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A-type cranberry proanthocyanidins and uropathogenic bacterial anti-adhesion activity.
Phytochemistry 2005; 66(18):2281-91P

Abstract

Clinical, epidemiological and mechanistic studies support the role of cranberry (Vaccinium macrocarpon Ait.) in maintaining urinary tract health. Cranberry proanthocyanidins contain A-type linkages and have been associated with preventing adhesion of P-fimbriated uropathogenic Escherichia coli to uroepithelial cells. It is not known if the presence of the A-type linkage is a prerequisite for anti-adhesion activity. Other commercial sources of proanthocyanidins with all B-type linkages have not previously been screened for this activity. The goals of this study were to compare the in vitro anti-adhesion activity of A-linked proanthocyanidins from cranberry juice cocktail with the anti-adhesion activities of B-linked proanthocyanidins from commercial grape and apple juices, green tea and dark chocolate, and determine if anti-adhesion activity is detectable in human urine following consumption of single servings of each commercial food product. Structural heterogeneity and presence of the A-type linkage in cranberry proanthocyanidins was confirmed utilizing MALDI-TOF/MS and DI/ESI MS, as was the presence of all B-type linkages in the proanthocyanidins from the other commercial products. The isolated A-type proanthocyanidins from cranberry juice cocktail elicited in vitro anti-adhesion activity at 60 microg/ml, the B-type proanthocyanidins from grape exhibited minor activity at 1200 microg/ml, while other B-type proanthocyanidins were not active. Anti-adhesion activity in human urine was detected following cranberry juice cocktail consumption, but not after consumption of the non-cranberry food products. Results suggest that presence of the A-type linkage in cranberry proanthocyanidins may enhance both in vitro and urinary bacterial anti-adhesion activities and aid in maintaining urinary tract health.

Authors+Show Affiliations

Marucci Center for Blueberry Cranberry Research, Rutgers University, Chatsworth, NJ 08019, USA. ahowell@aesop.rutgers.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Controlled Clinical Trial
Journal Article

Language

eng

PubMed ID

16055161

Citation

Howell, Amy B., et al. "A-type Cranberry Proanthocyanidins and Uropathogenic Bacterial Anti-adhesion Activity." Phytochemistry, vol. 66, no. 18, 2005, pp. 2281-91.
Howell AB, Reed JD, Krueger CG, et al. A-type cranberry proanthocyanidins and uropathogenic bacterial anti-adhesion activity. Phytochemistry. 2005;66(18):2281-91.
Howell, A. B., Reed, J. D., Krueger, C. G., Winterbottom, R., Cunningham, D. G., & Leahy, M. (2005). A-type cranberry proanthocyanidins and uropathogenic bacterial anti-adhesion activity. Phytochemistry, 66(18), pp. 2281-91.
Howell AB, et al. A-type Cranberry Proanthocyanidins and Uropathogenic Bacterial Anti-adhesion Activity. Phytochemistry. 2005;66(18):2281-91. PubMed PMID: 16055161.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A-type cranberry proanthocyanidins and uropathogenic bacterial anti-adhesion activity. AU - Howell,Amy B, AU - Reed,Jess D, AU - Krueger,Christian G, AU - Winterbottom,Ranee, AU - Cunningham,David G, AU - Leahy,Marge, PY - 2004/08/30/received PY - 2005/04/06/revised PY - 2005/8/2/pubmed PY - 2006/3/9/medline PY - 2005/8/2/entrez SP - 2281 EP - 91 JF - Phytochemistry JO - Phytochemistry VL - 66 IS - 18 N2 - Clinical, epidemiological and mechanistic studies support the role of cranberry (Vaccinium macrocarpon Ait.) in maintaining urinary tract health. Cranberry proanthocyanidins contain A-type linkages and have been associated with preventing adhesion of P-fimbriated uropathogenic Escherichia coli to uroepithelial cells. It is not known if the presence of the A-type linkage is a prerequisite for anti-adhesion activity. Other commercial sources of proanthocyanidins with all B-type linkages have not previously been screened for this activity. The goals of this study were to compare the in vitro anti-adhesion activity of A-linked proanthocyanidins from cranberry juice cocktail with the anti-adhesion activities of B-linked proanthocyanidins from commercial grape and apple juices, green tea and dark chocolate, and determine if anti-adhesion activity is detectable in human urine following consumption of single servings of each commercial food product. Structural heterogeneity and presence of the A-type linkage in cranberry proanthocyanidins was confirmed utilizing MALDI-TOF/MS and DI/ESI MS, as was the presence of all B-type linkages in the proanthocyanidins from the other commercial products. The isolated A-type proanthocyanidins from cranberry juice cocktail elicited in vitro anti-adhesion activity at 60 microg/ml, the B-type proanthocyanidins from grape exhibited minor activity at 1200 microg/ml, while other B-type proanthocyanidins were not active. Anti-adhesion activity in human urine was detected following cranberry juice cocktail consumption, but not after consumption of the non-cranberry food products. Results suggest that presence of the A-type linkage in cranberry proanthocyanidins may enhance both in vitro and urinary bacterial anti-adhesion activities and aid in maintaining urinary tract health. SN - 0031-9422 UR - https://www.unboundmedicine.com/medline/citation/16055161/A_type_cranberry_proanthocyanidins_and_uropathogenic_bacterial_anti_adhesion_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0031-9422(05)00249-9 DB - PRIME DP - Unbound Medicine ER -