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Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein.
J Gen Virol. 2005 Aug; 86(Pt 8):2269-2274.JG

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) contains a single spike (S) protein, which binds to its receptor, angiotensin-converting enzyme 2 (ACE2), induces membrane fusion and serves as a neutralizing antigen. A SARS-CoV-S protein-bearing vesicular stomatitis virus (VSV) pseudotype using the VSVDeltaG* system was generated. Partial deletion of the SARS-CoV-S protein cytoplasmic domain allowed efficient incorporation into VSV particles and led to the generation of a pseudotype (VSV-SARS-St19) at high titre. Green fluorescent protein expression was demonstrated as early as 7 h after infection of Vero E6 cells with VSV-SARS-St19. VSV-SARS-St19 was neutralized by anti-SARS-CoV antibody and soluble ACE2, and its infection was blocked by treatment of Vero E6 cells with anti-ACE2 antibody. These results indicated that VSV-SARS-St19 infection is mediated by SARS-CoV-S protein in an ACE2-dependent manner. VSV-SARS-St19 will be useful for analysing the function of SARS-CoV-S protein and for developing rapid methods of detecting neutralizing antibodies specific for SARS-CoV infection.

Authors+Show Affiliations

Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan.Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan.Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan.Laboratory of Respiratory Viral Diseases and SARS, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan.Laboratory of Respiratory Viral Diseases and SARS, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan.Laboratory of Respiratory Viral Diseases and SARS, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan.Laboratory of Influenza Virus, Department of Virology III, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan.Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan.Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16033974

Citation

Fukushi, Shuetsu, et al. "Vesicular Stomatitis Virus Pseudotyped With Severe Acute Respiratory Syndrome Coronavirus Spike Protein." The Journal of General Virology, vol. 86, no. Pt 8, 2005, pp. 2269-2274.
Fukushi S, Mizutani T, Saijo M, et al. Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein. J Gen Virol. 2005;86(Pt 8):2269-2274.
Fukushi, S., Mizutani, T., Saijo, M., Matsuyama, S., Miyajima, N., Taguchi, F., Itamura, S., Kurane, I., & Morikawa, S. (2005). Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein. The Journal of General Virology, 86(Pt 8), 2269-2274. https://doi.org/10.1099/vir.0.80955-0
Fukushi S, et al. Vesicular Stomatitis Virus Pseudotyped With Severe Acute Respiratory Syndrome Coronavirus Spike Protein. J Gen Virol. 2005;86(Pt 8):2269-2274. PubMed PMID: 16033974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein. AU - Fukushi,Shuetsu, AU - Mizutani,Tetsuya, AU - Saijo,Masayuki, AU - Matsuyama,Shutoku, AU - Miyajima,Naoko, AU - Taguchi,Fumihiro, AU - Itamura,Shigeyuki, AU - Kurane,Ichiro, AU - Morikawa,Shigeru, PY - 2005/7/22/pubmed PY - 2005/9/8/medline PY - 2005/7/22/entrez SP - 2269 EP - 2274 JF - The Journal of general virology JO - J Gen Virol VL - 86 IS - Pt 8 N2 - Severe acute respiratory syndrome coronavirus (SARS-CoV) contains a single spike (S) protein, which binds to its receptor, angiotensin-converting enzyme 2 (ACE2), induces membrane fusion and serves as a neutralizing antigen. A SARS-CoV-S protein-bearing vesicular stomatitis virus (VSV) pseudotype using the VSVDeltaG* system was generated. Partial deletion of the SARS-CoV-S protein cytoplasmic domain allowed efficient incorporation into VSV particles and led to the generation of a pseudotype (VSV-SARS-St19) at high titre. Green fluorescent protein expression was demonstrated as early as 7 h after infection of Vero E6 cells with VSV-SARS-St19. VSV-SARS-St19 was neutralized by anti-SARS-CoV antibody and soluble ACE2, and its infection was blocked by treatment of Vero E6 cells with anti-ACE2 antibody. These results indicated that VSV-SARS-St19 infection is mediated by SARS-CoV-S protein in an ACE2-dependent manner. VSV-SARS-St19 will be useful for analysing the function of SARS-CoV-S protein and for developing rapid methods of detecting neutralizing antibodies specific for SARS-CoV infection. SN - 0022-1317 UR - https://www.unboundmedicine.com/medline/citation/16033974/Vesicular_stomatitis_virus_pseudotyped_with_severe_acute_respiratory_syndrome_coronavirus_spike_protein_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/vir.0.80955-0 DB - PRIME DP - Unbound Medicine ER -