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[Altered expression of vascular endothelial growth factor and its receptors in transdifferentiated human proximal tubular epithelial cells induced by transforming growth factor beta1].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005 Jun; 27(3):325-31.ZY

Abstract

OBJECTIVE

To examine the expression of vascular endothelial growth factor (VEGF) and its receptors (VEGFR1, VEGFR2) in transdifferentiated human proximal tubular epithelial (HK-2) cell induced by transforming growth factor beta1 (TGFbeta1).

METHODS

The transdifferentiation of HK-2 cells was detected by evaluation of expression of alpha-SMA by cytoimmunochemistry and RT-PCR. The VEGF mRNA was evaluated with RT-PCR. The secreted VEGF in the culture media was measured with ELISA. The cellular VEGF, VEGFR1, and VEGFR2 were measured with Western blot.

RESULTS

The immunostain of alpha-SMA were positive in HK-2 cell induced by TGFbeta1 at the concentration of 5 and 8 ng/ml for 72 h. The expression of alpha-SMA mRNA was induced by TGFbeta1 in concentration- and time-dependent manners. The expressions of mRNA and protein of VEGF were upregulated by TGFbeta1 at the concentration of 0.1 and 1 ng/ml for 72 h and at the concentration of 8 ng/ml for 12 h and 24 h when compared with the control. But expressions of mRNA and protein of VEGF were downregulated by TGFbeta1 at the concentration of 3, 5, and 8 ng/ml for 72 h and at the concentration of 8 ng/ml for 36, 48, and 72 h, respectively. Meanwhile, Protein levels of VEGFR1 and VEGFR2 were upregulated by TGFbeta1 in concentration- and time- dependent manners.

CONCLUSIONS

Increased expression of VEGFR1 and VEGFR2 and two-phase change in VEGF expression occurred in the process of tubular epithelial transdifferentiation induced by TGFbeta1. Reduced expression of VEGF may contribute to tubular epithelial transdifferentiation in a vicious circle.

Authors+Show Affiliations

Department of Nephrology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

16038269

Citation

Zhou, Qiu-gen, et al. "[Altered Expression of Vascular Endothelial Growth Factor and Its Receptors in Transdifferentiated Human Proximal Tubular Epithelial Cells Induced By Transforming Growth Factor Beta1]." Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, vol. 27, no. 3, 2005, pp. 325-31.
Zhou QG, Zheng FL, Wen YB, et al. [Altered expression of vascular endothelial growth factor and its receptors in transdifferentiated human proximal tubular epithelial cells induced by transforming growth factor beta1]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005;27(3):325-31.
Zhou, Q. G., Zheng, F. L., Wen, Y. B., Tan, X. Y., Duan, L., & Li, Y. (2005). [Altered expression of vascular endothelial growth factor and its receptors in transdifferentiated human proximal tubular epithelial cells induced by transforming growth factor beta1]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, 27(3), 325-31.
Zhou QG, et al. [Altered Expression of Vascular Endothelial Growth Factor and Its Receptors in Transdifferentiated Human Proximal Tubular Epithelial Cells Induced By Transforming Growth Factor Beta1]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005;27(3):325-31. PubMed PMID: 16038269.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Altered expression of vascular endothelial growth factor and its receptors in transdifferentiated human proximal tubular epithelial cells induced by transforming growth factor beta1]. AU - Zhou,Qiu-gen, AU - Zheng,Fa-lei, AU - Wen,Yu-bing, AU - Tan,Xiao-yue, AU - Duan,Lin, AU - Li,Yan, PY - 2005/7/26/pubmed PY - 2006/4/29/medline PY - 2005/7/26/entrez SP - 325 EP - 31 JF - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae JO - Zhongguo Yi Xue Ke Xue Yuan Xue Bao VL - 27 IS - 3 N2 - OBJECTIVE: To examine the expression of vascular endothelial growth factor (VEGF) and its receptors (VEGFR1, VEGFR2) in transdifferentiated human proximal tubular epithelial (HK-2) cell induced by transforming growth factor beta1 (TGFbeta1). METHODS: The transdifferentiation of HK-2 cells was detected by evaluation of expression of alpha-SMA by cytoimmunochemistry and RT-PCR. The VEGF mRNA was evaluated with RT-PCR. The secreted VEGF in the culture media was measured with ELISA. The cellular VEGF, VEGFR1, and VEGFR2 were measured with Western blot. RESULTS: The immunostain of alpha-SMA were positive in HK-2 cell induced by TGFbeta1 at the concentration of 5 and 8 ng/ml for 72 h. The expression of alpha-SMA mRNA was induced by TGFbeta1 in concentration- and time-dependent manners. The expressions of mRNA and protein of VEGF were upregulated by TGFbeta1 at the concentration of 0.1 and 1 ng/ml for 72 h and at the concentration of 8 ng/ml for 12 h and 24 h when compared with the control. But expressions of mRNA and protein of VEGF were downregulated by TGFbeta1 at the concentration of 3, 5, and 8 ng/ml for 72 h and at the concentration of 8 ng/ml for 36, 48, and 72 h, respectively. Meanwhile, Protein levels of VEGFR1 and VEGFR2 were upregulated by TGFbeta1 in concentration- and time- dependent manners. CONCLUSIONS: Increased expression of VEGFR1 and VEGFR2 and two-phase change in VEGF expression occurred in the process of tubular epithelial transdifferentiation induced by TGFbeta1. Reduced expression of VEGF may contribute to tubular epithelial transdifferentiation in a vicious circle. SN - 1000-503X UR - https://www.unboundmedicine.com/medline/citation/16038269/[Altered_expression_of_vascular_endothelial_growth_factor_and_its_receptors_in_transdifferentiated_human_proximal_tubular_epithelial_cells_induced_by_transforming_growth_factor_beta1]_ L2 - https://antibodies.cancer.gov/detail/CPTC-TGFB1-1 DB - PRIME DP - Unbound Medicine ER -