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Soluble starch synthase I: a major determinant for the synthesis of amylopectin in Arabidopsis thaliana leaves.
Plant J. 2005 Aug; 43(3):398-412.PJ

Abstract

A minimum of four soluble starch synthase families have been documented in all starch-storing green plants. These activities are involved in amylopectin synthesis and are extremely well conserved throughout the plant kingdom. Mutants or transgenic plants defective for SSII and SSIII isoforms have been previously shown to have a large and specific impact on the synthesis of amylopectin while the function of the SSI type of enzymes has remained elusive. We report here that Arabidopsis mutants, lacking a plastidial starch synthase isoform belonging to the SSI family, display a major and novel type of structural alteration within their amylopectin. Comparative analysis of beta-limit dextrins for both wild type and mutant amylopectins suggests a specific and crucial function of SSI during the synthesis of transient starch in Arabidopsis leaves. Considering our own characterization of SSI activity and the previously described kinetic properties of maize SSI, our results suggest that the function of SSI is mainly involved in the synthesis of small outer chains during amylopectin cluster synthesis.

Authors+Show Affiliations

Delvallé D
Unité de Glycobiologie Structurale et Fonctionnelle, UMR8576 CNRS/USTL, IFR 118, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France.
Dumez S
No affiliation info available
Wattebled F
No affiliation info available
Roldán I
No affiliation info available
Planchot V
No affiliation info available
Berbezy P
No affiliation info available
Colonna P
No affiliation info available
Vyas D
No affiliation info available
Chatterjee M
No affiliation info available
Ball S
No affiliation info available
Mérida A
No affiliation info available
D'Hulst C
No affiliation info available

MeSH

AmylopectinArabidopsisChromatography, GelChromosome MappingEscherichia coliGene Expression Regulation, EnzymologicGene Expression Regulation, PlantKineticsMicroscopy, Electron, TransmissionPhenotypePlant LeavesRecombinant ProteinsStarch Synthase

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16045475

Citation

Delvallé, David, et al. "Soluble Starch Synthase I: a Major Determinant for the Synthesis of Amylopectin in Arabidopsis Thaliana Leaves." The Plant Journal : for Cell and Molecular Biology, vol. 43, no. 3, 2005, pp. 398-412.
Delvallé D, Dumez S, Wattebled F, et al. Soluble starch synthase I: a major determinant for the synthesis of amylopectin in Arabidopsis thaliana leaves. Plant J. 2005;43(3):398-412.
Delvallé, D., Dumez, S., Wattebled, F., Roldán, I., Planchot, V., Berbezy, P., Colonna, P., Vyas, D., Chatterjee, M., Ball, S., Mérida, A., & D'Hulst, C. (2005). Soluble starch synthase I: a major determinant for the synthesis of amylopectin in Arabidopsis thaliana leaves. The Plant Journal : for Cell and Molecular Biology, 43(3), 398-412.
Delvallé D, et al. Soluble Starch Synthase I: a Major Determinant for the Synthesis of Amylopectin in Arabidopsis Thaliana Leaves. Plant J. 2005;43(3):398-412. PubMed PMID: 16045475.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Soluble starch synthase I: a major determinant for the synthesis of amylopectin in Arabidopsis thaliana leaves. AU - Delvallé,David, AU - Dumez,Sylvain, AU - Wattebled,Fabrice, AU - Roldán,Isaac, AU - Planchot,Véronique, AU - Berbezy,Pierre, AU - Colonna,Paul, AU - Vyas,Darshna, AU - Chatterjee,Manash, AU - Ball,Steven, AU - Mérida,Angel, AU - D'Hulst,Christophe, PY - 2005/7/28/pubmed PY - 2005/12/13/medline PY - 2005/7/28/entrez SP - 398 EP - 412 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 43 IS - 3 N2 - A minimum of four soluble starch synthase families have been documented in all starch-storing green plants. These activities are involved in amylopectin synthesis and are extremely well conserved throughout the plant kingdom. Mutants or transgenic plants defective for SSII and SSIII isoforms have been previously shown to have a large and specific impact on the synthesis of amylopectin while the function of the SSI type of enzymes has remained elusive. We report here that Arabidopsis mutants, lacking a plastidial starch synthase isoform belonging to the SSI family, display a major and novel type of structural alteration within their amylopectin. Comparative analysis of beta-limit dextrins for both wild type and mutant amylopectins suggests a specific and crucial function of SSI during the synthesis of transient starch in Arabidopsis leaves. Considering our own characterization of SSI activity and the previously described kinetic properties of maize SSI, our results suggest that the function of SSI is mainly involved in the synthesis of small outer chains during amylopectin cluster synthesis. SN - 0960-7412 UR - https://www.unboundmedicine.com/medline/citation/16045475/Soluble_starch_synthase_I:_a_major_determinant_for_the_synthesis_of_amylopectin_in_Arabidopsis_thaliana_leaves_ DB - PRIME DP - Unbound Medicine ER -