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Lack of evidence that proline homozygosity at codon 72 of p53 and rare arginine allele at codon 31 of p21, jointly mediate cervical cancer susceptibility among Indian women.
Gynecol Oncol. 2005 Oct; 99(1):176-82.GO

Abstract

OBJECTIVES

The objective of this study was to identify whether variants of p53Arg72Pro and p21Ser31Arg were associated with increased risk for cervical cancer (CaCx), either independently or jointly, among Indian women.

METHODS

Genotyping was done by PCR-RFLP using DNA from (i) 120 cervical biopsy tissues of squamous cell carcinoma of the cervix (of which 82 were HPV16/18 positive), and (ii) a total of 205 cytologically normal cervical scrapes (121 HPV-negative and 84 HPV16/18-positive samples, considered as discreet groups). Multiple logistic regression analyses were performed to examine additive or interactive effects of the two factors and for determining age-adjusted OR (95% CI) and P values.

RESULTS

The observed association of proline homozygosity at codon 72 of p53 with CaCx infection (Bhattacharya, P., Duttagupta, C., Sengupta, S. 2002.Proline homozygosity in codon 72 of p53: A risk genotype for Human Papillomavirus related cervical cancer in Indian women. Cancer Lett 188: 207-211) was retained among Indian women harboring HPV16/18 (OR(age-adjusted) = 3.76; 95% CI = 1.03-13.80; P = 0.04). Significant independent association was evident between the p21 arginine allele (rare allele with frequency of 0.1) at codon 31 and CaCx, compared to HPV-negative cytologically normal controls (OR(age-adjusted) = 2.01; 95% CI = 1.00-4.06; P = 0.05). The two risk factors jointly failed to show statistical interaction towards susceptibility to CaCx. The p21 arginine allele was significantly associated with CaCx in the p53 proline non-homozygous group of subjects (OR(age-adjusted) = 2.68; 95% CI: 1.21-5.91; P = 0.01), and specifically in the p53 heterozygous group (OR(age-adjusted) = 2.91; 95% CI = 1.12-7.56; P = 0.03).

CONCLUSIONS

p53 and p21 act in series in mediating cell cycle arrest. However, the two risk factors, p53 proline homozygosity and p21 arginine allele, although part of a common causal pathway, appear to act in a mutually exclusive manner.

Authors+Show Affiliations

Human Genetics Unit, Indian Statistical Institute, 203 B.T. Road, Kolkata 700 108, India.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16054204

Citation

Bhattacharya, Paramita, and Sharmila Sengupta. "Lack of Evidence That Proline Homozygosity at Codon 72 of P53 and Rare Arginine Allele at Codon 31 of P21, Jointly Mediate Cervical Cancer Susceptibility Among Indian Women." Gynecologic Oncology, vol. 99, no. 1, 2005, pp. 176-82.
Bhattacharya P, Sengupta S. Lack of evidence that proline homozygosity at codon 72 of p53 and rare arginine allele at codon 31 of p21, jointly mediate cervical cancer susceptibility among Indian women. Gynecol Oncol. 2005;99(1):176-82.
Bhattacharya, P., & Sengupta, S. (2005). Lack of evidence that proline homozygosity at codon 72 of p53 and rare arginine allele at codon 31 of p21, jointly mediate cervical cancer susceptibility among Indian women. Gynecologic Oncology, 99(1), 176-82.
Bhattacharya P, Sengupta S. Lack of Evidence That Proline Homozygosity at Codon 72 of P53 and Rare Arginine Allele at Codon 31 of P21, Jointly Mediate Cervical Cancer Susceptibility Among Indian Women. Gynecol Oncol. 2005;99(1):176-82. PubMed PMID: 16054204.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lack of evidence that proline homozygosity at codon 72 of p53 and rare arginine allele at codon 31 of p21, jointly mediate cervical cancer susceptibility among Indian women. AU - Bhattacharya,Paramita, AU - Sengupta,Sharmila, PY - 2004/12/30/received PY - 2005/05/17/revised PY - 2005/06/06/accepted PY - 2005/8/2/pubmed PY - 2005/11/5/medline PY - 2005/8/2/entrez SP - 176 EP - 82 JF - Gynecologic oncology JO - Gynecol Oncol VL - 99 IS - 1 N2 - OBJECTIVES: The objective of this study was to identify whether variants of p53Arg72Pro and p21Ser31Arg were associated with increased risk for cervical cancer (CaCx), either independently or jointly, among Indian women. METHODS: Genotyping was done by PCR-RFLP using DNA from (i) 120 cervical biopsy tissues of squamous cell carcinoma of the cervix (of which 82 were HPV16/18 positive), and (ii) a total of 205 cytologically normal cervical scrapes (121 HPV-negative and 84 HPV16/18-positive samples, considered as discreet groups). Multiple logistic regression analyses were performed to examine additive or interactive effects of the two factors and for determining age-adjusted OR (95% CI) and P values. RESULTS: The observed association of proline homozygosity at codon 72 of p53 with CaCx infection (Bhattacharya, P., Duttagupta, C., Sengupta, S. 2002.Proline homozygosity in codon 72 of p53: A risk genotype for Human Papillomavirus related cervical cancer in Indian women. Cancer Lett 188: 207-211) was retained among Indian women harboring HPV16/18 (OR(age-adjusted) = 3.76; 95% CI = 1.03-13.80; P = 0.04). Significant independent association was evident between the p21 arginine allele (rare allele with frequency of 0.1) at codon 31 and CaCx, compared to HPV-negative cytologically normal controls (OR(age-adjusted) = 2.01; 95% CI = 1.00-4.06; P = 0.05). The two risk factors jointly failed to show statistical interaction towards susceptibility to CaCx. The p21 arginine allele was significantly associated with CaCx in the p53 proline non-homozygous group of subjects (OR(age-adjusted) = 2.68; 95% CI: 1.21-5.91; P = 0.01), and specifically in the p53 heterozygous group (OR(age-adjusted) = 2.91; 95% CI = 1.12-7.56; P = 0.03). CONCLUSIONS: p53 and p21 act in series in mediating cell cycle arrest. However, the two risk factors, p53 proline homozygosity and p21 arginine allele, although part of a common causal pathway, appear to act in a mutually exclusive manner. SN - 0090-8258 UR - https://www.unboundmedicine.com/medline/citation/16054204/Lack_of_evidence_that_proline_homozygosity_at_codon_72_of_p53_and_rare_arginine_allele_at_codon_31_of_p21_jointly_mediate_cervical_cancer_susceptibility_among_Indian_women_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0090-8258(05)00424-5 DB - PRIME DP - Unbound Medicine ER -