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Differential integration rates of hepatitis B virus DNA in the liver of children with chronic hepatitis B virus infection and hepatocellular carcinoma.
J Gastroenterol Hepatol. 2005 Aug; 20(8):1206-14.JG

Abstract

BACKGROUND AND AIM

Integration of hepatitis B virus-DNA (HBV-DNA) into the host genome, a phenomenon found frequently in hepatocellular carcinomas (HCC) and causally linked to oncogenesis, has not been well characterized in children. The aim of the present study was to determine the prevalence of HBV integration more accurately and to decide whether the integration rate varies at different stages of chronic HBV infection in children.

METHODS

Of 13 children with chronic hepatitis, 14 liver biopsy tissues were analyzed. One liver tissue with pure liver cirrhosis, nine non-tumor, and nine tumor liver tissues from children with HCC were analyzed by a very sensitive method, inverse polymerase chain reaction (IPCR).

RESULTS

Thirteen genuine viral-host junctional sequences from 23 patients were successfully isolated and proved that IPCR is a useful method in this context. The results also indicated that the detection rate of HBV-DNA integration increased in parallel with the progress of liver histology towards the neoplastic transformation, with 0% in the liver of chronic hepatitis, 22.2% in non-tumor livers of HCC patients, and 66.7% in tumor liver tissues of HCC patients.

CONCLUSION

The present results indicate that integration of HBV-DNA into the host genome was rarely confirmed at the early stage of chronic hepatitis in children until the stage of HCC formation.

Authors+Show Affiliations

Department of Pediatrics, College of Medicine, National Taiwan University, 10016 Taipei, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16048568

Citation

Huang, Hsiang-Po, et al. "Differential Integration Rates of Hepatitis B Virus DNA in the Liver of Children With Chronic Hepatitis B Virus Infection and Hepatocellular Carcinoma." Journal of Gastroenterology and Hepatology, vol. 20, no. 8, 2005, pp. 1206-14.
Huang HP, Tsuei DJ, Wang KJ, et al. Differential integration rates of hepatitis B virus DNA in the liver of children with chronic hepatitis B virus infection and hepatocellular carcinoma. J Gastroenterol Hepatol. 2005;20(8):1206-14.
Huang, H. P., Tsuei, D. J., Wang, K. J., Chen, Y. L., Ni, Y. H., Jeng, Y. M., Chen, H. L., Hsu, H. Y., & Chang, M. H. (2005). Differential integration rates of hepatitis B virus DNA in the liver of children with chronic hepatitis B virus infection and hepatocellular carcinoma. Journal of Gastroenterology and Hepatology, 20(8), 1206-14.
Huang HP, et al. Differential Integration Rates of Hepatitis B Virus DNA in the Liver of Children With Chronic Hepatitis B Virus Infection and Hepatocellular Carcinoma. J Gastroenterol Hepatol. 2005;20(8):1206-14. PubMed PMID: 16048568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential integration rates of hepatitis B virus DNA in the liver of children with chronic hepatitis B virus infection and hepatocellular carcinoma. AU - Huang,Hsiang-Po, AU - Tsuei,Daw-Jen, AU - Wang,Kuan-Jan, AU - Chen,Ying-Lien, AU - Ni,Yen-Hsuan, AU - Jeng,Yung-Ming, AU - Chen,Huey-Ling, AU - Hsu,Hong-Yuan, AU - Chang,Mei-Hwei, PY - 2005/7/29/pubmed PY - 2006/6/14/medline PY - 2005/7/29/entrez SP - 1206 EP - 14 JF - Journal of gastroenterology and hepatology JO - J Gastroenterol Hepatol VL - 20 IS - 8 N2 - BACKGROUND AND AIM: Integration of hepatitis B virus-DNA (HBV-DNA) into the host genome, a phenomenon found frequently in hepatocellular carcinomas (HCC) and causally linked to oncogenesis, has not been well characterized in children. The aim of the present study was to determine the prevalence of HBV integration more accurately and to decide whether the integration rate varies at different stages of chronic HBV infection in children. METHODS: Of 13 children with chronic hepatitis, 14 liver biopsy tissues were analyzed. One liver tissue with pure liver cirrhosis, nine non-tumor, and nine tumor liver tissues from children with HCC were analyzed by a very sensitive method, inverse polymerase chain reaction (IPCR). RESULTS: Thirteen genuine viral-host junctional sequences from 23 patients were successfully isolated and proved that IPCR is a useful method in this context. The results also indicated that the detection rate of HBV-DNA integration increased in parallel with the progress of liver histology towards the neoplastic transformation, with 0% in the liver of chronic hepatitis, 22.2% in non-tumor livers of HCC patients, and 66.7% in tumor liver tissues of HCC patients. CONCLUSION: The present results indicate that integration of HBV-DNA into the host genome was rarely confirmed at the early stage of chronic hepatitis in children until the stage of HCC formation. SN - 0815-9319 UR - https://www.unboundmedicine.com/medline/citation/16048568/Differential_integration_rates_of_hepatitis_B_virus_DNA_in_the_liver_of_children_with_chronic_hepatitis_B_virus_infection_and_hepatocellular_carcinoma_ L2 - https://doi.org/10.1111/j.1440-1746.2005.03789.x DB - PRIME DP - Unbound Medicine ER -