[Quantitative analysis of pulegone in Herba Schizonepetae].Zhongguo Zhong Yao Za Zhi. 2005 May; 30(9):669-70.ZZ
To establish the determination method of pulegone in Herba Schizonepetae.
HPLC method was developed for the determination of pulegone in Herba Schizonepetae. The separation was performed on C18 column with MeOH-H2O (80:20) as a mobile phase. The detection wavelength was set at 252 nm.
The quantitation of pulegone in Herba Schizonepetae varied from 0.2-0.7 mg x g(-1). The average recovery is 96.2%, RSD 0.81%.
The method is simple, rapid and accurate.