi.org/10.1111/j.1471-4159.2005.03219.x DB - PRIME DP - Unbound Medicine ER -