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Efficiency of various serological techniques for diagnosing Coxiella burnetii infection.
Acta Virol. 2005; 49(2):123-7.AV

Abstract

An indirect immunofluorescence assay (IFA) using a recently developed commercial kit for detecting antibodies against Coxiella burnetii (C.b.), the etiological agent of Q fever, has been evaluated using human field serum samples. The IFA was compared with an ELISA and a complement fixation test (CFT). The IFA was based on the corpuscular C.b. phase I and phase II antigens specific to anti-C.b. phase I and II antibodies, respectively. Fifty sera from persons with symptoms of Q fever were examined in this study. The IFA compared with the ELISA showed the sensitivities of 97.7% and 87.2% for IgG and 66.7% and 60.0% for IgM phase II and I antibodies, respectively and the specificities of 100% and 90.0% for IgG and 75.9% and 64.7% for IgM phase II and phase I antibodies, respectively. Due to a limited number of sera positive in the IgA antibody testing, the data presented should be considered with caution. It appears that the IFA strikes a very good balance between high specificity and sensitivity with phase II and phase I IgG antibodies and a less satisfactory one with IgM antibodies. The CFT failed in one of the above aspects showing a good specificity but a poor sensitivity, especially for phase I antibodies. The study demonstrated that the IFA is suitable for diagnosing Q fever and its therapeutic follow-up and is a good candidate for screening sera in large numbers. A certain limitation, especially in testing early stages of the chronic disease, could be a low fluorescence intensity of the IgA positive control in comparison with the IgA negative one.

Authors+Show Affiliations

Laboratory for Diagnosis and Prevention of Rickettsial and Chlamydial Infections, Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 845 05 Bratislava, Slovak Republic.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16047740

Citation

Slabá, K, et al. "Efficiency of Various Serological Techniques for Diagnosing Coxiella Burnetii Infection." Acta Virologica, vol. 49, no. 2, 2005, pp. 123-7.
Slabá K, Skultéty L, Toman R. Efficiency of various serological techniques for diagnosing Coxiella burnetii infection. Acta Virol. 2005;49(2):123-7.
Slabá, K., Skultéty, L., & Toman, R. (2005). Efficiency of various serological techniques for diagnosing Coxiella burnetii infection. Acta Virologica, 49(2), 123-7.
Slabá K, Skultéty L, Toman R. Efficiency of Various Serological Techniques for Diagnosing Coxiella Burnetii Infection. Acta Virol. 2005;49(2):123-7. PubMed PMID: 16047740.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficiency of various serological techniques for diagnosing Coxiella burnetii infection. AU - Slabá,K, AU - Skultéty,L, AU - Toman,R, PY - 2005/7/29/pubmed PY - 2005/10/7/medline PY - 2005/7/29/entrez SP - 123 EP - 7 JF - Acta virologica JO - Acta Virol VL - 49 IS - 2 N2 - An indirect immunofluorescence assay (IFA) using a recently developed commercial kit for detecting antibodies against Coxiella burnetii (C.b.), the etiological agent of Q fever, has been evaluated using human field serum samples. The IFA was compared with an ELISA and a complement fixation test (CFT). The IFA was based on the corpuscular C.b. phase I and phase II antigens specific to anti-C.b. phase I and II antibodies, respectively. Fifty sera from persons with symptoms of Q fever were examined in this study. The IFA compared with the ELISA showed the sensitivities of 97.7% and 87.2% for IgG and 66.7% and 60.0% for IgM phase II and I antibodies, respectively and the specificities of 100% and 90.0% for IgG and 75.9% and 64.7% for IgM phase II and phase I antibodies, respectively. Due to a limited number of sera positive in the IgA antibody testing, the data presented should be considered with caution. It appears that the IFA strikes a very good balance between high specificity and sensitivity with phase II and phase I IgG antibodies and a less satisfactory one with IgM antibodies. The CFT failed in one of the above aspects showing a good specificity but a poor sensitivity, especially for phase I antibodies. The study demonstrated that the IFA is suitable for diagnosing Q fever and its therapeutic follow-up and is a good candidate for screening sera in large numbers. A certain limitation, especially in testing early stages of the chronic disease, could be a low fluorescence intensity of the IgA positive control in comparison with the IgA negative one. SN - 0001-723X UR - https://www.unboundmedicine.com/medline/citation/16047740/Efficiency_of_various_serological_techniques_for_diagnosing_Coxiella_burnetii_infection_ L2 - http://www.aepress.sk/_downloads/dl.php?from=pubmed&journal=AV&file=2005_02_123.pdf DB - PRIME DP - Unbound Medicine ER -