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Direct electrochemistry and electrocatalytic activity of catalase incorporated onto multiwall carbon nanotubes-modified glassy carbon electrode.
Anal Biochem. 2005 Sep 01; 344(1):16-24.AB

Abstract

The direct voltammetry and electrocatalytic properties of catalase, which was adsorbed on the surface of multiwall carbon nanotubes (MWCNTs), was investigated. A pair of well-defined and nearly reversible cyclic voltammetry peaks for Fe(III)/Fe(II) redox couple of catalase adsorbed on the surface of MWCNTs at approximately -0.05 V versus reference electrode in pH 6.5 buffer solution, indicating the direct electron transfer between catalase and electrode. The surface coverage of catalase immobilized on MWCNTs glassy carbon electrode was approximately 2.4x10(-10) molcm-2. The transfer coefficient (alpha) was calculated to be 0.4, and the heterogeneous electron transfer rate constant was 80 s-1 in pH 7, indicating great facilitation of the electron transfer between catalase and MWCNTs adsorbed on the electrode surface. The formal potential of catalase Fe(III)/Fe(II) couple in MWCNTs film had a linear relationship with pH values between 2 and 11 with a slope of 58 mV/pH, showing that the electron transfer is accompanied by single proton transportation. Catalase adsorbed on MWCNTs exhibits a remarkable electrocatalytic activity toward the reduction of oxygen and hydrogen peroxide. The value for calculated Michaelis-Menten constant (1.70 mM) was high, indicating the potential applicability of the films as a new type of reagentless biosensor based on the direct electrochemistry of the catalase enzyme.

Authors+Show Affiliations

Department of Chemistry, Kurdistan University, P.O. Box 416, Sanandaj, Iran. absalimi@yahoo.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16039977

Citation

Salimi, Abdollah, et al. "Direct Electrochemistry and Electrocatalytic Activity of Catalase Incorporated Onto Multiwall Carbon Nanotubes-modified Glassy Carbon Electrode." Analytical Biochemistry, vol. 344, no. 1, 2005, pp. 16-24.
Salimi A, Noorbakhsh A, Ghadermarz M. Direct electrochemistry and electrocatalytic activity of catalase incorporated onto multiwall carbon nanotubes-modified glassy carbon electrode. Anal Biochem. 2005;344(1):16-24.
Salimi, A., Noorbakhsh, A., & Ghadermarz, M. (2005). Direct electrochemistry and electrocatalytic activity of catalase incorporated onto multiwall carbon nanotubes-modified glassy carbon electrode. Analytical Biochemistry, 344(1), 16-24.
Salimi A, Noorbakhsh A, Ghadermarz M. Direct Electrochemistry and Electrocatalytic Activity of Catalase Incorporated Onto Multiwall Carbon Nanotubes-modified Glassy Carbon Electrode. Anal Biochem. 2005 Sep 1;344(1):16-24. PubMed PMID: 16039977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct electrochemistry and electrocatalytic activity of catalase incorporated onto multiwall carbon nanotubes-modified glassy carbon electrode. AU - Salimi,Abdollah, AU - Noorbakhsh,Abdollah, AU - Ghadermarz,Mahmoud, PY - 2005/02/16/received PY - 2005/05/21/revised PY - 2005/05/25/accepted PY - 2005/7/26/pubmed PY - 2005/12/15/medline PY - 2005/7/26/entrez SP - 16 EP - 24 JF - Analytical biochemistry JO - Anal Biochem VL - 344 IS - 1 N2 - The direct voltammetry and electrocatalytic properties of catalase, which was adsorbed on the surface of multiwall carbon nanotubes (MWCNTs), was investigated. A pair of well-defined and nearly reversible cyclic voltammetry peaks for Fe(III)/Fe(II) redox couple of catalase adsorbed on the surface of MWCNTs at approximately -0.05 V versus reference electrode in pH 6.5 buffer solution, indicating the direct electron transfer between catalase and electrode. The surface coverage of catalase immobilized on MWCNTs glassy carbon electrode was approximately 2.4x10(-10) molcm-2. The transfer coefficient (alpha) was calculated to be 0.4, and the heterogeneous electron transfer rate constant was 80 s-1 in pH 7, indicating great facilitation of the electron transfer between catalase and MWCNTs adsorbed on the electrode surface. The formal potential of catalase Fe(III)/Fe(II) couple in MWCNTs film had a linear relationship with pH values between 2 and 11 with a slope of 58 mV/pH, showing that the electron transfer is accompanied by single proton transportation. Catalase adsorbed on MWCNTs exhibits a remarkable electrocatalytic activity toward the reduction of oxygen and hydrogen peroxide. The value for calculated Michaelis-Menten constant (1.70 mM) was high, indicating the potential applicability of the films as a new type of reagentless biosensor based on the direct electrochemistry of the catalase enzyme. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/16039977/Direct_electrochemistry_and_electrocatalytic_activity_of_catalase_incorporated_onto_multiwall_carbon_nanotubes_modified_glassy_carbon_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(05)00410-0 DB - PRIME DP - Unbound Medicine ER -