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Exhaled 8-isoprostane in childhood asthma.
Respir Res. 2005 Jul 21; 6:79.RR

Abstract

BACKGROUND

Exhaled breath condensate (EBC) is a non-invasive method to assess airway inflammation and oxidative stress and may be useful in the assessment of childhood asthma.

METHODS

Exhaled 8-isoprostane, a stable marker of oxidative stress, was measured in EBC, in children (5-17 years) with asthma (13 steroid-naïve and 12 inhaled steroid-treated) and 11 healthy control.

RESULTS

Mean exhaled 8-isoprostane concentration was significantly elevated in steroid-naïve asthmatic children compared to healthy children 9.3 (SEM 1.7) vs. 3.8 (0.6) pg/ml, p < 0.01. Children on inhaled steroids also had significantly higher 8-isoprostane levels than those of normal subjects 6.7 (0.7) vs. 3.8 (0.6) pg/ml, p < 0.01. Steroid-naïve asthmatics had higher exhaled nitric oxide (eNO) than those of controls 28.5 (4.7) vs. 12.6 (1.5) ppb, p < 0.01. eNO in steroid-treated asthmatics was similar to control subjects 27.5(8.8) vs. 12.6(1.5) ppb. Exhaled 8-isoprostane did not correlate with duration of asthma, dose of inhaled steroids or eNO.

CONCLUSION

We conclude that 8-isoprostane is elevated in asthmatic children, indicating increased oxidative stress, and that this does not appear to be normalized by inhaled steroid therapy. This suggests that 8-isoprostane is a useful non-invasive measurement of oxidative stress in children and that antioxidant therapy may be useful in the future.

Authors+Show Affiliations

National Heart and Lung Institute, Imperial College London, London, UK. a.bush@ic.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Controlled Clinical Trial
Journal Article

Language

eng

PubMed ID

16042771

Citation

Shahid, Sukhbir K., et al. "Exhaled 8-isoprostane in Childhood Asthma." Respiratory Research, vol. 6, 2005, p. 79.
Shahid SK, Kharitonov SA, Wilson NM, et al. Exhaled 8-isoprostane in childhood asthma. Respir Res. 2005;6:79.
Shahid, S. K., Kharitonov, S. A., Wilson, N. M., Bush, A., & Barnes, P. J. (2005). Exhaled 8-isoprostane in childhood asthma. Respiratory Research, 6, 79.
Shahid SK, et al. Exhaled 8-isoprostane in Childhood Asthma. Respir Res. 2005 Jul 21;6:79. PubMed PMID: 16042771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exhaled 8-isoprostane in childhood asthma. AU - Shahid,Sukhbir K, AU - Kharitonov,Sergei A, AU - Wilson,Nicola M, AU - Bush,Andrew, AU - Barnes,Peter J, Y1 - 2005/07/21/ PY - 2005/02/21/received PY - 2005/07/21/accepted PY - 2005/7/27/pubmed PY - 2006/3/30/medline PY - 2005/7/27/entrez SP - 79 EP - 79 JF - Respiratory research JO - Respir Res VL - 6 N2 - BACKGROUND: Exhaled breath condensate (EBC) is a non-invasive method to assess airway inflammation and oxidative stress and may be useful in the assessment of childhood asthma. METHODS: Exhaled 8-isoprostane, a stable marker of oxidative stress, was measured in EBC, in children (5-17 years) with asthma (13 steroid-naïve and 12 inhaled steroid-treated) and 11 healthy control. RESULTS: Mean exhaled 8-isoprostane concentration was significantly elevated in steroid-naïve asthmatic children compared to healthy children 9.3 (SEM 1.7) vs. 3.8 (0.6) pg/ml, p < 0.01. Children on inhaled steroids also had significantly higher 8-isoprostane levels than those of normal subjects 6.7 (0.7) vs. 3.8 (0.6) pg/ml, p < 0.01. Steroid-naïve asthmatics had higher exhaled nitric oxide (eNO) than those of controls 28.5 (4.7) vs. 12.6 (1.5) ppb, p < 0.01. eNO in steroid-treated asthmatics was similar to control subjects 27.5(8.8) vs. 12.6(1.5) ppb. Exhaled 8-isoprostane did not correlate with duration of asthma, dose of inhaled steroids or eNO. CONCLUSION: We conclude that 8-isoprostane is elevated in asthmatic children, indicating increased oxidative stress, and that this does not appear to be normalized by inhaled steroid therapy. This suggests that 8-isoprostane is a useful non-invasive measurement of oxidative stress in children and that antioxidant therapy may be useful in the future. SN - 1465-993X UR - https://www.unboundmedicine.com/medline/citation/16042771/Exhaled_8_isoprostane_in_childhood_asthma_ L2 - https://respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-6-79 DB - PRIME DP - Unbound Medicine ER -