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Morphological evidence for GABA/glycine-cocontaining terminals in synaptic contact with neurokinin-1 receptor-expressing neurons in the sacral dorsal commissural nucleus of the rat.
Neurosci Lett. 2005 Nov 18; 388(3):144-8.NL

Abstract

Previous studies have shown that neurons in the sacral dorsal commissural nucleus (SDCN) express neurokinin-1 receptor (NK1R) and can be modulated by the co-release of GABA and glycine (Gly) from single presynaptic terminal. These results raise the possibility that GABA/Gly-cocontaining terminals might make synaptic contacts with NK1R-expressing neurons in the SDCN. In order to provide morphological evidence for this hypothesis, the triple-immunohistochemical studies were performed in the SDCN. Triple-immunofluorescence histochemical study showed that some axon terminals in close association with NK1R-immunopositive (NK1R-ip) neurons in the SDCN were immunopositive for both glutamic acid decarboxylase (GAD) and glycine transporter 2 (GlyT2). In electron microscopic dual- and triple-immunohistochemistry for GAD/GlyT2, GAD/NK1R, GlyT2/NK1R, or GAD/GlyT2/NK1R also revealed dually labeled (GAD/GlyT2-ip) synaptic terminals upon SDCN neurons, as well as GAD- and/or GlyT2-ip axon terminals in synaptic contact with NK1R-ip SDCN neurons. These results suggested that some synaptic terminals upon NK1R-expressing SDCN neurons co-released both GABA and Gly.

Authors+Show Affiliations

Department of Anatomy and K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16043285

Citation

Feng, Yu-Peng, et al. "Morphological Evidence for GABA/glycine-cocontaining Terminals in Synaptic Contact With Neurokinin-1 Receptor-expressing Neurons in the Sacral Dorsal Commissural Nucleus of the Rat." Neuroscience Letters, vol. 388, no. 3, 2005, pp. 144-8.
Feng YP, Li YQ, Wang W, et al. Morphological evidence for GABA/glycine-cocontaining terminals in synaptic contact with neurokinin-1 receptor-expressing neurons in the sacral dorsal commissural nucleus of the rat. Neurosci Lett. 2005;388(3):144-8.
Feng, Y. P., Li, Y. Q., Wang, W., Wu, S. X., Chen, T., Shigemoto, R., & Mizuno, N. (2005). Morphological evidence for GABA/glycine-cocontaining terminals in synaptic contact with neurokinin-1 receptor-expressing neurons in the sacral dorsal commissural nucleus of the rat. Neuroscience Letters, 388(3), 144-8.
Feng YP, et al. Morphological Evidence for GABA/glycine-cocontaining Terminals in Synaptic Contact With Neurokinin-1 Receptor-expressing Neurons in the Sacral Dorsal Commissural Nucleus of the Rat. Neurosci Lett. 2005 Nov 18;388(3):144-8. PubMed PMID: 16043285.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphological evidence for GABA/glycine-cocontaining terminals in synaptic contact with neurokinin-1 receptor-expressing neurons in the sacral dorsal commissural nucleus of the rat. AU - Feng,Yu-Peng, AU - Li,Yun-Qing, AU - Wang,Wen, AU - Wu,Sheng-Xi, AU - Chen,Tao, AU - Shigemoto,Ryuichi, AU - Mizuno,Noboru, PY - 2005/04/02/received PY - 2005/06/24/revised PY - 2005/06/25/accepted PY - 2005/7/27/pubmed PY - 2006/1/24/medline PY - 2005/7/27/entrez SP - 144 EP - 8 JF - Neuroscience letters JO - Neurosci. Lett. VL - 388 IS - 3 N2 - Previous studies have shown that neurons in the sacral dorsal commissural nucleus (SDCN) express neurokinin-1 receptor (NK1R) and can be modulated by the co-release of GABA and glycine (Gly) from single presynaptic terminal. These results raise the possibility that GABA/Gly-cocontaining terminals might make synaptic contacts with NK1R-expressing neurons in the SDCN. In order to provide morphological evidence for this hypothesis, the triple-immunohistochemical studies were performed in the SDCN. Triple-immunofluorescence histochemical study showed that some axon terminals in close association with NK1R-immunopositive (NK1R-ip) neurons in the SDCN were immunopositive for both glutamic acid decarboxylase (GAD) and glycine transporter 2 (GlyT2). In electron microscopic dual- and triple-immunohistochemistry for GAD/GlyT2, GAD/NK1R, GlyT2/NK1R, or GAD/GlyT2/NK1R also revealed dually labeled (GAD/GlyT2-ip) synaptic terminals upon SDCN neurons, as well as GAD- and/or GlyT2-ip axon terminals in synaptic contact with NK1R-ip SDCN neurons. These results suggested that some synaptic terminals upon NK1R-expressing SDCN neurons co-released both GABA and Gly. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/16043285/Morphological_evidence_for_GABA/glycine_cocontaining_terminals_in_synaptic_contact_with_neurokinin_1_receptor_expressing_neurons_in_the_sacral_dorsal_commissural_nucleus_of_the_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(05)00754-8 DB - PRIME DP - Unbound Medicine ER -