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Serotypes, virulence genes and intimin types of verotoxin-producing Escherichia coli and enteropathogenic E. coli isolated from healthy dairy goats in Spain.
Vet Microbiol. 2005 Sep 30; 110(1-2):67-76.VM

Abstract

Faecal samples from 222 healthy dairy goats on 12 farms in Spain, as well as bulk tank milk samples of these farms, were screened for the presence of verotoxin-producing Escherichia coli (VTEC) and enteropathogenic E. coli (EPEC). VTEC and EPEC were isolated in 47.7 and 7.7% of the animals, respectively. VTEC were isolated more frequently from adults and replacement animals than from goat kids. In contrast, EPEC were detected more frequently from goat kids than from replacement animals and adults. VTEC or EPEC strains were not detected in the bulk tank milk samples. Although a selective enrichment protocol was used, the serotype O157:H7 was not detected. The most frequent serotypes among the 106 VTEC strains isolated from goats were O5:H-, O76:H19, O126:H8, O146:H21, ONT:H- and ONT:H21. None VTEC strain was eae-positive. The absence of the eae gene in the VTEC strains could indicate that these strains are less virulent for humans that the classical eae-positive enterohaemorrhagic E. coli types. However, 16% of VTEC strains isolated from healthy goats belonged to serotypes associated with haemolytic uraemic syndrome in humans. The ehxA gene was detected in 84.9 and 52.9% of the VTEC and EPEC from goats, respectively. The beta1, theta/gamma2 and zeta were the most frequent intimin types among the 17 EPEC strains studied and the most prevalent serotypes of these strains were O156:H25 and O177:H11. Our data show that in Spain healthy goats are an important reservoir of VTEC and EPEC, and a potential source of infection for humans.

Authors+Show Affiliations

Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16054307

Citation

Cortés, C, et al. "Serotypes, Virulence Genes and Intimin Types of Verotoxin-producing Escherichia Coli and Enteropathogenic E. Coli Isolated From Healthy Dairy Goats in Spain." Veterinary Microbiology, vol. 110, no. 1-2, 2005, pp. 67-76.
Cortés C, De la Fuente R, Blanco J, et al. Serotypes, virulence genes and intimin types of verotoxin-producing Escherichia coli and enteropathogenic E. coli isolated from healthy dairy goats in Spain. Vet Microbiol. 2005;110(1-2):67-76.
Cortés, C., De la Fuente, R., Blanco, J., Blanco, M., Blanco, J. E., Dhabi, G., Mora, A., Justel, P., Contreras, A., Sánchez, A., Corrales, J. C., & Orden, J. A. (2005). Serotypes, virulence genes and intimin types of verotoxin-producing Escherichia coli and enteropathogenic E. coli isolated from healthy dairy goats in Spain. Veterinary Microbiology, 110(1-2), 67-76.
Cortés C, et al. Serotypes, Virulence Genes and Intimin Types of Verotoxin-producing Escherichia Coli and Enteropathogenic E. Coli Isolated From Healthy Dairy Goats in Spain. Vet Microbiol. 2005 Sep 30;110(1-2):67-76. PubMed PMID: 16054307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serotypes, virulence genes and intimin types of verotoxin-producing Escherichia coli and enteropathogenic E. coli isolated from healthy dairy goats in Spain. AU - Cortés,C, AU - De la Fuente,R, AU - Blanco,J, AU - Blanco,M, AU - Blanco,J E, AU - Dhabi,G, AU - Mora,A, AU - Justel,P, AU - Contreras,A, AU - Sánchez,A, AU - Corrales,J C, AU - Orden,J A, PY - 2005/05/09/received PY - 2005/06/10/revised PY - 2005/06/24/accepted PY - 2005/8/2/pubmed PY - 2006/1/6/medline PY - 2005/8/2/entrez SP - 67 EP - 76 JF - Veterinary microbiology JO - Vet Microbiol VL - 110 IS - 1-2 N2 - Faecal samples from 222 healthy dairy goats on 12 farms in Spain, as well as bulk tank milk samples of these farms, were screened for the presence of verotoxin-producing Escherichia coli (VTEC) and enteropathogenic E. coli (EPEC). VTEC and EPEC were isolated in 47.7 and 7.7% of the animals, respectively. VTEC were isolated more frequently from adults and replacement animals than from goat kids. In contrast, EPEC were detected more frequently from goat kids than from replacement animals and adults. VTEC or EPEC strains were not detected in the bulk tank milk samples. Although a selective enrichment protocol was used, the serotype O157:H7 was not detected. The most frequent serotypes among the 106 VTEC strains isolated from goats were O5:H-, O76:H19, O126:H8, O146:H21, ONT:H- and ONT:H21. None VTEC strain was eae-positive. The absence of the eae gene in the VTEC strains could indicate that these strains are less virulent for humans that the classical eae-positive enterohaemorrhagic E. coli types. However, 16% of VTEC strains isolated from healthy goats belonged to serotypes associated with haemolytic uraemic syndrome in humans. The ehxA gene was detected in 84.9 and 52.9% of the VTEC and EPEC from goats, respectively. The beta1, theta/gamma2 and zeta were the most frequent intimin types among the 17 EPEC strains studied and the most prevalent serotypes of these strains were O156:H25 and O177:H11. Our data show that in Spain healthy goats are an important reservoir of VTEC and EPEC, and a potential source of infection for humans. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/16054307/Serotypes_virulence_genes_and_intimin_types_of_verotoxin_producing_Escherichia_coli_and_enteropathogenic_E__coli_isolated_from_healthy_dairy_goats_in_Spain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(05)00211-7 DB - PRIME DP - Unbound Medicine ER -