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Validation of basophil histamine release against the autologous serum skin test and outcome of serum-induced basophil histamine release studies in a large population of chronic urticaria patients.
Allergy. 2005 Sep; 60(9):1152-6.A

Abstract

BACKGROUND

Endogenous histamine-releasing factors (HRFs) are involved in 30-60% of patients with chronic urticaria (CU). Evidence for their existence comes from in vivo studies of autoreactivity with the autologous serum skin test (ASST), in vitro immunoassays demonstrating autoantibodies against the immunoglobulin E (IgE) or the high affinity IgE receptor (FcepsilonRI) and serum-induced histamine release (HR) from basophils and mast cells. We have examined the correlation between the ASST and a new basophil histamine-releasing assay (the HR-Urtikaria test) in a group of well-characterized CU patients and subsequently determined the frequency of HR-Urticaria-positive sera from a larger population of CU patients.

SUBJECTS

Group 1 consisted of 28 patients with CU (16 were ASST-positive) 20 patients with atopic dermatitis, 24 patients with allergy to birch and nine healthy controls. Group 2 consisted of 873 unselected CU patients.

METHODS

White blood cells containing 1-2% basophils from a healthy nonatopic donor were incubated with patients sera in the presence of interleukin (IL)-3. Histamine was measured by the glass fibre method.

RESULTS

Using the ASST as the true outcome, the HR-Urticaria test showed a sensitivity and specificity of 75% in group 1 using a cut-off value for HR of >16.5%. None of the controls was positive in the HR-Urticaria test. In group 2, we found no difference in the frequency of positives between male (34.6%, n = 254) and female adults (35.1%, n = 576) but twice as many females as males were tested.

CONCLUSIONS

Our studies have shown that the HR-Urticaria test has a good sensitivity and specificity for endogenous HRFs demonstrated by the ASST in patients with CU and that about one-third of unselected patients with CU have a positive result.

Authors+Show Affiliations

National University Hospital, Allergy Clinic, Laboratory of Medical Allergology, Copenhagen, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

16076300

Citation

Platzer, M H., et al. "Validation of Basophil Histamine Release Against the Autologous Serum Skin Test and Outcome of Serum-induced Basophil Histamine Release Studies in a Large Population of Chronic Urticaria Patients." Allergy, vol. 60, no. 9, 2005, pp. 1152-6.
Platzer MH, Grattan CE, Poulsen LK, et al. Validation of basophil histamine release against the autologous serum skin test and outcome of serum-induced basophil histamine release studies in a large population of chronic urticaria patients. Allergy. 2005;60(9):1152-6.
Platzer, M. H., Grattan, C. E., Poulsen, L. K., & Skov, P. S. (2005). Validation of basophil histamine release against the autologous serum skin test and outcome of serum-induced basophil histamine release studies in a large population of chronic urticaria patients. Allergy, 60(9), 1152-6.
Platzer MH, et al. Validation of Basophil Histamine Release Against the Autologous Serum Skin Test and Outcome of Serum-induced Basophil Histamine Release Studies in a Large Population of Chronic Urticaria Patients. Allergy. 2005;60(9):1152-6. PubMed PMID: 16076300.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validation of basophil histamine release against the autologous serum skin test and outcome of serum-induced basophil histamine release studies in a large population of chronic urticaria patients. AU - Platzer,M H, AU - Grattan,C E H, AU - Poulsen,L K, AU - Skov,P S, PY - 2005/8/4/pubmed PY - 2005/11/4/medline PY - 2005/8/4/entrez SP - 1152 EP - 6 JF - Allergy JO - Allergy VL - 60 IS - 9 N2 - BACKGROUND: Endogenous histamine-releasing factors (HRFs) are involved in 30-60% of patients with chronic urticaria (CU). Evidence for their existence comes from in vivo studies of autoreactivity with the autologous serum skin test (ASST), in vitro immunoassays demonstrating autoantibodies against the immunoglobulin E (IgE) or the high affinity IgE receptor (FcepsilonRI) and serum-induced histamine release (HR) from basophils and mast cells. We have examined the correlation between the ASST and a new basophil histamine-releasing assay (the HR-Urtikaria test) in a group of well-characterized CU patients and subsequently determined the frequency of HR-Urticaria-positive sera from a larger population of CU patients. SUBJECTS: Group 1 consisted of 28 patients with CU (16 were ASST-positive) 20 patients with atopic dermatitis, 24 patients with allergy to birch and nine healthy controls. Group 2 consisted of 873 unselected CU patients. METHODS: White blood cells containing 1-2% basophils from a healthy nonatopic donor were incubated with patients sera in the presence of interleukin (IL)-3. Histamine was measured by the glass fibre method. RESULTS: Using the ASST as the true outcome, the HR-Urticaria test showed a sensitivity and specificity of 75% in group 1 using a cut-off value for HR of >16.5%. None of the controls was positive in the HR-Urticaria test. In group 2, we found no difference in the frequency of positives between male (34.6%, n = 254) and female adults (35.1%, n = 576) but twice as many females as males were tested. CONCLUSIONS: Our studies have shown that the HR-Urticaria test has a good sensitivity and specificity for endogenous HRFs demonstrated by the ASST in patients with CU and that about one-third of unselected patients with CU have a positive result. SN - 0105-4538 UR - https://www.unboundmedicine.com/medline/citation/16076300/Validation_of_basophil_histamine_release_against_the_autologous_serum_skin_test_and_outcome_of_serum_induced_basophil_histamine_release_studies_in_a_large_population_of_chronic_urticaria_patients_ DB - PRIME DP - Unbound Medicine ER -