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Mast cell mediators in citric acid-induced airway constriction of guinea pigs.
Toxicol Appl Pharmacol. 2005 Aug 15; 206(3):343-50.TA

Abstract

We demonstrated previously that mast cells play an important role in citric acid (CA)-induced airway constriction. In this study, we further investigated the underlying mediator(s) for this type of airway constriction. At first, to examine effects caused by blocking agents, 67 young Hartley guinea pigs were divided into 7 groups: saline + CA; methysergide (serotonin receptor antagonist) + CA; MK-886 (leukotriene synthesis inhibitor) + CA; mepyramine (histamine H1 receptor antagonist) + CA; indomethacin (cyclooxygenase inhibitor) + CA; cromolyn sodium (mast cell stabilizer) + CA; and compound 48/80 (mast cell degranulating agent) + CA. Then, we tested whether leukotriene C4 (LTC4) or histamine enhances CA-induced airway constriction in compound 48/80-pretreated guinea pigs. We measured dynamic respiratory compliance (Crs) and forced expiratory volume in 0.1 s (FEV0.1) during either baseline or recovery period. In addition, we detected histamine level, an index of pulmonary mast cell degranulation, in bronchoalveolar lavage (BAL) samples. Citric acid aerosol inhalation caused decreases in Crs and FEV0.1, indicating airway constriction in the control group. This airway constriction was significantly attenuated by MK-886, mepyramine, cromolyn sodium, and compound 48/80, but not by either methysergide or indomethacin. Both LTC4 and histamine infusion significantly increased the magnitude of CA-induced airway constriction in compound 48/80-pretreated guinea pigs. Citric acid inhalation caused significant increase in histamine level in the BAL sample, which was significantly suppressed by compound 48/80. These results suggest that leukotrienes and histamine originating from mast cells play an important role in CA inhalation-induced noncholinergic airway constriction.

Authors+Show Affiliations

Department of Physiology, College of Medicine, National Taiwan University, No. 1, Sec. 1, Jen-Ai Road, Taipei 100, Taiwan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16039945

Citation

Lin, Chien-He, and Yih-Loong Lai. "Mast Cell Mediators in Citric Acid-induced Airway Constriction of Guinea Pigs." Toxicology and Applied Pharmacology, vol. 206, no. 3, 2005, pp. 343-50.
Lin CH, Lai YL. Mast cell mediators in citric acid-induced airway constriction of guinea pigs. Toxicol Appl Pharmacol. 2005;206(3):343-50.
Lin, C. H., & Lai, Y. L. (2005). Mast cell mediators in citric acid-induced airway constriction of guinea pigs. Toxicology and Applied Pharmacology, 206(3), 343-50.
Lin CH, Lai YL. Mast Cell Mediators in Citric Acid-induced Airway Constriction of Guinea Pigs. Toxicol Appl Pharmacol. 2005 Aug 15;206(3):343-50. PubMed PMID: 16039945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mast cell mediators in citric acid-induced airway constriction of guinea pigs. AU - Lin,Chien-He, AU - Lai,Yih-Loong, PY - 2004/08/22/received PY - 2004/11/09/revised PY - 2004/11/09/accepted PY - 2005/7/26/pubmed PY - 2005/9/16/medline PY - 2005/7/26/entrez SP - 343 EP - 50 JF - Toxicology and applied pharmacology JO - Toxicol Appl Pharmacol VL - 206 IS - 3 N2 - We demonstrated previously that mast cells play an important role in citric acid (CA)-induced airway constriction. In this study, we further investigated the underlying mediator(s) for this type of airway constriction. At first, to examine effects caused by blocking agents, 67 young Hartley guinea pigs were divided into 7 groups: saline + CA; methysergide (serotonin receptor antagonist) + CA; MK-886 (leukotriene synthesis inhibitor) + CA; mepyramine (histamine H1 receptor antagonist) + CA; indomethacin (cyclooxygenase inhibitor) + CA; cromolyn sodium (mast cell stabilizer) + CA; and compound 48/80 (mast cell degranulating agent) + CA. Then, we tested whether leukotriene C4 (LTC4) or histamine enhances CA-induced airway constriction in compound 48/80-pretreated guinea pigs. We measured dynamic respiratory compliance (Crs) and forced expiratory volume in 0.1 s (FEV0.1) during either baseline or recovery period. In addition, we detected histamine level, an index of pulmonary mast cell degranulation, in bronchoalveolar lavage (BAL) samples. Citric acid aerosol inhalation caused decreases in Crs and FEV0.1, indicating airway constriction in the control group. This airway constriction was significantly attenuated by MK-886, mepyramine, cromolyn sodium, and compound 48/80, but not by either methysergide or indomethacin. Both LTC4 and histamine infusion significantly increased the magnitude of CA-induced airway constriction in compound 48/80-pretreated guinea pigs. Citric acid inhalation caused significant increase in histamine level in the BAL sample, which was significantly suppressed by compound 48/80. These results suggest that leukotrienes and histamine originating from mast cells play an important role in CA inhalation-induced noncholinergic airway constriction. SN - 0041-008X UR - https://www.unboundmedicine.com/medline/citation/16039945/Mast_cell_mediators_in_citric_acid_induced_airway_constriction_of_guinea_pigs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-008X(04)00533-2 DB - PRIME DP - Unbound Medicine ER -