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[Antigen analysis of Angiostrongylus cantonensis in different developmental stages].

Abstract

OBJECTIVE

To analyze the difference among antigens of Angiostrongylus cantonensis in different developmental stages and identify dominant diagnostic antigen for angiostrongyliasis.

METHODS

Antigens of A. cantonensis in different developmental stages were analyzed by SDS-PAGE and immunoblot.

RESULTS

The protein bands of all developmental stages were similar on SDS-PAGE. The Mr 40000, 50000, 66000 and 80000 antigens reacted not only with the sera of rats infected by A. cantonensis but also with the sera of normal rats. The Mr 104000 antigen could be discerned by sera of rats infected with A. cantonensis for 2 weeks. The Mr 32000 antigen could be recognized by sera of rats 2 weeks after infection, and the reaction became stronger with the infection continued.

CONCLUSION

The Mr 40000, 50000, 66000 and 80000 antigens might result in the unspecific reaction in the immunodiagnosis of angiostrongyliasis using the crude antigen of A. cantonensis. The Mr 104000 of larva, Mr 33000 of adult females and Mr 32000 of the worms might be used as candidate antigens in early diagnosis and epidemiological survey of angiostrongyliasis.

Authors+Show Affiliations

Department of Parasitology, Southern Medical University, Guangzhou 510515, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16042205

Citation

Li, Hua, et al. "[Antigen Analysis of Angiostrongylus Cantonensis in Different Developmental Stages]." Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases, vol. 23, no. 1, 2005, pp. 36-9.
Li H, Chen XG, Shen HX, et al. [Antigen analysis of Angiostrongylus cantonensis in different developmental stages]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2005;23(1):36-9.
Li, H., Chen, X. G., Shen, H. X., Peng, H. J., & Zhao, X. C. (2005). [Antigen analysis of Angiostrongylus cantonensis in different developmental stages]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases, 23(1), 36-9.
Li H, et al. [Antigen Analysis of Angiostrongylus Cantonensis in Different Developmental Stages]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2005 Feb 28;23(1):36-9. PubMed PMID: 16042205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Antigen analysis of Angiostrongylus cantonensis in different developmental stages]. AU - Li,Hua, AU - Chen,Xiao-guang, AU - Shen,Hao-xian, AU - Peng,Hong-juan, AU - Zhao,Xing-cun, PY - 2005/7/27/pubmed PY - 2006/11/11/medline PY - 2005/7/27/entrez SP - 36 EP - 9 JF - Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases JO - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi VL - 23 IS - 1 N2 - OBJECTIVE: To analyze the difference among antigens of Angiostrongylus cantonensis in different developmental stages and identify dominant diagnostic antigen for angiostrongyliasis. METHODS: Antigens of A. cantonensis in different developmental stages were analyzed by SDS-PAGE and immunoblot. RESULTS: The protein bands of all developmental stages were similar on SDS-PAGE. The Mr 40000, 50000, 66000 and 80000 antigens reacted not only with the sera of rats infected by A. cantonensis but also with the sera of normal rats. The Mr 104000 antigen could be discerned by sera of rats infected with A. cantonensis for 2 weeks. The Mr 32000 antigen could be recognized by sera of rats 2 weeks after infection, and the reaction became stronger with the infection continued. CONCLUSION: The Mr 40000, 50000, 66000 and 80000 antigens might result in the unspecific reaction in the immunodiagnosis of angiostrongyliasis using the crude antigen of A. cantonensis. The Mr 104000 of larva, Mr 33000 of adult females and Mr 32000 of the worms might be used as candidate antigens in early diagnosis and epidemiological survey of angiostrongyliasis. SN - 1000-7423 UR - https://www.unboundmedicine.com/medline/citation/16042205/[Antigen_analysis_of_Angiostrongylus_cantonensis_in_different_developmental_stages]_ DB - PRIME DP - Unbound Medicine ER -