Tags

Type your tag names separated by a space and hit enter

Secretion of endolymph by the isolated frog semicircular canal.
Acta Otolaryngol. 1992; 112(2):294-8.AO

Abstract

Secretion of endolymph is localized in some structures of the inner ear, namely the stria vascularis in the cochlea and the dark cells in the vestibule and in the lower vertebrate inner ear. In isolated semicircular canal it is possible to study separately the endolymphatic composition in the ampulla, which contains the dark cells, and in its non-ampullar part, which is devoid of these cells. Further, in vitro preparation of the semicircular canal provides access to both faces of the epithelium so that different agents can be applied separately to the apical or to the basolateral membranes of the epithelium. In this structure, the following results were obtained: i) in vitro, the semicircular canal secreted a K-rich, positively polarized fluid; ii) this fluid was secreted only in the ampulla of the semicircular canal; iii) the secretion of endolymph was dependent on basolateral Na+, K(+)-ATPase, inhibited by ouabain, and basolateral Na-K-Cl co-transporter, inhibited by bumetanide; iv) approximately 60% of luminal Na absorption occurred across a luminal Na channel inhibited by amiloride; v) the permeability of the paracellular pathway of the semicircular canal epithelium was 7.10(-7) cm/s. These results indicate that endolymph secretion involves basolateral Na+, K(+)-ATPase and Na-K-Cl co-transporter. An Na channel has been shown at the apical membrane.

Authors+Show Affiliations

INSERM U.251, Faculté Xavier Bichat, Université Paris 7, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1604995

Citation

Ferrary, E, et al. "Secretion of Endolymph By the Isolated Frog Semicircular Canal." Acta Oto-laryngologica, vol. 112, no. 2, 1992, pp. 294-8.
Ferrary E, Bernard C, Oudar O, et al. Secretion of endolymph by the isolated frog semicircular canal. Acta Otolaryngol. 1992;112(2):294-8.
Ferrary, E., Bernard, C., Oudar, O., Sterkers, O., & Amiel, C. (1992). Secretion of endolymph by the isolated frog semicircular canal. Acta Oto-laryngologica, 112(2), 294-8.
Ferrary E, et al. Secretion of Endolymph By the Isolated Frog Semicircular Canal. Acta Otolaryngol. 1992;112(2):294-8. PubMed PMID: 1604995.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Secretion of endolymph by the isolated frog semicircular canal. AU - Ferrary,E, AU - Bernard,C, AU - Oudar,O, AU - Sterkers,O, AU - Amiel,C, PY - 1992/1/1/pubmed PY - 1992/1/1/medline PY - 1992/1/1/entrez SP - 294 EP - 8 JF - Acta oto-laryngologica JO - Acta Otolaryngol. VL - 112 IS - 2 N2 - Secretion of endolymph is localized in some structures of the inner ear, namely the stria vascularis in the cochlea and the dark cells in the vestibule and in the lower vertebrate inner ear. In isolated semicircular canal it is possible to study separately the endolymphatic composition in the ampulla, which contains the dark cells, and in its non-ampullar part, which is devoid of these cells. Further, in vitro preparation of the semicircular canal provides access to both faces of the epithelium so that different agents can be applied separately to the apical or to the basolateral membranes of the epithelium. In this structure, the following results were obtained: i) in vitro, the semicircular canal secreted a K-rich, positively polarized fluid; ii) this fluid was secreted only in the ampulla of the semicircular canal; iii) the secretion of endolymph was dependent on basolateral Na+, K(+)-ATPase, inhibited by ouabain, and basolateral Na-K-Cl co-transporter, inhibited by bumetanide; iv) approximately 60% of luminal Na absorption occurred across a luminal Na channel inhibited by amiloride; v) the permeability of the paracellular pathway of the semicircular canal epithelium was 7.10(-7) cm/s. These results indicate that endolymph secretion involves basolateral Na+, K(+)-ATPase and Na-K-Cl co-transporter. An Na channel has been shown at the apical membrane. SN - 0001-6489 UR - https://www.unboundmedicine.com/medline/citation/1604995/Secretion_of_endolymph_by_the_isolated_frog_semicircular_canal_ L2 - http://www.tandfonline.com/doi/full/10.1080/00016489.1992.11665421 DB - PRIME DP - Unbound Medicine ER -