Tags

Type your tag names separated by a space and hit enter

Positional isomers of sulfated oligosaccharides obtained from agarans and carrageenans: preparation and capillary electrophoresis separation.
Carbohydr Res. 2005 Sep 26; 340(13):2123-34.CR

Abstract

Partial reductive hydrolysis was used to produce oligosaccharide alditols from repetitive sulfated galactans obtained from four Rhodophyta species: kappa-carrageenan (from Kappaphycus alvarezii), theta-carrageenan (Gigartina skottsbergii-alkali-treated lambda-carrageenan), agarose 6-sulfate (Gracilaria domingensis), and pyruvylated agarose 2-sulfate (Acanthophora spicifera-alkali-treated pyruvylated agaran sulfate). Each hydrolyzate was submitted to anion-exchange and gel-filtration chromatography, and the isolated oligosaccharide alditols were identified by 1D and 2D NMR spectroscopy and by ESI mass spectrometry. The positional isomers of the sulfated oligosaccharide alditols were then completely resolved by capillary electrophoresis in a borate buffer. Attempts to correlate the availability of the hydroxyl groups for borate complexation with the relative migration of the oligosaccharides are presented.

Authors+Show Affiliations

Gonçalves AG
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Brazil.
Ducatti DR
No affiliation info available
Paranha RG
No affiliation info available
Eugênia M
No affiliation info available
Duarte R
No affiliation info available
Noseda MD
No affiliation info available

MeSH

AgarCarbohydrate SequenceCarrageenanElectrophoresis, CapillaryMolecular Sequence DataNuclear Magnetic Resonance, BiomolecularOligosaccharidesSepharoseSpectrometry, Mass, Electrospray IonizationSugar Alcohols

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16045901

Citation

Gonçalves, Alan G., et al. "Positional Isomers of Sulfated Oligosaccharides Obtained From Agarans and Carrageenans: Preparation and Capillary Electrophoresis Separation." Carbohydrate Research, vol. 340, no. 13, 2005, pp. 2123-34.
Gonçalves AG, Ducatti DR, Paranha RG, et al. Positional isomers of sulfated oligosaccharides obtained from agarans and carrageenans: preparation and capillary electrophoresis separation. Carbohydr Res. 2005;340(13):2123-34.
Gonçalves, A. G., Ducatti, D. R., Paranha, R. G., Eugênia, M., Duarte, R., & Noseda, M. D. (2005). Positional isomers of sulfated oligosaccharides obtained from agarans and carrageenans: preparation and capillary electrophoresis separation. Carbohydrate Research, 340(13), 2123-34.
Gonçalves AG, et al. Positional Isomers of Sulfated Oligosaccharides Obtained From Agarans and Carrageenans: Preparation and Capillary Electrophoresis Separation. Carbohydr Res. 2005 Sep 26;340(13):2123-34. PubMed PMID: 16045901.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Positional isomers of sulfated oligosaccharides obtained from agarans and carrageenans: preparation and capillary electrophoresis separation. AU - Gonçalves,Alan G, AU - Ducatti,Diogo R B, AU - Paranha,Reinaldo G, AU - Eugênia,M, AU - Duarte,R, AU - Noseda,Miguel D, PY - 2005/04/05/received PY - 2005/06/10/accepted PY - 2005/7/28/pubmed PY - 2005/12/13/medline PY - 2005/7/28/entrez SP - 2123 EP - 34 JF - Carbohydrate research JO - Carbohydr Res VL - 340 IS - 13 N2 - Partial reductive hydrolysis was used to produce oligosaccharide alditols from repetitive sulfated galactans obtained from four Rhodophyta species: kappa-carrageenan (from Kappaphycus alvarezii), theta-carrageenan (Gigartina skottsbergii-alkali-treated lambda-carrageenan), agarose 6-sulfate (Gracilaria domingensis), and pyruvylated agarose 2-sulfate (Acanthophora spicifera-alkali-treated pyruvylated agaran sulfate). Each hydrolyzate was submitted to anion-exchange and gel-filtration chromatography, and the isolated oligosaccharide alditols were identified by 1D and 2D NMR spectroscopy and by ESI mass spectrometry. The positional isomers of the sulfated oligosaccharide alditols were then completely resolved by capillary electrophoresis in a borate buffer. Attempts to correlate the availability of the hydroxyl groups for borate complexation with the relative migration of the oligosaccharides are presented. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/16045901/Positional_isomers_of_sulfated_oligosaccharides_obtained_from_agarans_and_carrageenans:_preparation_and_capillary_electrophoresis_separation_ DB - PRIME DP - Unbound Medicine ER -