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Free radical scavenging, enzyme inhibitory constituents from antidiabetic Ayurvedic medicinal plant Hydnocarpus wightiana Blume.
Phytother Res. 2005 Apr; 19(4):277-81.PR

Abstract

Hydnocarpus wightiana is advocated in traditional Indian medicine to possess strong antidiabetic activity. In the course of identifying bioactive fractions from Indian medicinal plants we observed that acetone extract of the seed hulls of H. wightiana possess strong free radicals (DPPH and ABTS) scavenging, alpha-glucosidase and moderate N-acetyl-beta-D-glucosaminidase inhibitory activities. Further fractionation of the extract led to the isolation of hydnocarpin, luteolin and isohydnocarpin in substantial yields. All the compounds showed strong ABTS scavenging property. However, only luteolin could display strong DPPH scavenging activity. Furthermore, all the three compounds also showed varying degrees of alpha-glucosidase and N-acetyl-beta-D-glucosaminidase inhibitory activity, luteolin being the superior. The kinetics of alpha-glucosidase inhibition by these compounds showed that acetone extract inhibits the enzyme in competitive manner however, luteolin and isohydnocarpin showed mixed-type inhibition. This is the first report assigning hydnocarpin and isohydnocarpin free radical scavenging, alpha-glucosidase and N-acetyl-beta-D-glucosaminidase inhibitory properties and luteolin as N-acetyl-beta-D-glucosaminidase inhibitor. This study suggests that presence of amphiphilic antioxidant molecules along with enzyme inhibitory activities in the acetone extract of H. wightiana seed hulls may be responsible for the antidiabetic properties as advocated in traditional medicine.

Authors+Show Affiliations

Natural Products Laboratory, Division of Organic Chemistry-I, Indian Institute of Chemical Technology, Hyderabad, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16041766

Citation

Reddy, S Venkat, et al. "Free Radical Scavenging, Enzyme Inhibitory Constituents From Antidiabetic Ayurvedic Medicinal Plant Hydnocarpus Wightiana Blume." Phytotherapy Research : PTR, vol. 19, no. 4, 2005, pp. 277-81.
Reddy SV, Tiwari AK, Kumar US, et al. Free radical scavenging, enzyme inhibitory constituents from antidiabetic Ayurvedic medicinal plant Hydnocarpus wightiana Blume. Phytother Res. 2005;19(4):277-81.
Reddy, S. V., Tiwari, A. K., Kumar, U. S., Rao, R. J., & Rao, J. M. (2005). Free radical scavenging, enzyme inhibitory constituents from antidiabetic Ayurvedic medicinal plant Hydnocarpus wightiana Blume. Phytotherapy Research : PTR, 19(4), 277-81.
Reddy SV, et al. Free Radical Scavenging, Enzyme Inhibitory Constituents From Antidiabetic Ayurvedic Medicinal Plant Hydnocarpus Wightiana Blume. Phytother Res. 2005;19(4):277-81. PubMed PMID: 16041766.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Free radical scavenging, enzyme inhibitory constituents from antidiabetic Ayurvedic medicinal plant Hydnocarpus wightiana Blume. AU - Reddy,S Venkat, AU - Tiwari,Ashok K, AU - Kumar,U Sampath, AU - Rao,R Jagadeeshwar, AU - Rao,J Madhusudan, PY - 2005/7/26/pubmed PY - 2005/9/24/medline PY - 2005/7/26/entrez SP - 277 EP - 81 JF - Phytotherapy research : PTR JO - Phytother Res VL - 19 IS - 4 N2 - Hydnocarpus wightiana is advocated in traditional Indian medicine to possess strong antidiabetic activity. In the course of identifying bioactive fractions from Indian medicinal plants we observed that acetone extract of the seed hulls of H. wightiana possess strong free radicals (DPPH and ABTS) scavenging, alpha-glucosidase and moderate N-acetyl-beta-D-glucosaminidase inhibitory activities. Further fractionation of the extract led to the isolation of hydnocarpin, luteolin and isohydnocarpin in substantial yields. All the compounds showed strong ABTS scavenging property. However, only luteolin could display strong DPPH scavenging activity. Furthermore, all the three compounds also showed varying degrees of alpha-glucosidase and N-acetyl-beta-D-glucosaminidase inhibitory activity, luteolin being the superior. The kinetics of alpha-glucosidase inhibition by these compounds showed that acetone extract inhibits the enzyme in competitive manner however, luteolin and isohydnocarpin showed mixed-type inhibition. This is the first report assigning hydnocarpin and isohydnocarpin free radical scavenging, alpha-glucosidase and N-acetyl-beta-D-glucosaminidase inhibitory properties and luteolin as N-acetyl-beta-D-glucosaminidase inhibitor. This study suggests that presence of amphiphilic antioxidant molecules along with enzyme inhibitory activities in the acetone extract of H. wightiana seed hulls may be responsible for the antidiabetic properties as advocated in traditional medicine. SN - 0951-418X UR - https://www.unboundmedicine.com/medline/citation/16041766/Free_radical_scavenging_enzyme_inhibitory_constituents_from_antidiabetic_Ayurvedic_medicinal_plant_Hydnocarpus_wightiana_Blume_ L2 - https://doi.org/10.1002/ptr.1491 DB - PRIME DP - Unbound Medicine ER -