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Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice.
Vaccine. 2005 Oct 25; 23(44):5196-203.V

Abstract

In this study, the haemolytic activities of Astragalus membranaceus saponins (AMS) and its adjuvant potentials on the cellular and humoral immune responses of ICR mice against OVA were evaluated. We determined the haemolytic activity of AMS using 0.5% rabbit red blood cell. AMS showed a slight haemolytic effect, with its haemolytic percent being 0.66% at the concentration of 500 microg/ml. Furthermore, the adjuvant potentials of AMS at three dose levels on the cellular and humoral immune responses of ICR mice against ovalbumin (OVA) were investigated. ICR mice were immunized subcutaneously with OVA 100 microg alone or with OVA 100 microg dissolved in saline containing Alum (200 microg), QuilA (10 and 20 microg) or AMS (50, 100 or 200 microg) on Day 1 and 15. Two weeks later (Day 28), concanavalin A (Con A)-, lipopolysaccharide (LPS)- and OVA-stimulated splenocyte proliferation and OVA-specific antibodies in serum were measured. AMS significantly enhanced the Con A-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice especially at a dose of 100 microg (P<0.05 or P<0.001). OVA-specific IgG, IgG1 and IgG2b antibody titers in serum were also significantly enhanced by AMS compared with OVA control group (P<0.01 or P<0.001). Moreover, no significant differences (P>0.05) were observed between enhancing effect of AMS and QuilA on the OVA-specific IgG, IgG1 and IgG2b antibody responses to OVA in mice. In conclusion, the results suggest that AMS could be safely used as adjuvant with low or non-haemolytic effect.

Authors+Show Affiliations

College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16043270

Citation

Yang, Zhi-Gang, et al. "Haemolytic Activities and Adjuvant Effect of Astragalus Membranaceus Saponins (AMS) On the Immune Responses to Ovalbumin in Mice." Vaccine, vol. 23, no. 44, 2005, pp. 5196-203.
Yang ZG, Sun HX, Fang WH. Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice. Vaccine. 2005;23(44):5196-203.
Yang, Z. G., Sun, H. X., & Fang, W. H. (2005). Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice. Vaccine, 23(44), 5196-203.
Yang ZG, Sun HX, Fang WH. Haemolytic Activities and Adjuvant Effect of Astragalus Membranaceus Saponins (AMS) On the Immune Responses to Ovalbumin in Mice. Vaccine. 2005 Oct 25;23(44):5196-203. PubMed PMID: 16043270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice. AU - Yang,Zhi-Gang, AU - Sun,Hong-Xiang, AU - Fang,Wei-Huan, PY - 2004/10/11/received PY - 2005/06/01/accepted PY - 2005/7/27/pubmed PY - 2006/1/5/medline PY - 2005/7/27/entrez SP - 5196 EP - 203 JF - Vaccine JO - Vaccine VL - 23 IS - 44 N2 - In this study, the haemolytic activities of Astragalus membranaceus saponins (AMS) and its adjuvant potentials on the cellular and humoral immune responses of ICR mice against OVA were evaluated. We determined the haemolytic activity of AMS using 0.5% rabbit red blood cell. AMS showed a slight haemolytic effect, with its haemolytic percent being 0.66% at the concentration of 500 microg/ml. Furthermore, the adjuvant potentials of AMS at three dose levels on the cellular and humoral immune responses of ICR mice against ovalbumin (OVA) were investigated. ICR mice were immunized subcutaneously with OVA 100 microg alone or with OVA 100 microg dissolved in saline containing Alum (200 microg), QuilA (10 and 20 microg) or AMS (50, 100 or 200 microg) on Day 1 and 15. Two weeks later (Day 28), concanavalin A (Con A)-, lipopolysaccharide (LPS)- and OVA-stimulated splenocyte proliferation and OVA-specific antibodies in serum were measured. AMS significantly enhanced the Con A-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice especially at a dose of 100 microg (P<0.05 or P<0.001). OVA-specific IgG, IgG1 and IgG2b antibody titers in serum were also significantly enhanced by AMS compared with OVA control group (P<0.01 or P<0.001). Moreover, no significant differences (P>0.05) were observed between enhancing effect of AMS and QuilA on the OVA-specific IgG, IgG1 and IgG2b antibody responses to OVA in mice. In conclusion, the results suggest that AMS could be safely used as adjuvant with low or non-haemolytic effect. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/16043270/Haemolytic_activities_and_adjuvant_effect_of_Astragalus_membranaceus_saponins__AMS__on_the_immune_responses_to_ovalbumin_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(05)00604-3 DB - PRIME DP - Unbound Medicine ER -