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Heat and mechanical hyperalgesia in mice model of cancer pain.
Pain. 2005 Sep; 117(1-2):19-29.PAIN

Abstract

We developed a mouse model of cancer pain to investigate its underlying mechanisms. SCC-7, squamous cell carcinoma (SCC) derived from C3H mice, was inoculated subcutaneously into either the plantar region or thigh in male C3H/Hej mice. Heat and mechanical sensitivity as well as spontaneous behavior were measured at the plantar surface of the ipsilateral hind paw after the inoculation. Inoculated sites were histologically examined, and the expression of capsaicin receptors (TRPV1) was examined in the dorsal root ganglia (DRG) to clarify their potential contribution to pain sensitivity. Inoculation of cancer cells induced marked heat hyperalgesia and mechanical allodynia in the ipsilateral hind paw for two weeks in both plantar- and thigh-inoculation models. Signs of spontaneous pain, such as lifting, licking and flinching of the paw were also observed. However, further growth of the tumor reversed the mechanical allodynia in both plantar- and thigh-inoculation models, and heat hyperalgesia in thigh-inoculation models. Histologically, no infiltration of the tumor cells into the nerve was observed. TRPV1 immunoreactive cells increased in the L5 DRG on day 7, but returned to the control level on day 15 post-inoculation. Intraperitoneal administration of the competitive TRPV1 antagonist capsazepine inhibited hyperalgesia induced by tumor cell-inoculation in either plantar- or thigh-inoculated animals. This study indicated that inoculation of SCC resulted in spontaneous pain, heat hyperalgesia and mechanical allodynia. The altered expression of TRPV1 in the DRG may be involved in behavioral changes in this model.

Authors+Show Affiliations

Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16043290

Citation

Asai, Hideaki, et al. "Heat and Mechanical Hyperalgesia in Mice Model of Cancer Pain." Pain, vol. 117, no. 1-2, 2005, pp. 19-29.
Asai H, Ozaki N, Shinoda M, et al. Heat and mechanical hyperalgesia in mice model of cancer pain. Pain. 2005;117(1-2):19-29.
Asai, H., Ozaki, N., Shinoda, M., Nagamine, K., Tohnai, I., Ueda, M., & Sugiura, Y. (2005). Heat and mechanical hyperalgesia in mice model of cancer pain. Pain, 117(1-2), 19-29.
Asai H, et al. Heat and Mechanical Hyperalgesia in Mice Model of Cancer Pain. Pain. 2005;117(1-2):19-29. PubMed PMID: 16043290.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heat and mechanical hyperalgesia in mice model of cancer pain. AU - Asai,Hideaki, AU - Ozaki,Noriyuki, AU - Shinoda,Masamichi, AU - Nagamine,Kenjiro, AU - Tohnai,Iwai, AU - Ueda,Minoru, AU - Sugiura,Yasuo, PY - 2004/11/24/received PY - 2005/03/14/revised PY - 2005/05/03/accepted PY - 2005/7/27/pubmed PY - 2006/1/6/medline PY - 2005/7/27/entrez SP - 19 EP - 29 JF - Pain JO - Pain VL - 117 IS - 1-2 N2 - We developed a mouse model of cancer pain to investigate its underlying mechanisms. SCC-7, squamous cell carcinoma (SCC) derived from C3H mice, was inoculated subcutaneously into either the plantar region or thigh in male C3H/Hej mice. Heat and mechanical sensitivity as well as spontaneous behavior were measured at the plantar surface of the ipsilateral hind paw after the inoculation. Inoculated sites were histologically examined, and the expression of capsaicin receptors (TRPV1) was examined in the dorsal root ganglia (DRG) to clarify their potential contribution to pain sensitivity. Inoculation of cancer cells induced marked heat hyperalgesia and mechanical allodynia in the ipsilateral hind paw for two weeks in both plantar- and thigh-inoculation models. Signs of spontaneous pain, such as lifting, licking and flinching of the paw were also observed. However, further growth of the tumor reversed the mechanical allodynia in both plantar- and thigh-inoculation models, and heat hyperalgesia in thigh-inoculation models. Histologically, no infiltration of the tumor cells into the nerve was observed. TRPV1 immunoreactive cells increased in the L5 DRG on day 7, but returned to the control level on day 15 post-inoculation. Intraperitoneal administration of the competitive TRPV1 antagonist capsazepine inhibited hyperalgesia induced by tumor cell-inoculation in either plantar- or thigh-inoculated animals. This study indicated that inoculation of SCC resulted in spontaneous pain, heat hyperalgesia and mechanical allodynia. The altered expression of TRPV1 in the DRG may be involved in behavioral changes in this model. SN - 0304-3959 UR - https://www.unboundmedicine.com/medline/citation/16043290/Heat_and_mechanical_hyperalgesia_in_mice_model_of_cancer_pain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3959(05)00218-6 DB - PRIME DP - Unbound Medicine ER -