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Effect of arachidonic, eicosapentaenoic and docosahexaenoic acids on the oxidative status of C6 glioma cells.
Free Radic Res. 2005 Aug; 39(8):865-74.FR

Abstract

n-3 polyunsaturated fatty acids (PUFAs) have been described to have beneficial effects on brain development and in the prevention and treatment of brain damage. C6 glioma cells were incubated with 100 microM of either C20:4n-6 (ARA), or C20:5n-3 (EPA), or C22:6n-3 (DHA) for different time periods to assess whether these acids altered the cellular oxidative state. The ARA and EPA were promptly metabolised to C22:4n-6 and C22:5n-3, respectively, whereas DHA treatment simply increased the amount of DHA in the cells. Cell viability was not affected by ARA, while a cytotoxic effect was observed 72 h after n-3 PUFAs supplementation. The levels of reactive oxygen species and thiobarbituric acid-reactive substances were significantly higher in DHA-treated cells than in EPA- and ARA-treated groups. This modification in the oxidative cellular status was also highlighted by a significant increase in catalase activity and a decrease in glutathione content in DHA-supplemented cells. Glucose-6-phosphate dehydrogenase activity, an enzyme involved in redox regulation, and O2*- release were significantly increased both in EPA and DHA groups. The effect of DHA was more severe than that of EPA. No significant changes were observed in the ARA group with respect to untreated cells. These data show that EPA and DHA induce alterations in the oxidative status that could affect the glial function.

Authors+Show Affiliations

Food Science, Nutrition and Health Department, Intituto Superiore di Sanità, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16036367

Citation

Leonardi, Fabiana, et al. "Effect of Arachidonic, Eicosapentaenoic and Docosahexaenoic Acids On the Oxidative Status of C6 Glioma Cells." Free Radical Research, vol. 39, no. 8, 2005, pp. 865-74.
Leonardi F, Attorri L, Di Benedetto R, et al. Effect of arachidonic, eicosapentaenoic and docosahexaenoic acids on the oxidative status of C6 glioma cells. Free Radic Res. 2005;39(8):865-74.
Leonardi, F., Attorri, L., Di Benedetto, R., Di Biase, A., Sanchez, M., Nardini, M., & Salvati, S. (2005). Effect of arachidonic, eicosapentaenoic and docosahexaenoic acids on the oxidative status of C6 glioma cells. Free Radical Research, 39(8), 865-74.
Leonardi F, et al. Effect of Arachidonic, Eicosapentaenoic and Docosahexaenoic Acids On the Oxidative Status of C6 Glioma Cells. Free Radic Res. 2005;39(8):865-74. PubMed PMID: 16036367.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of arachidonic, eicosapentaenoic and docosahexaenoic acids on the oxidative status of C6 glioma cells. AU - Leonardi,Fabiana, AU - Attorri,Lucilla, AU - Di Benedetto,Rita, AU - Di Biase,Antonella, AU - Sanchez,Massimo, AU - Nardini,Mirella, AU - Salvati,Serafina, PY - 2005/7/23/pubmed PY - 2005/10/12/medline PY - 2005/7/23/entrez SP - 865 EP - 74 JF - Free radical research JO - Free Radic. Res. VL - 39 IS - 8 N2 - n-3 polyunsaturated fatty acids (PUFAs) have been described to have beneficial effects on brain development and in the prevention and treatment of brain damage. C6 glioma cells were incubated with 100 microM of either C20:4n-6 (ARA), or C20:5n-3 (EPA), or C22:6n-3 (DHA) for different time periods to assess whether these acids altered the cellular oxidative state. The ARA and EPA were promptly metabolised to C22:4n-6 and C22:5n-3, respectively, whereas DHA treatment simply increased the amount of DHA in the cells. Cell viability was not affected by ARA, while a cytotoxic effect was observed 72 h after n-3 PUFAs supplementation. The levels of reactive oxygen species and thiobarbituric acid-reactive substances were significantly higher in DHA-treated cells than in EPA- and ARA-treated groups. This modification in the oxidative cellular status was also highlighted by a significant increase in catalase activity and a decrease in glutathione content in DHA-supplemented cells. Glucose-6-phosphate dehydrogenase activity, an enzyme involved in redox regulation, and O2*- release were significantly increased both in EPA and DHA groups. The effect of DHA was more severe than that of EPA. No significant changes were observed in the ARA group with respect to untreated cells. These data show that EPA and DHA induce alterations in the oxidative status that could affect the glial function. SN - 1071-5762 UR - https://www.unboundmedicine.com/medline/citation/16036367/Effect_of_arachidonic_eicosapentaenoic_and_docosahexaenoic_acids_on_the_oxidative_status_of_C6_glioma_cells_ L2 - http://www.tandfonline.com/doi/full/10.1080/10715760500145069 DB - PRIME DP - Unbound Medicine ER -