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Macrophage migration inhibitory factor in the human epididymis and semen.
Mol Hum Reprod. 2005 Aug; 11(8):575-82.MH

Abstract

During epididymal transit, mammalian spermatozoa acquire new proteins involved in the acquisition of motility and of male gamete fertilising ability. We have previously shown that membranous vesicles called epididymosomes are involved in the transfer of epididymal-originating proteins to spermatozoa. The cytokine macrophage migration inhibitory factor (MIF) is one of these proteins but the role played by MIF in relation to epididymal sperm maturation still remains unclear. As this protein has already been shown to bear different functions depending on its location, we investigated its distribution along the epididymis and in different compartments of human semen. Northern and Western blot analysis as well as immunohistochemical studies show that MIF is expressed all along the epididymis with a higher level of transcript in the proximal segment. MIF is associated with two types of membranous vesicles, i.e. epididymosomes and prostasomes, the latter being prostate-originating membranous vesicles present in the semen. In semen, MIF is associated with spermatozoa, prostasomes as well as the soluble fraction. The amount of MIF in the seminal fluid varies from one individual to another but does not correlate with the amount of MIF associated with ejaculated spermatozoa. There is a negative correlation between the amount of sperm-associated MIF and the percentage of motility in different semen samples. Sperm separation using discontinuous Percoll gradient centrifugation shows a higher amount of MIF associated with poorly motile spermatozoa compared to highly motile spermatozoa present in the lower Percoll fraction. These results are discussed with regards to the possible involvement of MIF in sperm motility acquisition during the epididymal transit.

Authors+Show Affiliations

Centre de Recherche en Biologie de la Reproduction and Département d'Obstétrique-Gynécologie, Faculté de Médecine, Université Laval, Ste-Foy, Québec, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16051683

Citation

Frenette, Gilles, et al. "Macrophage Migration Inhibitory Factor in the Human Epididymis and Semen." Molecular Human Reproduction, vol. 11, no. 8, 2005, pp. 575-82.
Frenette G, Légaré C, Saez F, et al. Macrophage migration inhibitory factor in the human epididymis and semen. Mol Hum Reprod. 2005;11(8):575-82.
Frenette, G., Légaré, C., Saez, F., & Sullivan, R. (2005). Macrophage migration inhibitory factor in the human epididymis and semen. Molecular Human Reproduction, 11(8), 575-82.
Frenette G, et al. Macrophage Migration Inhibitory Factor in the Human Epididymis and Semen. Mol Hum Reprod. 2005;11(8):575-82. PubMed PMID: 16051683.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Macrophage migration inhibitory factor in the human epididymis and semen. AU - Frenette,Gilles, AU - Légaré,Christine, AU - Saez,Fabrice, AU - Sullivan,Robert, Y1 - 2005/07/28/ PY - 2005/7/30/pubmed PY - 2005/12/13/medline PY - 2005/7/30/entrez SP - 575 EP - 82 JF - Molecular human reproduction JO - Mol Hum Reprod VL - 11 IS - 8 N2 - During epididymal transit, mammalian spermatozoa acquire new proteins involved in the acquisition of motility and of male gamete fertilising ability. We have previously shown that membranous vesicles called epididymosomes are involved in the transfer of epididymal-originating proteins to spermatozoa. The cytokine macrophage migration inhibitory factor (MIF) is one of these proteins but the role played by MIF in relation to epididymal sperm maturation still remains unclear. As this protein has already been shown to bear different functions depending on its location, we investigated its distribution along the epididymis and in different compartments of human semen. Northern and Western blot analysis as well as immunohistochemical studies show that MIF is expressed all along the epididymis with a higher level of transcript in the proximal segment. MIF is associated with two types of membranous vesicles, i.e. epididymosomes and prostasomes, the latter being prostate-originating membranous vesicles present in the semen. In semen, MIF is associated with spermatozoa, prostasomes as well as the soluble fraction. The amount of MIF in the seminal fluid varies from one individual to another but does not correlate with the amount of MIF associated with ejaculated spermatozoa. There is a negative correlation between the amount of sperm-associated MIF and the percentage of motility in different semen samples. Sperm separation using discontinuous Percoll gradient centrifugation shows a higher amount of MIF associated with poorly motile spermatozoa compared to highly motile spermatozoa present in the lower Percoll fraction. These results are discussed with regards to the possible involvement of MIF in sperm motility acquisition during the epididymal transit. SN - 1360-9947 UR - https://www.unboundmedicine.com/medline/citation/16051683/Macrophage_migration_inhibitory_factor_in_the_human_epididymis_and_semen_ L2 - https://academic.oup.com/molehr/article-lookup/doi/10.1093/molehr/gah197 DB - PRIME DP - Unbound Medicine ER -