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Effect of protective agents, freezing temperature, rehydration media on viability of malolactic bacteria subjected to freeze-drying.
J Appl Microbiol 2005; 99(2):333-8JA

Abstract

AIMS

The effects of protective agents, rehydration media and freezing temperature on the viabilities of Lactobacillus brevis and Oenococcus oeni H-2 when subjected to freeze-drying were investigated.

METHODS AND RESULTS

Several protectants and rehydration media were tested to improve the survival after freeze-drying. The cells were also frozen at -65 and -20 degrees C to check the effect of freezing temperature on the viability.

CONCLUSIONS

The best protectant and rehydration medium to obtain the highest viability after freeze-drying varied with the species of bacteria. Yeast extract (4.0%) and sodium glutamate (2.5%) gave maximum viability of L. brevis and O. oeni (67.8% and 53.6% respectively). The highest survival of L. brevis and O. oeni were obtained when rehydrated with 10% sucrose and MGY medium respectively. When the bacterial cells were frozen quickly (-65 degrees C) than slowly (-20 degrees C), L. brevis and O. oeni both showed increased viability after freeze-drying.

SIGNIFICANCE AND IMPACT OF THE STUDY

The viabilities of L. brevis and O. oeni after freeze-drying were shown to be strain specific and dependent on protective agents, rehydration media and freezing temperature.

Authors+Show Affiliations

Alcoholic Beverage Research Institute, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16033464

Citation

Zhao, G, and G Zhang. "Effect of Protective Agents, Freezing Temperature, Rehydration Media On Viability of Malolactic Bacteria Subjected to Freeze-drying." Journal of Applied Microbiology, vol. 99, no. 2, 2005, pp. 333-8.
Zhao G, Zhang G. Effect of protective agents, freezing temperature, rehydration media on viability of malolactic bacteria subjected to freeze-drying. J Appl Microbiol. 2005;99(2):333-8.
Zhao, G., & Zhang, G. (2005). Effect of protective agents, freezing temperature, rehydration media on viability of malolactic bacteria subjected to freeze-drying. Journal of Applied Microbiology, 99(2), pp. 333-8.
Zhao G, Zhang G. Effect of Protective Agents, Freezing Temperature, Rehydration Media On Viability of Malolactic Bacteria Subjected to Freeze-drying. J Appl Microbiol. 2005;99(2):333-8. PubMed PMID: 16033464.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of protective agents, freezing temperature, rehydration media on viability of malolactic bacteria subjected to freeze-drying. AU - Zhao,G, AU - Zhang,G, PY - 2005/7/22/pubmed PY - 2006/1/21/medline PY - 2005/7/22/entrez SP - 333 EP - 8 JF - Journal of applied microbiology JO - J. Appl. Microbiol. VL - 99 IS - 2 N2 - AIMS: The effects of protective agents, rehydration media and freezing temperature on the viabilities of Lactobacillus brevis and Oenococcus oeni H-2 when subjected to freeze-drying were investigated. METHODS AND RESULTS: Several protectants and rehydration media were tested to improve the survival after freeze-drying. The cells were also frozen at -65 and -20 degrees C to check the effect of freezing temperature on the viability. CONCLUSIONS: The best protectant and rehydration medium to obtain the highest viability after freeze-drying varied with the species of bacteria. Yeast extract (4.0%) and sodium glutamate (2.5%) gave maximum viability of L. brevis and O. oeni (67.8% and 53.6% respectively). The highest survival of L. brevis and O. oeni were obtained when rehydrated with 10% sucrose and MGY medium respectively. When the bacterial cells were frozen quickly (-65 degrees C) than slowly (-20 degrees C), L. brevis and O. oeni both showed increased viability after freeze-drying. SIGNIFICANCE AND IMPACT OF THE STUDY: The viabilities of L. brevis and O. oeni after freeze-drying were shown to be strain specific and dependent on protective agents, rehydration media and freezing temperature. SN - 1364-5072 UR - https://www.unboundmedicine.com/medline/citation/16033464/Effect_of_protective_agents_freezing_temperature_rehydration_media_on_viability_of_malolactic_bacteria_subjected_to_freeze_drying_ L2 - https://doi.org/10.1111/j.1365-2672.2005.02587.x DB - PRIME DP - Unbound Medicine ER -