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Krox20 stimulates adipogenesis via C/EBPbeta-dependent and -independent mechanisms.
Cell Metab. 2005 Feb; 1(2):93-106.CM

Abstract

Krox20 is a zinc finger-containing transcription factor that is abundantly expressed in adipose tissue. However, its role in fat cell differentiation has not been established. In cultured 3T3-L1 cells, Krox20 is rapidly induced by serum stimulation. Overexpression of Krox20 in both 3T3-L1 preadipocytes and multipotent NIH3T3 cells promotes adipogenesis in a hormone-dependent manner. Conversely, RNAi-mediated loss of Krox20 function reduced adipogenesis in 3T3-L1 cells. Ectopic expression of Krox20 can transactivate the C/EBPbeta promoter and increase C/EBPbeta gene expression in 3T3-L1 preadipocytes. RNAi-mediated knockdown of C/EPBbeta diminished Krox20's proadipogenic effect. Finally, coexpression of Krox20 and C/EBPbeta in naive NIH3T3 cells resulted in the pronounced induction of a fully differentiated adipocyte phenotype, an effect previously observed only with PPARgamma. These data indicate that Krox20 is necessary for adipogenesis and that, when overexpressed, Krox20 potently stimulates adipogenesis via C/EBPbeta-dependent and -independent mechanisms.

Authors+Show Affiliations

Chen Z
Laboratory of Molecular Genetics, Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
Torrens JI
No affiliation info available
Anand A
No affiliation info available
Spiegelman BM
No affiliation info available
Friedman JM
No affiliation info available

MeSH

3T3-L1 CellsAdipocytesAnimalsBlotting, WesternCCAAT-Enhancer-Binding Protein-betaCell DifferentiationCell LineCell LineageDNA-Binding ProteinsEarly Growth Response Protein 2Gene SilencingGenes, ReporterHormonesLuciferasesMiceMolecular Sequence DataNIH 3T3 CellsPPAR gammaPhenotypePlasmidsPromoter Regions, GeneticRNA InterferenceRNA, Small InterferingRetroviridaeReverse Transcriptase Polymerase Chain ReactionTime FactorsTranscription FactorsTranscriptional ActivationTransfectionZinc Fingers

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16054051

Citation

Chen, Zhu, et al. "Krox20 Stimulates Adipogenesis Via C/EBPbeta-dependent and -independent Mechanisms." Cell Metabolism, vol. 1, no. 2, 2005, pp. 93-106.
Chen Z, Torrens JI, Anand A, et al. Krox20 stimulates adipogenesis via C/EBPbeta-dependent and -independent mechanisms. Cell Metab. 2005;1(2):93-106.
Chen, Z., Torrens, J. I., Anand, A., Spiegelman, B. M., & Friedman, J. M. (2005). Krox20 stimulates adipogenesis via C/EBPbeta-dependent and -independent mechanisms. Cell Metabolism, 1(2), 93-106.
Chen Z, et al. Krox20 Stimulates Adipogenesis Via C/EBPbeta-dependent and -independent Mechanisms. Cell Metab. 2005;1(2):93-106. PubMed PMID: 16054051.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Krox20 stimulates adipogenesis via C/EBPbeta-dependent and -independent mechanisms. AU - Chen,Zhu, AU - Torrens,Javier I, AU - Anand,Ashim, AU - Spiegelman,Bruce M, AU - Friedman,Jeffrey M, PY - 2004/03/16/received PY - 2004/11/22/revised PY - 2004/12/21/accepted PY - 2005/8/2/pubmed PY - 2005/9/15/medline PY - 2005/8/2/entrez SP - 93 EP - 106 JF - Cell metabolism JO - Cell Metab VL - 1 IS - 2 N2 - Krox20 is a zinc finger-containing transcription factor that is abundantly expressed in adipose tissue. However, its role in fat cell differentiation has not been established. In cultured 3T3-L1 cells, Krox20 is rapidly induced by serum stimulation. Overexpression of Krox20 in both 3T3-L1 preadipocytes and multipotent NIH3T3 cells promotes adipogenesis in a hormone-dependent manner. Conversely, RNAi-mediated loss of Krox20 function reduced adipogenesis in 3T3-L1 cells. Ectopic expression of Krox20 can transactivate the C/EBPbeta promoter and increase C/EBPbeta gene expression in 3T3-L1 preadipocytes. RNAi-mediated knockdown of C/EPBbeta diminished Krox20's proadipogenic effect. Finally, coexpression of Krox20 and C/EBPbeta in naive NIH3T3 cells resulted in the pronounced induction of a fully differentiated adipocyte phenotype, an effect previously observed only with PPARgamma. These data indicate that Krox20 is necessary for adipogenesis and that, when overexpressed, Krox20 potently stimulates adipogenesis via C/EBPbeta-dependent and -independent mechanisms. SN - 1550-4131 UR - https://www.unboundmedicine.com/medline/citation/16054051/Krox20_stimulates_adipogenesis_via_C/EBPbeta_dependent_and__independent_mechanisms_ DB - PRIME DP - Unbound Medicine ER -