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Functional role of prostacyclin receptor in rat dorsal root ganglion neurons.
Neurosci Lett. 2005 Nov 18; 388(3):132-7.NL

Abstract

Recent studies on prostanoids showed that some of prostanoid receptors are expressed in rat dorsal root ganglion (DRG) neurons. These facts suggest that prostanoid receptors might be involved in the excitation mechanism of DRG neurons. In the present study, PCR experiments revealed that one of prostanoid receptor, prostacyclin receptor (IP receptor) was expressed in L6 and S1 rat DRG neurons and that the expression of IP receptor was not changed in DRG neurons obtained from the cyclophosphamide (CYP)-induced cystitis rat. We examined the functional role of IP receptor agonist and other prostanoids by measuring cyclic AMP (cAMP) accumulation and substance P (SP) release in primary cultured DRG neurons. The pretreatment of DRG neurons with prostanoid agonists such as iloprost (IP), butaprost (EP(2)), misoprostol (EP(2-4)), PGE(2) (EP(1-4)) or PGD(2) (DP and CRTH2) sensitized the DRG neurons and hence potentiated the lys-bradykinin-induced SP release. The increase of SP release by lys-BK plus prostanoid agonists was proportion to cAMP accumulation. Iloprost was the most potent agonist to induce cAMP accumulation and SP release among prostanoid agonists evaluated in this study and its effect is mediated by IP receptor. Moreover, capsaicin-, ATP- and KCl-induced SP release was also enhanced by iloprost although iloprost did not change intracellular Ca(2+) and membrane depolarization induced by these chemical stimuli. These results strongly indicate that IP receptor play an important role in the sensitization of rat sensory neuron.

Authors+Show Affiliations

TRA Urology, Research Center Kyoto, Bayer Yakuhin, Ltd., 6-5-1-3 Kunimidai, Kizu-cho, Soraku-gun, Kyoto 619-0216, Japan. nakaek@bikaken.or.joNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16039053

Citation

Nakae, Koichi, et al. "Functional Role of Prostacyclin Receptor in Rat Dorsal Root Ganglion Neurons." Neuroscience Letters, vol. 388, no. 3, 2005, pp. 132-7.
Nakae K, Hayashi F, Hayashi M, et al. Functional role of prostacyclin receptor in rat dorsal root ganglion neurons. Neurosci Lett. 2005;388(3):132-7.
Nakae, K., Hayashi, F., Hayashi, M., Yamamoto, N., Iino, T., Yoshikawa, S., & Gupta, J. (2005). Functional role of prostacyclin receptor in rat dorsal root ganglion neurons. Neuroscience Letters, 388(3), 132-7.
Nakae K, et al. Functional Role of Prostacyclin Receptor in Rat Dorsal Root Ganglion Neurons. Neurosci Lett. 2005 Nov 18;388(3):132-7. PubMed PMID: 16039053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional role of prostacyclin receptor in rat dorsal root ganglion neurons. AU - Nakae,Koichi, AU - Hayashi,Fumihiko, AU - Hayashi,Mayumi, AU - Yamamoto,Noriyuki, AU - Iino,Takashi, AU - Yoshikawa,Satoru, AU - Gupta,Jang, PY - 2005/05/24/received PY - 2005/06/24/revised PY - 2005/06/25/accepted PY - 2005/7/26/pubmed PY - 2006/1/24/medline PY - 2005/7/26/entrez SP - 132 EP - 7 JF - Neuroscience letters JO - Neurosci. Lett. VL - 388 IS - 3 N2 - Recent studies on prostanoids showed that some of prostanoid receptors are expressed in rat dorsal root ganglion (DRG) neurons. These facts suggest that prostanoid receptors might be involved in the excitation mechanism of DRG neurons. In the present study, PCR experiments revealed that one of prostanoid receptor, prostacyclin receptor (IP receptor) was expressed in L6 and S1 rat DRG neurons and that the expression of IP receptor was not changed in DRG neurons obtained from the cyclophosphamide (CYP)-induced cystitis rat. We examined the functional role of IP receptor agonist and other prostanoids by measuring cyclic AMP (cAMP) accumulation and substance P (SP) release in primary cultured DRG neurons. The pretreatment of DRG neurons with prostanoid agonists such as iloprost (IP), butaprost (EP(2)), misoprostol (EP(2-4)), PGE(2) (EP(1-4)) or PGD(2) (DP and CRTH2) sensitized the DRG neurons and hence potentiated the lys-bradykinin-induced SP release. The increase of SP release by lys-BK plus prostanoid agonists was proportion to cAMP accumulation. Iloprost was the most potent agonist to induce cAMP accumulation and SP release among prostanoid agonists evaluated in this study and its effect is mediated by IP receptor. Moreover, capsaicin-, ATP- and KCl-induced SP release was also enhanced by iloprost although iloprost did not change intracellular Ca(2+) and membrane depolarization induced by these chemical stimuli. These results strongly indicate that IP receptor play an important role in the sensitization of rat sensory neuron. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/16039053/Functional_role_of_prostacyclin_receptor_in_rat_dorsal_root_ganglion_neurons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(05)00762-7 DB - PRIME DP - Unbound Medicine ER -